RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis (original) (raw)

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Nature 487, 510–513 (2012); doi:10.1038/nature11217 In this Letter, we omitted the following accession information. The digital gene expression matrix and the Helicos single-molecule sequence data from which it was derived are available at the NCBI GEO database with accession number GSE40176. A total of 44 samples were uploaded including 12 mouse and 32 human samples.

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Acknowledgements

We thank C. Koris and the MGH clinical research coordinators for help with clinical studies, J. Gentry for computer informatics support, L. Libby for mouse studies, J. Walsh for microscopy expertise, Z. Nakamura for technical support, D. Jones for sequencing, and M. Rivera and S. Akhavanfard for RNA-ISH. This work was supported by Stand Up To Cancer (D.A.H., M.T., S.M.), Howard Hughes Medical Institute (D.A.H.), NIBIB Quantum Grant 5R01EB008047 (M.T.), NIH CA129933 (D.A.H.), Pancreatic Cancer Action Network – AACR Fellowship (D.T.T.), the Warshaw Institute for Pancreatic Cancer Research (D.T.T.), and the K12 Paul Calabresi Award for Clinical Oncology Clinical Research Career Development Program NIH 5K12CA87723-09 (D.T.T.).

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Author notes

  1. Min Yu and David T. Ting: These authors contributed equally to this work.

Authors and Affiliations

  1. and Departments of Medicine and Surgery, Massachusetts General Hospital Cancer Center, Center for Engineering in Medicine, Harvard Medical School, Boston, 02114, Massachusetts, USA
    Min Yu, David T. Ting, Shannon L. Stott, Ben S. Wittner, Suchismita Paul, Jordan C. Ciciliano, Malgorzata E. Smas, Daniel Winokur, Anna J. Gilman, Matthew J. Ulman, Kristina Xega, Gianmarco Contino, Brinda Alagesan, Brian W. Brannigan, David P. Ryan, Lecia V. Sequist, Nabeel Bardeesy, Sridhar Ramaswamy, Mehmet Toner, Shyamala Maheswaran & Daniel A. Haber
  2. Howard Hughes Medical Institute, Chevy Chase, 20815, Maryland, USA
    Min Yu & Daniel A. Haber
  3. Helicos BioSciences Corporation, One Kendall Square, Cambridge, 02139, Massachusetts, USA
    Fatih Ozsolak & Patrice M. Milos

Authors

  1. Min Yu
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  2. David T. Ting
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  3. Shannon L. Stott
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  4. Ben S. Wittner
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  5. Fatih Ozsolak
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  6. Suchismita Paul
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  7. Jordan C. Ciciliano
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  8. Malgorzata E. Smas
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  9. Daniel Winokur
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  10. Anna J. Gilman
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  11. Matthew J. Ulman
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  12. Kristina Xega
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  13. Gianmarco Contino
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  14. Brinda Alagesan
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  15. Brian W. Brannigan
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  16. Patrice M. Milos
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  17. David P. Ryan
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  18. Lecia V. Sequist
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  19. Nabeel Bardeesy
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  20. Sridhar Ramaswamy
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  21. Mehmet Toner
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  22. Shyamala Maheswaran
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  23. Daniel A. Haber
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Contributions

M.Y., D.T.T., S.M. and D.A.H. designed and conducted the study, analysed data, and prepared the manuscript. S.L.S. and M.T. contributed to the microfluidic system. D.T.T., B.S.W., F.O., B.W.B. and P.M.M. performed Helicos digital gene expression analysis. B.S.W. and S.R. performed bioinformatic and statistical analysis. S.P., J.C.C., M.E.S., D.W., A.J.G., M.J.U. and K.X. provided technical assistance for microfluidic experiments. M.Y., D.T.T., B.W.B. and J.C.C. performed RNA-ISH analysis. D.T.T., D.P.R. and L.V.S. acquired clinical samples. G.C., B.A. and N.B. provided the mouse model and cell line. S.L.S., B.S.W., P.M.M., S.R. and M.T. commented on the manuscript.

Corresponding authors

Correspondence toShyamala Maheswaran or Daniel A. Haber.

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The authors declare no competing financial interests.

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Yu, M., Ting, D., Stott, S. et al. RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.Nature 487, 510–513 (2012). https://doi.org/10.1038/nature11217

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