A conditional knockout resource for the genome-wide study of mouse gene function (original) (raw)

• Gossler, A., Joyner, A. L., Rossant, J. & Skarnes, W. C. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244, 463–465 (1989)
  Article CAS ADS Google Scholar
• Skarnes, W. C., Auerbach, B. A. & Joyner, A. L. A gene trap approach in mouse embryonic stem cells: the lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev. 6, 903–918 (1992)
  Article CAS Google Scholar
• Zambrowicz B. P et al. Wnk1 kinase deficiency lowers blood pressure in mice: a gene-trap screen to identify potential targets for therapeutic intervention. Proc. Natl Acad. Sci. USA 100, 14109–14114 (2003)
  Article ADS Google Scholar
• International Gene Trap Consortium. A public gene trap resource for mouse functional genomics. Nature Genet. 36, 543–544 (2004)
• Hansen, G. M. et al. Large-scale gene trapping in C57BL/6N mouse embryonic stem cells. Genome Res. 18, 1670–1679 (2008)
  Article CAS Google Scholar
• Bradley, A., Evans, M., Kaufman, M. H. & Robertson, E. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature 309, 255–256 (1984)
  Article CAS ADS Google Scholar
• Robertson, E., Bradley, A., Kuehn, M. & Evans, M. Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature 323, 445–448 (1986)
  Article CAS ADS Google Scholar
• Thomas, K. R. & Capecchi, M. R. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51, 503–512 (1987)
  Article CAS Google Scholar
• van der Weyden, L., Adams, D. J. & Bradley, A. Tools for targeted manipulation of the mouse genome. Physiol. Genomics 11, 133–164 (2002)
  Article CAS Google Scholar
• Glaser, S., Anastasiadis, K. & Stewart, A. F. Current issues in mouse genome engineering. Nature Genet. 37, 1187–1193 (2005)
  Article CAS Google Scholar
• Valenzuela, D. M. et al. High-throughput engineering of the mouse genome coupled with high-resolution expression analysis. Nature Biotechnol. 21, 652–659 (2003)
  Article CAS Google Scholar
• Lettice, L. A. et al. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly. Proc. Natl Acad. Sci. USA 99, 7548–7553 (2002)
  Article CAS ADS Google Scholar
• Zuniga, A. et al. Mouse limb deformity mutations disrupt a global control region within the large regulatory landscape required for Gremlin expression. Genes Dev. 18, 1553–1564 (2004)
  Article CAS Google Scholar
• International Mouse Knockout Consortium . A mouse for all reasons. Cell 128, 9–13 (2007)
  Article Google Scholar
• Poueymirou, W. T. et al. F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses. Nature Biotechnol. 25, 91–99 (2007)
  Article CAS Google Scholar
• Pettitt, S. J. et al. Agouti C57BL/6N embryonic stem cells for mouse genetics resources. Nature Methods 6, 493–495 (2009)
  Article CAS Google Scholar
• Gertsenstein, M. et al. Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos. PLoS ONE 5, e11260 (2010)
  Article ADS Google Scholar
• Ringwald, M. et al. The IKMC web portal: a central point of entry to data and resources from the International Knockout Mouse Consortium. Nucleic Acids Res. 39 (Database issue). D849–D855 (2011)
  Article CAS Google Scholar
• Branda, C. S. & Dymecki, S. M. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev. Cell 6, 7–28 (2004)
  Article CAS Google Scholar
• Testa, G. et al. A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. Genesis 38, 151–158 (2004)
  Article CAS Google Scholar
• Mitchell, K. J. et al. Functional analysis of secreted and transmembrane proteins critical to mouse development. Nature Genet. 28, 241–249 (2001)
  Article CAS Google Scholar
• Yang, S. H. et al. Caution! Analyze transcripts from conditional knockout alleles. Transgenic Res. 18, 483–489 (2009)
  Article CAS Google Scholar
• Liang, Q., Conte, N., Skarnes, W. C. & Bradley, A. Extensive genomic copy number variation in embryonic stem cells. Proc. Natl Acad. Sci. USA 105, 17453–17456 (2008)
  Article CAS ADS Google Scholar
• Wilming, L. G. et al. The vertebrate genome annotation (Vega) database. Nucleic Acids Res. 36, D753–D760 (2008)
  Article CAS Google Scholar
• Sarov, M. et al. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nature Methods 3, 839–844 (2006)
  Article CAS Google Scholar
• Poser, I. et al. BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nature Methods 5, 409–415 (2008)
  Article CAS ADS Google Scholar
• Osoegawa, K. et al. Bacterial artificial chromosome libraries for mouse sequencing and functional analysis. Genome Res. 10, 116–128 (2000)
  CAS PubMed PubMed Central Google Scholar
• Hope, I. A. et al. Feasibility of genome-scale construction of promoter:reporter gene fusions for expression in Caenorhabditis elegans using a multisite gateway recombination system. Genome Res. 14, 2070–2075 (2004)
  Article CAS Google Scholar
• Rual, J. F. et al. Towards a proteome-scale map of the human protein–protein interaction network. Nature 437, 1173–1178 (2005)
  Article CAS ADS Google Scholar
• Mansour, S. L., Thomas, K. R. & Capecchi, M. R. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 336, 348–352 (1988)
  Article CAS ADS Google Scholar
• Yagi, T. et al. Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection. Proc. Natl Acad. Sci. USA 87, 9918–9922 (1990)
  Article CAS ADS Google Scholar
• Lay, J. M., Friis-Hansen, L., Gillespie, P. J. & Samuelson, L. C. Rapid confirmation of gene targeting in embryonic stem cells using two long-range PCR techniques. Transgenic Res. 7, 135–140 (1998)
  Article CAS Google Scholar
• Friedel, R. H. et al. Gene targeting using a promoterless gene trap vector (“targeted trapping”) is an efficient method to mutate a large fraction of genes. Proc. Natl Acad. Sci. USA 102, 13188–13193 (2005)
  Article CAS ADS Google Scholar
• Skarnes, W. C. Two ways to trap a gene in mice. Proc. Natl Acad. Sci. USA 102, 13001–13002 (2005)
  Article CAS ADS Google Scholar
• Skarnes, W. C., Moss, J. E., Hurtley, S. M. & Beddington, R. S. Capturing genes encoding membrane and secreted proteins important for mouse development. Proc. Natl Acad. Sci. USA 92, 6592–6596 (1995)
  Article CAS ADS Google Scholar
• de Felipe, P., Luke, G. A., Brown, J. D. & Ryan, M. D. Inhibition of 2A-mediated ‘cleavage’ of certain artificial polyproteins bearing N-terminal signal sequences. Biotechnol. J. 5, 213–223 (2010)
  Article CAS Google Scholar
• Hasty, P., Rivera-Perez, J. & Bradley, A. The length of homology required for gene targeting in embryonic stem cells. Mol. Cell. Biol. 11, 5586–5591 (1991)
  Article CAS Google Scholar
• Deng, C. & Capecchi, M. R. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol. Cell. Biol. 12, 3365–3371 (1992)
  Article CAS Google Scholar
• te Riele, H., Maanday, E. R. & Berns, A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl Acad. Sci. USA 89, 5128–5132 (1992)
  Article CAS ADS Google Scholar
• Yanagawa, Y. et al. Enrichment and efficient screening of ES cells containing a targeted mutation: the use of DT-A gene with the polyadenylation signal as a negative selection maker. Transgenic Res. 8, 215–221 (1999)
  Article CAS Google Scholar
• Collins, S. R. et al. Toward a comprehensive atlas of the physical interactome of Saccharomyces cerevisiae. Mol. Cell. Proteomics 6, 439–450 (2007)
  Article CAS Google Scholar
• Buehr, M. et al. Capture of authentic embryonic stem cells from rat blastocysts. Cell 135, 1287–1298 (2008)
  Article CAS Google Scholar
• Li, P. et al. Germline competent embryonic stem cells derived from rat blastocysts. Cell 135, 1299–1310 (2008)
  Article CAS Google Scholar
• Thomson, J. A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147 (1998)
  Article CAS ADS Google Scholar
• Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861–872 (2007)
  Article CAS Google Scholar
• Bozdech, Z. et al. Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray. Genome Biol. 4, R9 (2003)
  Article Google Scholar
• Wang, J. et al. An improved recombineering approach by adding RecA to lambda Red recombination. Mol. Biotechnol. 32, 43–53 (2006)
  Article Google Scholar
• Kast, P. pKSS—a second-generation general purpose cloning vector for efficient positive selection of recombinant clones. Gene 138, 109–114 (1994)
  Article CAS Google Scholar