Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate (original) (raw)

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Acknowledgements

We thank K. Kainulainen, M. Herpola, G.-B. Berglund and J. Jung for technical assistance; M. Prigge, S. Clark, C. Bellini, N. Sauer, J. Colinas, T. Vernoux, K. Gallagher, A. P. Mahonen, A. Bishopp, M. Bonke, N. Fedoroff, J. C. Fletcher, B. J. Reinhart, I. Pekker, ABRC and NASC for materials, and E. Richards and M. Harrison for comments on the manuscript; M. Tsiantis for sharing results before publication. This work was supported by the Boyce Thompson Institute and NSF IOS0818071 to J.-Y.L., Cornell Presidential Life Science Fellowship to J.Z., grants from the NIH (RO1-GM043778) and from the NSF ARABIDOPSIS 2010 programme to P.N.B., a fellowship from the MICINN, Spanish Government to M.A.M.-R., grants by the Academy of Finland, Tekes and ESF to Y.H., S.L. and A.V., European Molecular Biology Organisation (EMBO, ALTF 450-2007) to J.D., Estonian funding agencies (ETF7361 and SF0180071s07) to O.L., Nilsson-Ehle Foundation to C.J.R, and FORMAS and Carl Trygger’s Foundation for Scientific Research to A.C. Imaging at Boyce Thompson Institute was supported by NSF (NSF DBI-0618969) and Triad Foundation.

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Author notes

  1. Annelie Carlsbecker, Ji-Young Lee, Ykä Helariutta and Philip N. Benfey: These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Biotechnology/Department of Biosciences, University of Helsinki, FIN-00014, Finland
    Annelie Carlsbecker, Jan Dettmer, Satu Lehesranta, Ove Lindgren, Anne Vatén, Siripong Thitamadee, Ana Campilho & Ykä Helariutta
  2. Department of Physiological Botany, Evolutionary Biology Center, Uppsala University, Norbyvägen 18D, SE-752 36 Uppsala, Sweden,
    Annelie Carlsbecker & Christina J. Roberts
  3. Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, New York 14853, USA ,
    Ji-Young Lee, Jing Zhou & Jose Sebastian
  4. Graduate Field of Plant Biology, Cornell University, Ithaca, New York 14853, USA ,
    Ji-Young Lee & Jing Zhou
  5. Institute of Technology, University of Tartu, Tartu 50411, Estonia
    Ove Lindgren
  6. Biology Department and IGSP Center for Systems Biology, Duke University, Durham, North Carolina 27708, USA,
    Miguel A. Moreno-Risueno & Philip N. Benfey
  7. School of Biological Sciences, Monash University, Melbourne, Victoria 3800, Australia ,
    John L. Bowman

Authors

  1. Annelie Carlsbecker
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  2. Ji-Young Lee
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  3. Christina J. Roberts
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  4. Jan Dettmer
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  5. Satu Lehesranta
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  6. Jing Zhou
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  7. Ove Lindgren
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  8. Miguel A. Moreno-Risueno
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  9. Anne Vatén
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  10. Siripong Thitamadee
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  11. Ana Campilho
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  12. Jose Sebastian
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  13. John L. Bowman
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  14. Ykä Helariutta
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  15. Philip N. Benfey
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Contributions

A.C. and J.-Y.L. contributed equally to this work, C.J.R., J.D., S.L. and J.Z. contributed equally to this work, O.L., M.A.M.-R. and A.V. contributed equally to this work, Y.H. and P.N.B. contributed equally to this work, and the name order was determined by raffle. A.C. designed and performed experiments to characterize HD-ZIP III transcription factors and miR165/6 in vascular patterning, J.-Y.L. identified and characterized the regulatory network of SHR, SCR and miR165/6 in the xylem patterning, C.J.R. analysed the miRNA expression by in situ hybridization and participated in HD-ZIP III mutant characterization. J.D. participated in the analysis of expression of PHB (including mutant forms) and other HD-ZIP III and generated pCRE1::MIR165a as well as participated in the generation of J0571 lines to rescue scr and shr. S.L. participated in the PHB and HD-ZIP III expression analysis, phb-7d mutant characterization and generated the pSCR::MIR165a to rescue scr. J.Z. developed and characterized the xylem patterning led by non-mobile SHR and PHB-m. O.L. participated in the characterization of various HD-ZIP III mutant lines. M.A.M.-R. showed the non-cell autonomous action of non-mobile SHR. A.V. participated in positional cloning of phb-7d and establishment of the J0571 lines to rescue shr. S.T. identified the phb-7d mutant. A.C. identified the scr-6 allele. J.S. characterized shr/scr and HD-ZIP III double mutants and embryo expression patterns in GFP lines. J.L.B. shared informative non-published materials. Y.H. and P.N.B. participated in experimental design. A.C., J.-Y.L., Y.H. and P.N.B. wrote the manuscript. A.C. and J.-Y.L. are co-corresponding authors. All authors discussed the results and commented on the manuscript.

Corresponding authors

Correspondence toYkä Helariutta or Philip N. Benfey.

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Carlsbecker, A., Lee, JY., Roberts, C. et al. Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate.Nature 465, 316–321 (2010). https://doi.org/10.1038/nature08977

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