Variation in the safety of induced pluripotent stem cell lines (original) (raw)

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Acknowledgements

We thank H. Abe, S. Yamaguchi, T. Tada, Y. Matsuzaki, T. Sunabori, H. Naka, M. Nishino, H. Higashi, T. Ichisaka and M. Imamura for technical assistance and scientific discussions. We also thank H. Niwa (CDB, RIKEN, JAPAN) for the EB3-ES cells, R. Farese (UCSF, USA) for the RF8 ES cells, A. Miyawaki (BSI, RIKEN, JAPAN) for the Venus gene and H. Miyoshi (BioResource Center, RIKEN, JAPAN) for the lentiviral vectors. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan; Japan Science and Technology Agency; National Institute of Biomedical Innovation; Japan Society for the Promotion of Science (JSPS), the Ministry of Health, Labor, and Welfare; the General Insurance Association of Japan and Keio Gijuku Academic Development Funds. K.M is supported by Research Fellowships for Young Scientists from JSPS.

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Authors and Affiliations

  1. Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
    Kyoko Miura, Takashi Aoi, Aki Okada, Kazutoshi Takahashi, Keisuke Okita, Masato Nakagawa, Michiyo Koyanagi, Koji Tanabe, Mari Ohnuki & Shinya Yamanaka
  2. Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Kyoko Miura, Masato Nakagawa, Koji Tanabe, Mari Ohnuki & Shinya Yamanaka
  3. Yamanaka iPS Cell Special Project, Japan Science and Technology Agency, Kawaguchi, Japan
    Kyoko Miura, Takashi Aoi, Aki Okada, Kazutoshi Takahashi, Keisuke Okita, Masato Nakagawa, Michiyo Koyanagi, Koji Tanabe, Mari Ohnuki & Shinya Yamanaka
  4. Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    Kyoko Miura, Yohei Okada, Daisuke Ogawa & Hideyuki Okano
  5. Kanrinmaru-Project, School of Medicine, Keio University, Tokyo, Japan
    Yohei Okada
  6. Department of Pathology, School of Medicine, Keio University, Tokyo, Japan
    Eiji Ikeda
  7. Gladstone Institute of Cardiovascular Disease, California, San Francisco, USA
    Shinya Yamanaka

Authors

  1. Kyoko Miura
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  2. Yohei Okada
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  3. Takashi Aoi
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  4. Aki Okada
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  5. Kazutoshi Takahashi
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  6. Keisuke Okita
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  7. Masato Nakagawa
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  8. Michiyo Koyanagi
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  9. Koji Tanabe
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  10. Mari Ohnuki
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  11. Daisuke Ogawa
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  12. Eiji Ikeda
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  13. Hideyuki Okano
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  14. Shinya Yamanaka
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Contributions

K.M. conducted most of the experiments. Y.O., T.A., A.O. and M.N. supported experiments and analyses. K. Takahashi, T.A., K.O., M.K., K. Tanabe and M.O. prepared undifferentiated iPS cells. E.I. and K. Takahashi assisted with the pathological analyses. D.O. assisted in some of the stereotactic transplantations. S.Y. and H.O. supervised the entire project. K.M. and S.Y. wrote the manuscript.

Corresponding authors

Correspondence toHideyuki Okano or Shinya Yamanaka.

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Miura, K., Okada, Y., Aoi, T. et al. Variation in the safety of induced pluripotent stem cell lines.Nat Biotechnol 27, 743–745 (2009). https://doi.org/10.1038/nbt.1554

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