Anti-diabetic activity of insulin-degrading enzyme inhibitors mediated by multiple hormones (original) (raw)
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Protein Data Bank
Data deposits
The coordinates and the structure factors of the IDE·6b complex have been deposited in the Protein Data Bank under the accession code 4LTE.
References
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Acknowledgements
This research was supported by NIH/NIGMS (R01 GM065865 (D.R.L.), R00 GM080097 (M.A.S.), R01 GM81539 (W.-J.T.), T32 GM008444 (Z.H.F.), F30 CA174152 (Z.H.F.), DP2 OD002374 (A.S.)), Howard Hughes Medical Institute (D.R.L.), Diabetes and Cancer Centers of Albert Einstein College of Medicine (M.J.C.), American Diabetes Association no. 7-11-CD-06 (M.A.L.), Burroughs Wellcome Fund CABS (A.S.), and the Searle Scholars Program (A.S.). The Fonds de Recherche en Santé du Québec (FRSQ) and the Alfred Bader Fund supported J.P.M. We thank C. Russ and H. Spurling (Broad Institute) and C. Daly (FAS Center for Systems Biology) for DNA sequencing assistance. We are grateful to S. Johnston and C. Mosher (Broad Institute) for 6bK stability measurements. W. Nolte provided mouse IDE, L. McCord purified CF-IDE and Y.-G. Kim performed CGRP cleavage assays. We are grateful to A. Badran, E. Homan, A. M. Lone and M. Leidl (Harvard University) for experimental assistance. We thank B. Kahn and N. Gray for discussions.
Author information
Authors and Affiliations
- Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, 02138, Massachusetts, USA
Juan Pablo Maianti, Amanda McFedries, Ralph E. Kleiner, Alan Saghatelian & David R. Liu - Department of Pharmacological Sciences, Stony Brook University, 1 Circle Road, Stony Brook, 11794, New York, USA
Zachariah H. Foda & Markus A. Seeliger - Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, 10461, New York, USA
Xiu Quan Du & Maureen J. Charron - Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, 3204 Biological Sciences III, Irvine, 92697, California, USA
Malcolm A. Leissring - Ben-May Department for Cancer Research, University of Chicago, 929 East 57th Street, Chicago, 60637, Illinois, USA
Wei-Jen Tang - Department of Chemistry and Chemical Biology, Howard Hughes Medical Institute, Harvard University, 12 Oxford Street, Cambridge, 02138, Massachusetts, USA
David R. Liu
Authors
- Juan Pablo Maianti
You can also search for this author inPubMed Google Scholar - Amanda McFedries
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Contributions
J.P.M., A.M., Z.H.F., R.E.K. and X.Q.D. performed the experiments. M.A.L. provided the _Ide_–/– mice. W.-J.T. provided IDE protein for structural studies. M.J.C. supervised the _Gcgr_–/– studies. M.A.S. supervised the IDE·6b structural studies. A.S. supervised the pharmacological validation of 6bK and the in vivo studies. D.R.L. supervised the discovery of IDE inhibitors, the pharmacological studies and the in vivo studies. All authors analysed the data and wrote the manuscript.
Corresponding authors
Correspondence toAlan Saghatelian or David R. Liu.
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Competing interests
J.P.M., A.S. and D.R.L. are co-inventors on a provisional patent application that describes the discovery and properties of 6bK.
Extended data figures and tables
Extended Data Figure 1
a, Enrichment plots from two independent in vitro selections against N-His6-mIDE using a 13,824-membered DNA-templated macrocycle library7,8. The numbers highlight compounds enriched at least twofold in both selections. b, Structures of IDE-binding macrocycles 1–6 decoded from DNA library barcodes corresponding to building blocks A, B, C and D (Fig. 1). The cis and trans isomers are indicated by suffices a and b respectively (for example, 1a and 1b). The two isomers were synthesized as previously reported18,19 and separated by HPLC. c, IDE inhibition potency of trans hits 1b to 6b compared to 30 structurally related 6b analogues in which the linker, scaffold and the three building blocks were systematically varied. IDE inhibition activity was assayed by following cleavage of the fluorogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH.
Extended Data Figure 2 Inhibition of human and mouse IDE activity in multiple assays.
a, b, Human IDE shares 95% sequence homology with mouse IDE13, and cleavage of the fluorogenic substrate peptide Mca-RPPGFSAFK(Dnp)-OH by human and mouse IDE is inhibited with similar potency by 6b (a) and 6bK (b). c, Homogeneous time-resolved fluorescence (HTRF) assay measuring degradation of insulin by IDE in the presence of 6b, 6bK and analogue 28 (Extended Data Fig. 1). d, LC-MS assay for ex vivo degradation of CGRP (10 µM) by endogenous IDE in mouse plasma in the presence of 6b. e, f, Biochemical assays suggesting that 6b binds a site in IDE distinct from the conventional peptide substrate binding site known to bind substrate mimetic Ii1. Yonetani–Theorell double inhibitor plots of IDE activity in the presence of 6b and Ii1 (panel e), or 6b and bacitracin (panel f). Crossing lines indicate synergistic and independent binding of inhibitors, while parallel lines indicate competition for binding to the enzyme.
Extended Data Figure 3 Data collection and refinement statistics (molecular replacement), docking simulation for 6b, and competition test between insulin and fluorescein-labelled macrocycle 31 for binding cysteine-free IDE (CF-IDE).
a, One crystal was used to solve the CF-IDĖ6b structure. The highest-resolution shell is shown in parentheses. Structure coordinates are deposited in the Protein Data Bank (accession number 4LTE). b, Molecular docking simulations are consistent with the placement of building blocks A and B in the structural model (two views shown: top and bottom panels). The structure of 6b in the binding site from crystallographic data with composite omit map contoured at 1.0_σ_ (_p_-benzoyl-phenylalanine is shown in red, the cyclohexylalanine in blue, the fumarate linker in grey, and the d-lysine backbone in purple). c, Highest-scoring pose from DOCK simulations (glutamine group is shown in green, see Supplementary Information for docking calculations). d, Structure of macrocycle 6b and fluorescent analogue 31. Stars indicate atoms not resolved in the crystal structure (the Gln building block and four atoms of the flexible macrocycle backbone). e, Competition test between the fluorescein-labelled macrocycle 31 and insulin for binding CF-IDE. Cysteine-free catalytically inactive IDE was titrated against 0.9 nM macrocycle 31 alone (filled green circles) or together with 2.15 μM insulin (red filled circles), producing a shift in apparent dissociation constant for macrocycle 31 according to equation (1) (Supplementary Information). f, Residue-decomposed energy of the crystal (green) and docked (blue) poses of 6b (see Supplementary Information for docking calculations).
Extended Data Figure 4 Small-molecule/enzyme mutant complementation study to confirm the macrocycle binding site and placement of the benzophenone and cyclohexyl building-block groups.
a, IDE mutant A479L is inhibited by 6b >600-fold less potently compared to wild-type IDE. b, Analogue 9, in which the _p_-benzoyl ring is substituted for a smaller _t_-butyl group, inhibits A479L IDE and WT IDE comparably. c, Similarly, IDE mutant G362Q is inhibited 77-fold less potently by 6b compared with WT IDE. d, Analogue 13, in which the L-cyclohexyl alanine side chain was substituted with a smaller L-leucine side chain, inhibits G362Q IDE and WT IDE comparably. The full list of IDE mutants investigated is shown in Supplementary Table 5. e, Synthetic scheme for 6bK, also used for synthesis of 6bK analogues.
Extended Data Figure 5 Pharmacokinetic parameters of 6bK, augmented insulin hypoglycaemic action by 6bK in mice, and effects of 6bK on amyloid peptide levels.
a, Plasma binding, plasma stability, and microsomal stability (1 h incubation) data (S. Johnston and C. Mosher, personal communication). b, Heavy-labelled 6bK was synthesized with 15N,13C-lysine for stable-isotope dilution LC-MS quantitation. c, Behaviour and post-experiment body weight measurements were not affected for mice treated with 6bK (green; 80 mg kg−1) versus vehicle alone (black). The mice were active, and displayed normal posture, normal grooming and normal response to stimulation. d, Concentration of 6bK in mice tissues and plasma collected over 4 h determined by isotope dilution mass spectrometry (IDMS) with heavy-labelled analogue (n = 2 for all data except the last two time-points, for which n = 1). e, Average biodistribution of 6bK in five lean mice at 150 min post-injection of 6bK 80 mg kg−1 i.p. at the endpoint of an IPGTT experiment. We did not detect 6bK in the brain even using tenfold concentrated samples for LC-MS injection compared to other tissues. f, Treatment of C57BL/6J lean mice with 6bK (green, 80 mg kg−1, n = 6) does not change brain levels of Aβ(40) or Aβ(42) peptides in the brain 2 h post injection compared to treatment with vehicle alone (black, n = 5) or inactive diastereomer bisepi-6bK (brown, 80 mg kg−1, n = 6). g, Mice treated with a single injection of 6bK (green, 80 mg kg−1 i.p.) display increased hypoglycaemic response to insulin injection (Humulin-R, 1.0 U kg−1 i.p., see also Fig. 3b). Data shown in c–e and g are representative of two or more independent studies.
Extended Data Figure 6 Dependence of insulin and glucagon secretion on the route of glucose administration (oral or i.p.) due to both the ‘incretin effect’ and the hyperinsulinaemic phenotype of DIO versus lean mice.
a, The early insulin response to glucose in lean and DIO mice is higher during OGTT than IPGTT. b, Suppression of glucagon secretion post-glucose administration is less effective after IPGTT and in DIO mice. c, Administration of 6bK (green, 80 mg kg−1, n = 7) to lean mice not followed by injection of a nutrient such as glucose or pyruvate (see Figs 2–3) did not significantly alter basal blood glucose or basal hormone levels compared to bisepi-6bK (brown, n = 7) or vehicle controls (black, n = 7) 30 min post-injection. All data points and error bars represent mean ± s.e.m. Statistics were performed using a two-tail Student’s _t_-test, and significance levels shown in the figures are *P < 0.05 or **P < 0.01 between the labelled groups. Data shown are representative of two or more independent studies.
Extended Data Figure 7 Low-potency diastereomers of 6bK used to determine effective dose range and confirm on-target IDE inhibition effects during IPGTTs.
a, Left, inhibition of mouse IDE activity by low potency diastereomers of 6bK (right). The stereocentres altered in each compound relative to those of 6bK are labelled with asterisks. b–e, In dose optimization experiments, the effects of 6bK (40 to 90 mg kg−1, n = 5 and 4, respectively) were compared with equal doses of either weakly active stereoisomer epi-C-6bK (n = 5, panel b) or inactive stereoisomer bisepi-6bK (n = 5, panel c) and vehicle controls (n = 5) in IPGTTs using lean and obese mice. d, DIO mice treated with low doses of 6bK (40 mg kg−1, n = 7) responded to IPGTT in either of two ways: improved glucose tolerance throughout the experiment (n = 3) or a hyperglycaemic ‘rebound’ as described in the main text (n = 4), suggesting this dose is too low to achieve a consistent ‘rebound’ phenotype (note the large error bars). e, DIO mice treated with high doses of 6bK (3.5 mg per animal, 90 mg kg−1, n = 5) respond similarly to Fig. 2e (2 mg per animal, 60 mg kg−1), but the weak activity observed for bisepi-6bK (IC50 > 200 µM) using a matching dose (90 mg kg−1, n = 5) compared to vehicle alone (n = 5) suggests that 60 mg kg−1 (2 mg per animal) is the appropriate dose for DIO mice experiments. All data points and error bars represent mean ± s.e.m. Statistics were performed using a two-tail Student’s _t_-test, and significance levels shown in the figures are *P < 0.05 versus vehicle control group; **P < 0.01 versus vehicle control group. Data shown in b–e are representative of two or more independent studies.
Extended Data Figure 8 Effects of co-administration of 6bK and dipeptidyl peptidase 4 (DPP4) inhibitor sitagliptin followed by oral glucose challenge.
a, DIO mice were first treated with either oral gavage of sitagliptin (4 mg kg−1, 5 ml kg−1 in sterile saline, n = 14) or saline alone (n = 12). After 30 min, each group of mice were treated either with a low dose of 6bK (40 mg kg−1, n = 7) or vehicle alone (n = 5), and after an additional 30 min all mice were given a bolus of glucose by gavage (3.0 g kg−1, 10 ml kg−1). Mice treated with the combination of sitagliptin and 6bK displayed glucose levels lower than baseline (t = 0) after 60 min. b, Blood glucose profile areas of sitagliptin and 6bk were similarly reduced by 60–64% compared to vehicle alone, and further 15% lower when sitagliptin and 6bK were co-administered. Symbol and bar colour-coding: black, vehicle alone; blue, sitagliptin alone; green, 6bK alone; purple, sitagliptin + 6bK. All data points and error bars represent mean ± s.e.m. Statistics were performed using a one-tail Student’s _t_-test. Significance levels shown in the figures are: *P < 0.017 (Bonferroni correction) versus vehicle control group, #P < 0.017 versus the 6bK cohort. See Supplementary Methods for a description of the AUC calculation. The data shown are from a study performed once.
Extended Data Figure 9 Glucose tolerance test AUC (area under the curve) calculations, and pyruvate tolerance test for gluconeogenesis during IDE inhibition.
a, AUC calculations for Fig. 2 data show that during OGTT, lean and DIO mice treated with 6bK display improved glucose tolerance, compared to vehicle controls and inactive bisepi-6bK. b, In contrast, during IPGTT both lean and DIO mice treated with 6bK display impaired glucose tolerance compared to vehicle or bisepi-6bK controls. The AUCs for 6bK versus vehicle treatments using _Ide_–/– mice are similar for both OGTT and IPGTT. c, Wild-type mice fasted overnight were injected i.p. with pyruvate (2.0 g kg−1) 30 min after treatment with 6bK, inactive analogue bisepi-6bK, or vehicle alone. d, Plasma hormone measurements 60 min post-PTT reveal elevated glucagon (top) but similar insulin (bottom) levels for the 6bK-treated cohort relative to bisepi-6bK or vehicle controls. e, RT–PCR analysis of liver samples 60-min post-PTT revealed elevated gluconeogenesis transcriptional markers for the 6bK-treated group relative to vehicle controls. All data points and error bars represent mean ± s.e.m. Statistics were performed using a two-tail Student’s _t_-test, and significance levels shown in the figures are *P < 0.05 versus vehicle control group; **P < 0.01 versus vehicle control group. See Supplementary Methods for a description of the AUC calculation. Data shown not involving knockout mice are representative of two or more independent studies; studies on knockout mice and PTT measurements were performed once.
Extended Data Figure 10 Model for the expanded roles of IDE in glucose homeostasis and gastric emptying based on the results of this study.
IDE inhibition increases the abundance and signalling of three key pancreatic peptidic hormones—insulin, amylin and glucagon—with the corresponding physiological effects shown in blue, blue and red, respectively.
Supplementary information
Supplementary Information
This file contains Supplementary results and Discussion, Supplementary Methods, Supplementary Tables 1-5, Supplementary Sequence and Supplementary References. (PDF 381 kb)
Animation of the X-ray co-crystal structure of IDE bound to macrocyclic inhibitor 6b.
The IDE domains 1, 2, 3, and 4 are colored green, blue, yellow, and red, respectively (2.7 Å resolution, pdb: 4LTE). Macrocycle 6b is represented as a ball-and-stick model, and the catalytic zinc atom is represented as an orange sphere. The macrocycle 6b is seen interacting within a 10 Å-deep hydrophobic pocket. The p-benzoyl-phenylalanine is shown in red, the cyclohexylalanine in blue, the fumarate linker in grey, and the D-lysine backbone in purple. The final scene shows a superimposition of 6b (shown as a surface rendering) on the co-crystal structure of insulin (shown in orange) within the IDE cavity (pdb: 2WBY), demonstrating that 6b binding to IDE is predicted to preclude substrate binding. (MPG 19772 kb)
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Maianti, J., McFedries, A., Foda, Z. et al. Anti-diabetic activity of insulin-degrading enzyme inhibitors mediated by multiple hormones.Nature 511, 94–98 (2014). https://doi.org/10.1038/nature13297
- Received: 09 August 2013
- Accepted: 27 March 2014
- Published: 21 May 2014
- Issue Date: 03 July 2014
- DOI: https://doi.org/10.1038/nature13297