Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme (original) (raw)
Lin, X. H. et al. Protein phosphatase 2A is required for the initiation of chromosomal DNA replication. Proc. Natl Acad. Sci. USA95, 14693–14698 (1998) ArticleADSCAS Google Scholar
Depaoli-Roach, A. A. et al. Serine/threonine protein phosphatases in the control of cell function. Adv. Enzyme Regul.34, 199–224 (1994) ArticleCAS Google Scholar
Sontag, E. Protein phosphatase 2A: the Trojan Horse of cellular signaling. Cell. Signal.13, 7–16 (2001) ArticleCAS Google Scholar
Janssens, V. & Goris, J. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J.353, 417–439 (2001) ArticleCAS Google Scholar
Goldberg, Y. Protein phosphatase 2A: who shall regulate the regulator?. Biochem. Pharmacol.57, 321–328 (1999) ArticleCAS Google Scholar
Virshup, D. M. Protein phosphatase 2A: a panoply of enzymes. Curr. Opin. Cell Biol.12, 180–185 (2000) ArticleCAS Google Scholar
Ogris, E., Gibson, D. M. & Pallas, D. C. Protein phosphatase 2A subunit assembly: the catalytic subunit carboxy terminus is important for binding cellular B subunit but not polyomavirus middle tumor antigen. Oncogene15, 911–917 (1997) ArticleCAS Google Scholar
Tolstykh, T., Lee, J., Vafai, S. & Stock, J. B. Carboxyl methylation regulates phosphoprotein phosphatase 2A by controlling the association of regulatory B subunits. EMBO J.19, 5682–5691 (2000) ArticleCAS Google Scholar
Wu, J. et al. Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo.. EMBO J.19, 5672–5681 (2000) ArticleCAS Google Scholar
Yu, X. X. et al. Methylation of the protein phosphatase 2A catalytic subunit is essential for association of Bα regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen. Mol. Biol. Cell12, 185–199 (2001) ArticleCAS Google Scholar
Wei, H. et al. Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J. Biol. Chem.276, 1570–1577 (2001) ArticleCAS Google Scholar
Turowski, P., Fernandez, A., Favre, B., Lamb, N. J. & Hemmings, B. A. Differential methylation and altered conformation of cytoplasmic and nuclear forms of protein phosphatase 2A during cell cycle progression. J. Cell Biol.129, 397–410 (1995) ArticleCAS Google Scholar
Sontag, E. et al. Downregulation of protein phosphatase 2A carboxyl methylation and methyltransferase may contribute to Alzheimer disease pathogenesis. J. Neuropathol. Exp. Neurol.63, 1080–1091 (2004) ArticleCAS Google Scholar
Janssens, V., Goris, J. & Van Hoof, C. PP2A: the expected tumor suppressor. Curr. Opin. Genet. Dev.15, 34–41 (2005) ArticleCAS Google Scholar
Letourneux, C., Rocher, G. & Porteu, F. B56-containing PP2A dephosphorylate ERK and their activity is controlled by the early gene IEX-1 and ERK. EMBO J.25, 727–738 (2006) ArticleCAS Google Scholar
Ito, A. et al. A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation. EMBO J.19, 562–571 (2000) ArticleCAS Google Scholar
Seeling, J. M. et al. Regulation of β-catenin signaling by the B56 subunit of protein phosphatase 2A. Science283, 2089–2091 (1999) ArticleADSCAS Google Scholar
McCright, B. & Virshup, D. M. Identification of a new family of protein phosphatase 2A regulatory subunits. J. Biol. Chem.270, 26123–26128 (1995) ArticleCAS Google Scholar
Chen, W. et al. Identification of specific PP2A complexes involved in human cell transformation. Cancer Cell5, 127–136 (2004) ArticleCAS Google Scholar
Perrotti, D. & Neviani, P. ReSETting PP2A tumour suppressor activity in blast crisis and imatinib-resistant chronic myelogenous leukaemia. Br. J. Cancer95, 775–781 (2006) ArticleCAS Google Scholar
Goldberg, J. et al. Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1. Nature376, 745–753 (1995) ArticleADSCAS Google Scholar
Groves, M. R., Hanlon, N., Turowski, P., Hemmings, B. A. & Barford, D. The structure of the protein phosphatase 2A PR65/A subunit reveals the conformation of its 15 tandemly repeated HEAT motifs. Cell96, 99–110 (1999) ArticleCAS Google Scholar
Griffith, J. P. et al. X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12–FK506 complex. Cell82, 507–522 (1995) ArticleCAS Google Scholar
Cai, L., Chu, Y., Wilson, S. E. & Schlender, K. K. A metal-dependent form of protein phosphatase 2A. Biochem. Biophys. Res. Commun.208, 274–279 (1995) ArticleCAS Google Scholar
Kremmer, E., Ohst, K., Kiefer, J., Brewis, N. & Walter, G. Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells. Mol. Cell. Biol.17, 1692–1701 (1997) ArticleCAS Google Scholar
Ruediger, R. et al. Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus. Mol. Cell. Biol.12, 4872–4882 (1992) ArticleCAS Google Scholar
Ruediger, R., Pham, H. T. & Walter, G. Disruption of protein phosphatase 2A subunit interaction in human cancers with mutations in the Aα subunit gene. Oncogene20, 10–15 (2001) ArticleCAS Google Scholar
Turowski, P., Favre, B., Campbell, K. S., Lamb, N. J. & Hemmings, B. A. Modulation of the enzymatic properties of protein phosphatase 2A catalytic subunit by the recombinant 65-kDa regulatory subunit PR65α. Eur. J. Biochem.248, 200–208 (1997) ArticleCAS Google Scholar
Wang, S. S. et al. Alterations of the PPP2R1B gene in human lung and colon cancer. Science282, 284–287 (1998) ArticleADSCAS Google Scholar
Ruediger, R., Fields, K. & Walter, G. Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits and T antigens. J. Virol.73, 839–842 (1999) CASPubMedPubMed Central Google Scholar
Ikehara, T., Shinjo, F., Ikehara, S., Imamura, S. & Yasumoto, T. Baculovirus expression, purification, and characterization of human protein phosphatase 2A catalytic subunits α and β. Protein Expr. Purif.45, 150–156 (2006) ArticleCAS Google Scholar
Philips, M. R. Methotrexate and Ras methylation: a new trick for an old drug?. Sci. STKE2004, pe13 (2004) PubMed Google Scholar
Chen, J., Martin, B. L. & Brautigan, D. L. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science257, 1261–1264 (1992) ArticleADSCAS Google Scholar
Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol.276, 307–326 (1997) ArticleCAS Google Scholar
Smith, G. D. et al. The use of SnB to determine an anomalous scattering substructure. Acta Crystallogr. D54, 799–804 (1998) ArticleCAS Google Scholar
De La Fortelle, E. & Bricogne, G. Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods. Methods Enzymol.276, 472–494 (1997) ArticleCAS Google Scholar
Cowtan, K. DM: an automated procedure for phase improvement by density modification. Joint CCP4 ESF-EACBM Newsl. Prot. Crystallogr.31, 34–38 (1994) Google Scholar
McRee, D. E. XtalView/Xfit–A versatile program for manipulating atomic coordinates and electron density. J. Struct. Biol.125, 156–165 (1999) ArticleCAS Google Scholar
Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D60, 2126–2132 (2004) Article Google Scholar
Murshudov, G. N., Vagin, A. A., Lebedev, A., Wilson, K. S. & Dodson, E. J. Efficient anisotropic refinement of macromolecular structures using FFT. Acta Crystallogr. D55, 247–255 (1999) ArticleCAS Google Scholar