A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs (original) (raw)

References

  1. Will, C. L. & Luhrmann, R. Spliceosomal UsnRNP biogenesis, structure and function. Curr. Opin. Cell Biol. 13, 290–301 (2001).
    Article CAS Google Scholar
  2. Achsel, T. et al. A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. EMBO J. 18, 5789–5802 (1999).
    Article CAS Google Scholar
  3. Seraphin, B. Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs. EMBO J. 14, 2089–2098 (1995).
    Article CAS Google Scholar
  4. Zhang, D., Abovich, N. & Rosbash, M. A biochemical function for the Sm complex. Mol. Cell 7, 319–329 (2001).
    Article CAS Google Scholar
  5. Mattaj, I. W. & De Robertis, E. M. Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins. Cell 40, 111–118 (1985).
    Article CAS Google Scholar
  6. Mattaj, I. W. in Structure and Function of Major and Minor Small Nuclear Ribonculeoprotein Particles (ed. Birnstiel, M.) 100–114 (Springer-Verlag, Berlin/NewYork, 1988).
    Book Google Scholar
  7. Neuman de Vegvar, H. E. & Dahlberg, J. E. Nucleocytoplasmic transport and processing of small nuclear RNA precursors. Mol. Cell. Biol. 10, 3365–3375 (1990).
    Article CAS Google Scholar
  8. Fischer, U. & Luhrmann, R. An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus. Science 249, 786–790 (1990).
    Article CAS Google Scholar
  9. Raker, V. A., Plessel, G. & Luhrmann, R. The snRNP core assembly pathway: identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro. EMBO J. 15, 2256–2269 (1996).
    Article CAS Google Scholar
  10. Raker, V. A., Hartmuth, K., Kastner, B. & Lührmann, R. Spliceosomal U snRNP core assembly: Sm proteins assemble onto a Sm site RNA nonanucleotide in a specific and thermodynamically stable manner. Mol. Cell. Biol. 19, 6554–6565 (1999).
    Article CAS Google Scholar
  11. Fischer, U., Liu, Q. & Dreyfuss, G. The SMN–SIP1 complex has an essential role in spliceosomal snRNP biogenesis. Cell 90, 1023–1029 (1997).
    Article CAS Google Scholar
  12. Buhler, D., Raker, V., Luhrmann, R. & Fischer, U. Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy. Hum. Mol. Genet. 8, 2351–2357 (1999).
    Article CAS Google Scholar
  13. Liu, Q., Fischer, U., Wang, F. & Dreyfuss, G. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell 90, 1013–1021 (1997).
    Article CAS Google Scholar
  14. Charroux, B. et al. Gemin3: a novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. J. Cell Biol. 147, 1181–1194 (1999).
    Article CAS Google Scholar
  15. Grundhoff, A. T. et al. Characterisation of DP103, a novel DEAD box protein that binds to the Epstein–Barr virus nuclear proteins EBNA2 and EBNA3C. J. Biol. Chem. 27, 19136–19144 (1999).
    Article Google Scholar
  16. Charroux, B. et al. Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. J. Cell Biol. 148, 1177–1186 (2000).
    Article CAS Google Scholar
  17. Meister, G. et al. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum. Mol. Genet. 9, 1977–1986 (2000).
    Article CAS Google Scholar
  18. Müller, B., Link, J. & Smythe, C. Assembly of U7 small nuclear ribonucleoprotein particle and histone RNA 3′ processing in Xenopus egg extracts. J. Biol. Chem. 275, 24284–24293 (2000).
    Article Google Scholar
  19. Jarmolowski, A. & Mattaj, I. W. The determinants for Sm protein binding to Xenopus U1 and U5 snRNAs are complex and non-identical. EMBO J. 12, 223–232 (1993).
    Article CAS Google Scholar
  20. Hunt, S. L., Hsuan, J. J., Totty, N. & Jackson, R. J. unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. Genes Dev. 13, 347–448 (1998).
    Google Scholar
  21. Johnson, J. L. & Craig, E. A. Protein folding in vivo: unraveling complex pathways. Cell 90, 201–204 (1997).
    Article CAS Google Scholar
  22. Pu, W. T., Krapivinsky, G. P., Krapivinsky, L. & Clapham, D. E. pICln inhibits snRNP biogenesis by binding core spliceosomal proteins. Mol. Cell. Biol. 19, 4113–4120 (1999).
    Article CAS Google Scholar
  23. Friesen, W. J., Massenet, S., Paushkin, S., Wyce, A. & Dreyfuss, G. SMN, the product of the spinal muscular atrophy gene, binds preferentially to dimethylarginine-containing protein targets. Mol. Cell 7, 1111–1117 (2001).
    Article CAS Google Scholar
  24. Friesen, W. J. & Dreyfuss, G. Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN). J. Biol. Chem. 275, 26370–26375 (2000).
    Article CAS Google Scholar
  25. Liu, Q. & Dreyfuss, G. A novel nuclear structure containing the survival of motor neurons protein. EMBO J. 15, 3555–3565 (1996).
    Article CAS Google Scholar
  26. Jones, K. W. et al. Direct interaction of the spinal muscular atrophy disease protein SMN with the core snoRNP protein fibrillarin. J. Biol. Chem. (in the press).
  27. Pellizzoni, L., Baccon, J., Charroux, B. & Dreyfuss, G. The survival of motor neurons (SMN) protein interacts with the snoRNP proteins fibrillarin and GAR1. Curr. Biol. 11, 1079–1088 (2001).
    Article CAS Google Scholar
  28. Murray, A. W. Cell cycle extracts. Methods Cell Biol. 36, 581–604 (1991).
    Article CAS Google Scholar
  29. Lerner, E. A., Lerner, M. R., Hardin, J. A., Janeway, C. A. & Steitz, J. A. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proc. Natl. Acad. Sci. USA 78, 2737–2741 (1981).
    Article CAS Google Scholar

Download references