Phosphorothioation of DNA in bacteria by dnd genes (original) (raw)

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Acknowledgements

We are very grateful to D. Hopwood for his continuous support and encouragement throughout our studies on the DNA S-modification system. We are grateful to L. Cui, B. Chen and J. Son (MIT) and J. Marr (Agilent) for expert assistance with mass spectrometry, to Agilent for access to the XCT Ultra ion-trap mass spectrometer, to T. Murase (Tottori University, Japan) for the generous gift of S. enterica serovar Cerro 87, and to J. Fleckenstein (University of Tennessee Health Sciences Center) for a generous gift of the E. coli strain B7A. Financial support for this work was provided by the Ministry of Science and Technology, the National Science Foundation of China, the Ministry of Education of China, the Shanghai Municipal Council of Science and Technology, the US National Cancer Institute and a Center Grant from the US National Institute of Environmental Health Sciences.

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Author notes

  1. Lianrong Wang and Shi Chen: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai, 200030, China
    Lianrong Wang, Shi Chen, Tiegang Xu, Xiufen Zhou, Delin You & Zixin Deng
  2. Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, Massachusetts, USA
    Lianrong Wang, John S Wishnok & Peter C Dedon
  3. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, Massachusetts, USA
    Shi Chen
  4. MIT Center for Environmental Health Sciences, 77 Massachusetts Avenue, Cambridge, 02139, Massachusetts, USA
    Koli Taghizadeh, John S Wishnok & Peter C Dedon

Authors

  1. Lianrong Wang
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  2. Shi Chen
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  3. Tiegang Xu
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  4. Koli Taghizadeh
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  5. John S Wishnok
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  6. Xiufen Zhou
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  7. Delin You
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  8. Zixin Deng
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  9. Peter C Dedon
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Contributions

L.W. and S.C. contributed equally to this work and were responsible for all of the studies related to the chemical and biochemical characterization of the phosphorothioate bonds. T.X. performed the Dnd phenotype studies depicted in Supplementary Figure 1. T.X., X.Z., D.Y. and Z.D. participated in the development of biochemical methods and the bacterial strains used in the studies. K.T. and J.S.W. developed analytical methods and assisted with performing chromatographic and mass spectrometric studies. P.C.D. was responsible for planning and oversight with the chemical and biochemical characterization of the phosphorothioate bonds. L.W., S.C., Z.D. and P.C.D. wrote the paper. All authors discussed the results and assisted with editing of the manuscript.

Corresponding authors

Correspondence toZixin Deng or Peter C Dedon.

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Wang, L., Chen, S., Xu, T. et al. Phosphorothioation of DNA in bacteria by dnd genes.Nat Chem Biol 3, 709–710 (2007). https://doi.org/10.1038/nchembio.2007.39

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