An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis (original) (raw)

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Gene Expression Omnibus

Data deposits

All microarray data are deposited in GEO under accession numbers GSE19491, GSE19444, GSE19443, GSE19442, GSE19439, GSE19435 and GSE 22098. Some of the work has been submitted as US patent application PCT 371: Blood Transcriptional Signature of Mycobacterium Tuberculosis Infection: Serial No: 12/602,488.

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Acknowledgements

We thank the patients and volunteer participants. We thank D. Kioussis (MRC National Institute for Medical Research (NIMR)) and D. Young (NIMR) for discussion and input. We thank N. Baldwin (Baylor Institute for Immunology Research (BIIR)) for advice and support on bioinformatics analysis, Q.-A. Nguyen (BIIR) and colleagues for providing technical assistance with microarray processing, and S. Caidan (NIMR), J. Wills (NIMR) and S. Phillips (BIIR) for help and advice with sample storage and transport. We thank the TB service at Imperial College Healthcare NHS Trust, B.M. Haselden and the TB service at Hillingdon Hospital, Uxbridge, UK. We also thank H. Giedon and R. Seldon for help in laboratory analyses, and Y. Hlombe for recruitment of patients and follow-up in South Africa. A. Rae (NIMR), T. Dipucchio (BIIR) and K. Palucka (BIIR) provided advice on flow cytometry. We thank G. Hayward for help depositing the microarray data. We thank J. Brock (NIMR) for help with graphics. M.P.R.B. was supported by an MRC career development fellowship and a grant from the Dana Foundation Program in Human Immunology. The research was funded by the Medical Research Council, UK, MRC Grant U117565642 and The Dana Foundation Program in Human Immunology. A.O’G., C.M.G. and F.W.McN. are funded by the Medical Research Council, UK. V.P. is supported by National Institutes of Health (NIH) R01 AR050770-01, NIH P50 ARO54083 and NIH 1 U19 AI082715-01. The work of J.B., D.C. and V.P. is supported by the Baylor Health Care System Foundation and the NIH (U19 AIO57234-02, U01 AI082110, P01 CA084512).

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Author notes

1. Christine M. Graham and Finlay W. McNab: These authors contributed equally to this work.

Authors and Affiliations

1. Division of Immunoregulation, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK,
   Matthew P. R. Berry, Christine M. Graham, Finlay W. McNab & Anne O’Garra
2. Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK,
   Katalin A. Wilkinson & Robert J. Wilkinson
3. Department of Respiratory Medicine, St. Mary’s Hospital, Imperial College Healthcare NHS Trust, London, W2 1NY, UK
   Susannah A. A. Bloch & Onn M. Kon
4. Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, 7925, South Africa
   Tolu Oni, Katalin A. Wilkinson & Robert J. Wilkinson
5. Division of Medicine, Wright Fleming Institute, Imperial College, London, W2 1PG, UK
   Tolu Oni & Robert J. Wilkinson
6. Baylor Institute for Immunology Research/ANRS Center for Human Vaccines, INSERM U899, 3434 Live Oak Street, Dallas, Texas 75204, USA,
   Zhaohui Xu, Jason Skinner, Charles Quinn, John J. Cush, Virginia Pascual, Jacques Banchereau & Damien Chaussabel
7. Institute for Health Care Research and Improvement, Baylor Health Care System, Dallas, 75206, Texas, USA
   Derek Blankenship
8. Department of Radiology, St Mary’s Hospital, Imperial College Healthcare NHS Trust, London, W2 1NY, UK
   Ranju Dhawan
9. UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA,
   Romain Banchereau
10. Center for Vaccines and Immunity, The Research Institute at Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, Ohio 43205, USA,
    Asuncion Mejias & Octavio Ramilo

Authors

1. Matthew P. R. Berry
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2. Christine M. Graham
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3. Finlay W. McNab
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4. Zhaohui Xu
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5. Susannah A. A. Bloch
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6. Tolu Oni
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7. Katalin A. Wilkinson
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8. Romain Banchereau
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9. Jason Skinner
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10. Robert J. Wilkinson
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11. Charles Quinn
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12. Derek Blankenship
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13. Ranju Dhawan
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14. John J. Cush
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15. Asuncion Mejias
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16. Octavio Ramilo
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17. Onn M. Kon
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18. Virginia Pascual
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19. Jacques Banchereau
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20. Damien Chaussabel
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21. Anne O’Garra
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Contributions

M.P.R.B., D.C., O.M.K and A.O’G. designed the study on TB with input from J.B. and R.J.W. and for other diseases with input from V.P. and O.R.; M.P.R.B., S.A.A.B., T.O., K.A.W., J.J.C., A.M., R.B. and O.M.K. recruited, sampled and collected data about patients; M.P.R.B., R.B., A.M. and C.M.G. processed whole blood for microarray experiments with help from J.S.; C.G. performed blood-cell subset separations and processing for microarray experiments with help from J.S.; M.P.R.B., C.M.G. and Z.X. performed microarray data analysis, with advice and input from J.S., D.C. and V.P.; M.P.R.B. and Z.X. performed Ingenuity, modular and ‘molecular distance to health’ analyses; M.P.R.B. performed multiplex serum analyses; F.W.McN. performed flow cytometry analysis; D.C., V.P. and A.O’G. supervised data analysis; M.P.R.B. and D.B. performed statistical analysis; M.P.R.B., S.A.A.B., R.D. and O.M.K performed analyses of radiology; A.O’G. and M.P.R.B. wrote the manuscript, with early input from C.M.G., F.W.McN., J.B., D.C. and J.S., and subsequently all authors provided advice and approved the final manuscript.

Corresponding author

Correspondence toAnne O’Garra.

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The authors declare no competing financial interests.

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Berry, M., Graham, C., McNab, F. et al. An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis.Nature 466, 973–977 (2010). https://doi.org/10.1038/nature09247

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• Received: 23 March 2010
• Accepted: 02 June 2010
• Issue Date: 19 August 2010
• DOI: https://doi.org/10.1038/nature09247