Extra centrosomes and/or chromosomes prolong mitosis in human cells (original) (raw)

Nature Cell Biology volume 10, pages 748–751 (2008)Cite this article

Abstract

Using laser microsurgery and cell fusion we have explored how additional centrosomes and/or chromosomes influence the duration of mitosis in human cells. We found that doubling the chromosome number added approximately 10 min to a 20 min division, whereas doubling the number of centrosomes added approximately 30 min more. Extra centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the spindle assembly checkpoint. Thus mitosis can be prolonged by non-genetic means and extra chromosomes and centrosomes probably contribute to the elevated mitotic index seen in many tumours.

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Acknowledgements

This work was supported by National Institutes of General Medical Sciences grants 40198 (to C.L.R.) and 59363 (to A.K.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of General Medical Sciences or the National Institutes of Health.

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Authors and Affiliations

  1. Division of Molecular Medicine, N.Y.S. Department of Health, Wadsworth Center, Empire State Plaza, Albany, 12201-0509, New York, USA
    Zhenye Yang, Jadranka Lončarek, Alexey Khodjakov & Conly L. Rieder
  2. Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, 12222, New York, USA
    Alexey Khodjakov & Conly L. Rieder
  3. Marine Biology Laboratory, 7 MBL Street, Woods Hole, 02543, MA, USA
    Alexey Khodjakov & Conly L. Rieder

Authors

  1. Zhenye Yang
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  2. Jadranka Lončarek
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  3. Alexey Khodjakov
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  4. Conly L. Rieder
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Contributions

Z.Y. conducted the experiments and helped with the data analysis; J.L. assisted with the laser ablation work; A.K. and C.L.R. planned and supervised the project and data analyses.

Corresponding author

Correspondence toConly L. Rieder.

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The authors declare no competing financial interests.

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Yang, Z., Lončarek, J., Khodjakov, A. et al. Extra centrosomes and/or chromosomes prolong mitosis in human cells.Nat Cell Biol 10, 748–751 (2008). https://doi.org/10.1038/ncb1738

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