A genetically encoded photoactivatable Rac controls the motility of living cells (original) (raw)

References

  1. Goeldner, M. & Givens, R. Dynamic Studies in Biology: Phototriggers, Photoswitches and Caged Biomolecules (Wiley-VCH, 2005)
    Book Google Scholar
  2. Fortin, D. L. et al. Photochemical control of endogenous ion channels and cellular excitability. Nature Methods 5, 331–338 (2008)
    Article CAS Google Scholar
  3. Raftopoulou, M. & Hall, A. Cell migration: Rho GTPases lead the way. Dev. Biol. 265, 23–32 (2004)
    Article CAS Google Scholar
  4. Ridley, A. J. et al. Cell migration: integrating signals from front to back. Science 302, 1704–1709 (2003)
    Article ADS CAS Google Scholar
  5. Christie, J. M., Salomon, M., Nozue, K., Wada, M. & Briggs, W. R. LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. Proc. Natl Acad. Sci. USA 96, 8779–8783 (1999)
    Article ADS CAS Google Scholar
  6. Harper, S. M., Neil, L. C. & Gardner, K. H. Structural basis of a phototropin light switch. Science 301, 1541–1544 (2003)
    Article ADS CAS Google Scholar
  7. Kraynov, V. S. et al. Localized Rac activation dynamics visualized in living cells. Science 290, 333–337 (2000)
    Article ADS CAS Google Scholar
  8. Pertz, O., Hodgson, L., Klemke, R. L. & Hahn, K. M. Spatiotemporal dynamics of RhoA activity in migrating cells. Nature 440, 1069–1072 (2006)
    Article ADS CAS Google Scholar
  9. Burridge, K. & Wennerberg, K. Rho and Rac take center stage. Cell 116, 167–179 (2004)
    Article CAS Google Scholar
  10. Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D. & Hall, A. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 70, 401–410 (1992)
    Article CAS Google Scholar
  11. Sander, E. E., ten Klooster, J. P., van Delft, S., van der Kammen, R. A. & Collard, J. G. Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J. Cell Biol. 147, 1009–1022 (1999)
    Article CAS Google Scholar
  12. Yao, X., Rosen, M. K. & Gardner, K. H. Estimation of the available free energy in a LOV2-Jα photoswitch. Nature Chem. Biol. 4, 491–497 (2008)
    Article CAS Google Scholar
  13. Salomon, M., Christie, J. M., Knieb, E., Lempert, U. & Briggs, W. R. Photochemical and mutational analysis of the FMN-binding domains of the plant blue light receptor, phototropin. Biochemistry 39, 9401–9410 (2000)
    Article CAS Google Scholar
  14. Harper, S. M., Christie, J. M. & Gardner, K. H. Disruption of the LOV-Jα helix interaction activates phototropin kinase activity. Biochemistry 43, 16184–16192 (2004)
    Article CAS Google Scholar
  15. Thompson, G., Owen, D., Chalk, P. A. & Lowe, P. N. Delineation of the Cdc42/Rac-binding domain of p21-activated kinase. Biochemistry 37, 7885–7891 (1998)
    Article CAS Google Scholar
  16. Patterson, G. H. & Lippincott-Schwartz, J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297, 1873–1877 (2002)
    Article ADS CAS Google Scholar
  17. Vicente-Manzanares, M., Zareno, J., Whitmore, L., Choi, C. K. & Horwitz, A. F. Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells. J. Cell Biol. 176, 573 (2007)
    Article CAS Google Scholar
  18. Burridge, K. & Chrzanowska-Wodnicka, M. Focal adhesions, contractility, and signaling. Annu. Rev. Cell Dev. Biol. 12, 463–518 (1996)
    Article CAS Google Scholar
  19. Giannone, G. et al. Lamellipodial actin mechanically links myosin activity with adhesion-site formation. Cell 128, 561–575 (2007)
    Article CAS Google Scholar
  20. Davies, S. P., Reddy, H., Caivano, M. & Cohen, P. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem. J. 351, 95–105 (2000)
    Article CAS Google Scholar
  21. Kurokawa, K. & Matsuda, M. Localized RhoA activation as a requirement for the induction of membrane ruffling. Mol. Biol. Cell 16, 4294–4303 (2005)
    Article CAS Google Scholar
  22. Rohl, C. A., Strauss, C. E., Misura, K. M. & Baker, D. Protein structure prediction using Rosetta. Methods Enzymol. 383, 66–93 (2004)
    Article CAS Google Scholar
  23. Halavaty, A. S. & Moffat, K. N- and C-terminal flanking regions modulate light-induced signal transduction in the LOV2 domain of the blue light sensor phototropin 1 from Avena sativa. Biochemistry 46, 14001–14009 (2007)
    Article CAS Google Scholar
  24. Nobes, C. D. & Hall, A. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81, 53–62 (1995)
    Article CAS Google Scholar
  25. Lee, J. et al. Surface sites for engineering allosteric control in proteins. Science 322, 438–442 (2008)
    Article ADS CAS Google Scholar
  26. Moglich, A., Ayers, R. A. & Moffat, K. Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Biol. 385, 1433–1444 (2008)
    Article Google Scholar
  27. Strickland, D., Moffat, K. & Sosnick, T. R. Light-activated DNA binding in a designed allosteric protein. Proc. Natl Acad. Sci. USA 105, 10709–10714 (2008)
    Article ADS CAS Google Scholar
  28. Leung, D. W., Otomo, C., Chory, J. & Rosen, M. K. Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway. Proc. Natl Acad. Sci. USA 105, 12797–12802 (2008)
    Article ADS CAS Google Scholar
  29. Hodgson, L., Shen, F. & Hahn, K. M. Biosensors for characterizing the dynamics of Rho family GTPases in living cells. Curr. Protoc. Cell Biol. (in the press)

Download references