The active form of DNA polymerase V is UmuD′2C–RecA–ATP (original) (raw)

References

  1. Tang, M. J. et al. UmuD′2C is an error-prone DNA polymerase, Escherichia coli pol V. Proc. Natl Acad. Sci. USA 96, 8919–8924 (1999)
    Article ADS CAS Google Scholar
  2. Reuven, N. B., Arad, G., Maor-Shoshani, A. & Livneh, Z. The mutagenesis protein UmuC is a DNA polymerase activated by UmuD′, RecA, and SSB and is specialized for translesion replication. J. Biol. Chem. 274, 31763–31766 (1999)
    Article CAS Google Scholar
  3. Friedberg, E. C. et al. DNA Repair and Mutagenesis 2nd edn 463–555 (ASM Press, 2006)
    Google Scholar
  4. Pham, P., Bertram, J. G., O'Donnell, M., Woodgate, R. & Goodman, M. F. A model for SOS-lesion-targeted mutations in Escherichia coli. Nature 409, 366–370 (2001)
    Article ADS CAS Google Scholar
  5. Becherel, O. J., Fuchs, R. P. & Wagner, J. Pivotal role of the β-clamp in translesion DNA synthesis and mutagenesis in E. coli cells. DNA Repair 1, 703–708 (2002)
    Article CAS Google Scholar
  6. Lenne-Samuel, N., Wagner, J., Etienne, H. & Fuchs, R. P. The processivity factor β controls DNA polymerase IV traffic during spontaneous mutagenesis and translesion synthesis in vivo. EMBO Rep. 3, 45–49 (2002)
    Article CAS Google Scholar
  7. Tang, M. et al. Roles of E. coli DNA polymerases IV and V in lesion-targeted and untargeted SOS mutagenesis. Nature 404, 1014–1018 (2000)
    Article ADS CAS Google Scholar
  8. Kato, T. & Shinoura, Y. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol. Gen. Genet. 156, 121–131 (1977)
    CAS PubMed Google Scholar
  9. Steinborn, G. Uvm mutants of Escherichia coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis. Mol. Gen. Genet. 165, 87–93 (1978)
    Article CAS Google Scholar
  10. Bruck, I., Woodgate, R., McEntee, K. & Goodman, M. F. Purification of a soluble UmuD′C complex from Escherichia coli: cooperative binding of UmuD′C to single-stranded DNA. J. Biol. Chem. 271, 10767–10774 (1996)
    Article CAS Google Scholar
  11. Woodgate, R., Rajagopalan, M., Lu, C. & Echols, H. UmuC mutagenesis protein of Escherichia coli: purification and interaction with UmuD and UmuD′. Proc. Natl Acad. Sci. USA 86, 7301–7305 (1989)
    Article ADS CAS Google Scholar
  12. Tang, M. et al. Biochemical basis of SOS-induced mutagenesis in Escherichia coli: reconstitution of in vitro lesion bypass dependent on the UmuD′2C mutagenic complex and RecA protein. Proc. Natl Acad. Sci. USA 95, 9755–9760 (1998)
    Article ADS CAS Google Scholar
  13. Fujii, S., Gasser, V. & Fuchs, R. P. The biochemical requirements of DNA polymerase V-mediated translesion synthesis revisited. J. Mol. Biol. 341, 405–417 (2004)
    Article CAS Google Scholar
  14. Nohmi, T., Battista, J. R., Dodson, L. A. & Walker, G. C. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proc. Natl Acad. Sci. USA 85, 1816–1820 (1988)
    Article ADS CAS Google Scholar
  15. Dutreix, M. et al. New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis. J. Bacteriol. 171, 2415–2423 (1989)
    Article CAS Google Scholar
  16. Sweasy, J. B., Witkin, E. M., Sinha, N. & Roegner-Maniscalco, V. RecA protein of Escherichia coli has a third essential role in SOS mutator activity. J. Bacteriol. 172, 3030–3036 (1990)
    Article CAS Google Scholar
  17. Kuzminov, A. Recombinational repair of DNA damage in Escherichia coli and bacteriophage λ. Microbiol. Mol. Biol. Rev. 63, 751–813 (1999)
    CAS PubMed PubMed Central Google Scholar
  18. Bridges, B. A. & Woodgate, R. Mutagenic repair in Escherichia coli. X. The umuC gene product may be required for replication past pyrimidine dimers but not for the coding error in UV-mutagenesis. Mol. Gen. Genet. 196, 364–366 (1984)
    Article CAS Google Scholar
  19. Echols, H. & Goodman, M. F. Mutation induced by DNA damage: a many protein affair. Mutat. Res. 236, 301–311 (1990)
    Article CAS Google Scholar
  20. Sommer, S., Boudsocq, F., Devoret, R. & Bailone, A. Specific RecA amino acid changes affect RecA–UmuD′C interaction. Mol. Microbiol. 28, 281–291 (1998)
    Article CAS Google Scholar
  21. Schlacher, K., Cox, M. M., Woodgate, R. & Goodman, M. F. RecA acts in trans to allow replication of damaged DNA by DNA polymerase V. Nature 442, 883–887 (2006)
    Article ADS CAS Google Scholar
  22. Eggler, A. L., Lusetti, S. L. & Cox, M. M. The C terminus of the Escherichia coli RecA protein modulates the DNA binding competition with single-stranded DNA-binding protein. J. Biol. Chem. 278, 16389–16396 (2003)
    Article CAS Google Scholar
  23. Dutreix, M., Burnett, B., Bailone, A., Radding, C. M. & Devoret, R. A partially deficient mutant, recA1730, that fails to form normal nucleoprotein filaments. Mol. Gen. Genet. 232, 489–497 (1992)
    Article CAS Google Scholar
  24. Wyatt, P. J. Light scattering and the absolute characterization of macromolecules. Anal. Chim. Acta 272, 1–40 (1993)
    Article CAS Google Scholar
  25. Schlacher, K. et al. DNA polymerase V and RecA protein, a minimal mutasome. Mol. Cell 17, 561–572 (2005)
    Article CAS Google Scholar

Download references