Evolutionary origin of the turtle skull (original) (raw)
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Acknowledgements
We thank J. Botha-Brink, E. Butler, S. Kaal, E. De Kock, J. Neveling and R. Smith for access to Eunotosaurus specimens. M. Fox and Z. Erasmus prepared fossil material. M. Colbert, J. Maisano, M. Hill and J. Thostenson are acknowledged for their help with the digital data. We thank A. Balanoff, D. Dykes, J. Gauthier, R. Hill, B. Rubidge, R. Smith and K. de Queiroz for helpful discussions. G.S.B. extends special thanks to the Academic Technologies Group at NYIT for their support in the digital visualization of anatomical data.
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Authors and Affiliations
- Department of Anatomy, New York Institute of Technology, College of Osteopathic Medicine, Old Westbury, 11568, New York, USA
G. S. Bever - Division of Paleontology, American Museum of Natural History, New York, 10024, New York, USA
G. S. Bever - Evolutionary Studies Institute, University of the Witwatersrand, Private Bag 3, P.O. WITS, Johannesburg, 2050, South Africa
G. S. Bever & Tyler R. Lyson - Department of Earth Sciences, Denver Museum of Nature and Science, Denver, 80205, Colorado, USA
Tyler R. Lyson - Department of Geology & Geophysics and Peabody Museum of Natural History, Yale University, New Haven, 06520, Connecticut, USA
Daniel J. Field & Bhart-Anjan S. Bhullar - Department of Organismal Biology and Anatomy, University of Chicago, Chicago, 60637, Illinois, USA
Bhart-Anjan S. Bhullar
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Contributions
G.S.B. designed the study, processed the CT data, performed the analytical work, constructed the figures, and wrote the paper. T.R.L. performed analytical work, assisted writing the paper, and assisted with figures. D.J.F. and B.-A.S.B. performed analytical work and assisted writing the paper.
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Correspondence toG. S. Bever.
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Extended data figures and tables
Extended Data Figure 1 Digital reconstruction of segmented cranial elements of Eunotosaurus africanus CM 777.
a, Palatal view with the lower jaws digitally removed and major roofing elements not rendered. b, Anteromedial view of left palate showing moderately sized suborbital fenestra. c, d, Posteromedial (c) and anterolateral (d) views of left quadrate, prootic, stapes, epipterygoid and midline parabasisphenoid. e, Right lateral view of anterior braincase wall and surrounding elements. Note sutural contact of prootic and quadrate. f–k, Lower jaws in dorsal (f), ventral (g), left lateral (h), medial (left jaw) (i), anterior (j), and posterior (k) views. an, angular; ar, articular; bs, parabasisphenoid; co, coronoid; d, dentary; ect, ectopterygoid; epi, epipterygoid; fr, frontal; ju, jugal; la, lacrimal; ls, laterosphenoid; mx, maxilla; op, opisthotic; pa, parietal; pf, prefrontal; pl, palatine; pm, premaxilla; po, postorbital; pof, postfrontal; pr, prootic; pra, prearticular; pt, pterygoid; q, quadrate; qj, quadratojugal; s, stapes; sq, squamosal; sof, suborbital fenestra; sp., splenial; st, supratemporal; sa, surangular; v, vomer; II, inferred exit point for orbital nerve; V, prootic incisure, exit point for trigeminal nerve.
Extended Data Figure 2 The juvenile skull of Eunotosaurus africanus (SAM-PK-K7909) showing clear expression of both LTF and UTF.
a, b, Left lateral view with the rostrum held horizontally (a) and slightly downturned (b). c, Close-up view of fenestrated cheek in right lateral view. The size of the fenestrae decreases in the late stages of postnatal ontogeny through expansion of the surrounding dermal bones. The upper temporal fenestra is eventually obscured by the late-stage ontogenetic development of an elongate supratemporal.
Extended Data Figure 3 Digitally segmented and reconstructed cranial elements of Eunotosaurus africanus (CM86-341).
a–d, Right lateral view (a), anterior (b), posterior (c), and right medial (d) views. an, angular; d, dentary; epi, epipterygoid; ju, jugal; la, lacrimal; ‘ls’, ‘laterosphenoid’; mx, maxilla; pa, parietal; pf, prefrontal; pl, palatine; po, postorbital; pof, postfrontal; pt, pterygoid; q, quadrate; qj, quadratojugal; sa + ar, surangular and articular; sq, squamosal; st, supratemporal; UTF, upper temporal fenestra; ?, unclear identity.
Extended Data Figure 4 Strict consensus of two most parsimonious recovered from total character matrix.
Bremer support values are provided for each clade (above line). Bootstrap values exceeding 50% are provided (below line). A _Eunotosaurus_–turtle clade is extremely well supported. That this pan-turtle lineage originated somewhere within the radiation of anatomically diapsid reptiles is well supported, although a refined phylogenetic position remains morphologically elusive. Tree length = 1,087; consistency index = 0.4013; retention index = 0.590.
Extended Data Figure 5 Bayesian tree topology derived from total matrix (50% majority rule consensus).
An exclusive _Eunotosaurus_–turtle clade is recovered with 100% posterior probability. This pan-turtle lineage is nested within the radiation of anatomically diapsid reptiles; however, in contrast to the parsimony solution, turtles are excluded from crown-group Diapsida. The Bayesian results agree with the parsimony in revealing strong support that: (1) Eunotosaurus is an early stem-group turtle; and (2) the ancestral stem turtle expressed a fully diapsid skull. The two analyses also agree that there is currently poor morphological support for a refined position of turtles within the greater diapsid radiation.
Extended Data Figure 6 Strict consensus of 13 most parsimonious trees recovered from cranial-only matrix.
The _Eunotosaurus_–turtle clade is recovered, which supports the hypothesis that the postcranial synapomorphies of Eunotosaurus and turtles are homologous and not the products of convergence. Tree length = 777; consistency index = 0.3956; retention index = 0.4743.
Extended Data Figure 7 Bayesian tree topology derived from cranial-only matrix (50% majority rule consensus).
When studied in isolation, cranial anatomy provides poor resolution of the deep divergences within Pan-Reptilia, but a _Eunotosaurus_–turtle signal is clearly present.
Supplementary information
Supplementary Information
This file contains a Supplementary Discussion, Supplementary References and Supplementary Tables 1-4. (PDF 1311 kb)
Supplementary Data
This file contains the data matrix used in phylogenetic analyses. (TXT 14 kb)
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Bever, G., Lyson, T., Field, D. et al. Evolutionary origin of the turtle skull.Nature 525, 239–242 (2015). https://doi.org/10.1038/nature14900
- Received: 28 April 2015
- Accepted: 03 July 2015
- Published: 02 September 2015
- Issue Date: 10 September 2015
- DOI: https://doi.org/10.1038/nature14900