BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2 (original) (raw)
References
Aguilera, A. & García-Muse, T. R loops: from transcription byproducts to threats to genome stability. Mol. Cell46, 115–124 (2012) ArticleCAS Google Scholar
Huertas, P. & Aguilera, A. Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination. Mol. Cell12, 711–721 (2003) ArticleCAS Google Scholar
Tous, C. & Aguilera, A. Impairment of transcription elongation by R-loops in vitro . Biochem. Biophys. Res. Commun.360, 428–432 (2007) ArticleCAS Google Scholar
Kaneko, S., Chu, C., Shatkin, A. & Manley, J. Human capping enzyme promotes formation of transcriptional R loops in vitro . Proc. Natl Acad. Sci. USA104, 17620–17625 (2007) ArticleADSCAS Google Scholar
Skourti-Stathaki, K., Proudfoot, N. & Gromak, N. Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination. Mol. Cell42, 794–805 (2011) ArticleCAS Google Scholar
Mischo, H. et al. Yeast Sen1 helicase protects the genome from transcription-associated instability. Mol. Cell41, 21–32 (2011) ArticleCAS Google Scholar
Tuduri, S. et al. Topoisomerase I suppresses genomic instability by preventing interference between replication and transcription. Nature Cell Biol.11, 1315–1324 (2009) ArticleCAS Google Scholar
Wellinger, R., Prado, F. & Aguilera, A. Replication fork progression is impaired by transcription in hyperrecombinant yeast cells lacking a functional THO complex. Mol. Cell. Biol.26, 3327–3334 (2006) ArticleCAS Google Scholar
Gan, W. et al. R-loop-mediated genomic instability is caused by impairment of replication fork progression. Genes Dev.25, 2041–2056 (2011) ArticleCAS Google Scholar
Helmrich, A., Ballarino, M. & Tora, L. Collisions between replication and transcription complexes cause common fragile site instability at the longest human genes. Mol. Cell44, 966–977 (2011) ArticleCAS Google Scholar
Domínguez-Sánchez, M., Barroso, S., Gómez-González, B., Luna, R. & Aguilera, A. Genome instability and transcription elongation impairment in human cells depleted of THO/TREX. PLoS Genet.7, e1002386 (2011) Article Google Scholar
Castellano-Pozo, M., García-Muse, T. & Aguilera, A. R-loops cause replication impairment and genome instability during meiosis. EMBO Rep.13, 923–929 (2012) ArticleCAS Google Scholar
Gómez-González, B. et al. Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles. EMBO J.30, 3106–3119 (2011) Article Google Scholar
Jani, D. et al. Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export. Nucleic Acids Res.40, 4562–4573 (2012) ArticleCAS Google Scholar
Cabal, G. et al. SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope. Nature441, 770–773 (2006) ArticleADSCAS Google Scholar
Umlauf, D. et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J. Cell Sci.126, 2656–2667 (2013) ArticleCAS Google Scholar
Gallardo, M., Luna, R., Erdjument-Bromage, H., Tempst, P. & Aguilera, A. Nab2p and the Thp1p–Sac3p complex functionally interact at the interface between transcription and mRNA metabolism. J. Biol. Chem.278, 24225–24232 (2003) ArticleCAS Google Scholar
Ellisdon, A., Dimitrova, L., Hurt, E. & Stewart, M. Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nature Struct. Mol. Biol.19, 328–336 (2012) ArticleCAS Google Scholar
Li, J. et al. DSS1 is required for the stability of BRCA2. Oncogene25, 1186–1194 (2006) ArticleCAS Google Scholar
Söderberg, O. et al. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nature Methods3, 995–1000 (2006) Article Google Scholar
El Hage, A., French, S., Beyer, A. & Tollervey, D. Loss of topoisomerase I leads to R-loop-mediated transcriptional blocks during ribosomal RNA synthesis. Genes Dev.24, 1546–1558 (2010) ArticleCAS Google Scholar
Ginno, P., Lott, P., Christensen, H., Korf, I. & Chédin, F. R-loop formation is a distinctive characteristic of unmethylated human CpG island promoters. Mol. Cell45, 814–825 (2012) ArticleCAS Google Scholar
Urtishak, K. et al. Timeless maintains genomic stability and suppresses sister chromatid exchange during unperturbed DNA replication. J. Biol. Chem.284, 8777–8785 (2009) ArticleCAS Google Scholar
Gravells, P. et al. Reduced FANCD2 influences spontaneous SCE and RAD51 foci formation in uveal melanoma and Fanconi anaemia. Oncogene32, 5338–5346 (2013) ArticleCAS Google Scholar
Bermejo, R. et al. The replication checkpoint protects fork stability by releasing transcribed genes from nuclear pores. Cell146, 233–246 (2011) ArticleCAS Google Scholar
Lomonosov, M., Anand, S., Sangrithi, M., Davies, R. & Venkitaraman, A. Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein. Genes Dev.17, 3017–3022 (2003) ArticleCAS Google Scholar
Schlacher, K. et al. Double-strand break repair-independent role for BRCA2 in blocking stalled replication fork degradation by MRE11. Cell145, 529–542 (2011) ArticleCAS Google Scholar
Schlacher, K., Wu, H. & Jasin, M. A distinct replication fork protection pathway connects Fanconi anemia tumor suppressors to RAD51-BRCA1/2. Cancer Cell22, 106–116 (2012) ArticleCAS Google Scholar
Moldovan, G.-L. & D’Andrea, A. How the Fanconi anemia pathway guards the genome. Annu. Rev. Genet.43, 223–249 (2009) ArticleCAS Google Scholar
Yang, H. et al. BRCA2 function in DNA binding and recombination from a BRCA2–DSS1-ssDNA structure. Science297, 1837–1848 (2002) ArticleADSCAS Google Scholar
ten Asbroek, A., van Groenigen, M., Nooij, M. & Baas, F. The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides. Eur. J. Biochem.269, 583–592 (2002) ArticleCAS Google Scholar
Bayani, J. & Squire, J. A. Sister chromatid exchange. Curr. Protoc. Cell Biol.25, 22.7.1–22.7.4 (2005) Article Google Scholar
Smith, A., Friedman, D., Yu, H., Carnahan, R. & Reynolds, A. ReCLIP (reversible cross-link immuno-precipitation): an efficient method for interrogation of labile protein complexes. PLoS ONE6, e16206 (2011) ArticleADSCAS Google Scholar