Identification of a specific inhibitor of the histone methyltransferase SU(VAR)3-9 (original) (raw)
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- Published: 17 July 2005
Nature Chemical Biology volume 1, pages 143–145 (2005)Cite this article
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Abstract
Histone methylation plays a key role in establishing and maintaining stable gene expression patterns during cellular differentiation and embryonic development. Here, we report the characterization of the fungal metabolite chaetocin as the first inhibitor of a lysine-specific histone methyltransferase. Chaetocin is specific for the methyltransferase SU(VAR)3-9 both in vitro and in vivo and may therefore be used to study heterochromatin-mediated gene repression.
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Acknowledgements
Work in A.I.'s laboratory is funded by grants from the Deutsche Forschungsgemeinschaft (IM23/4-1). D.G. is supported by a predoctoral grant from the University of Munich (FöFoLe #12). T.B. is funded by a long-term postdoctoral fellowship from the European Molecular Biology Organisation (ALTF 642-2003). We thank T. Jenuwein, E. Selker, Y. Shinkai, Y. Cho and P. Singh for valuable reagents, B. Czermin and L. Israel for help with the protein expression and MS analysis, and J. Sutcliffe for critical reading of the manuscript.
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- Ernst Roemer
Present address: Institut für Pflanzenbiochemie, Universität Halle, Weinberg 3, Halle (Saale), 06120, Germany
Authors and Affiliations
- Department of Molecular Biology, Adolf Butenandt Institute, Histone Modifications Group, Ludwig-Maximillians University of Munich, Schillerstr. 44, Munich, 80336, Germany
Dorothea Greiner, Tiziana Bonaldi, Ragnhild Eskeland & Axel Imhof - Abteilung Molekulare Naturstoff-Forschung, Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie–Hans-Knöll-Institut für Naturstoff-Forschung, Beutenbergstrasse 11a, Jena, 07745, Germany
Ernst Roemer
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- Dorothea Greiner
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Correspondence toAxel Imhof.
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Greiner, D., Bonaldi, T., Eskeland, R. et al. Identification of a specific inhibitor of the histone methyltransferase SU(VAR)3-9.Nat Chem Biol 1, 143–145 (2005). https://doi.org/10.1038/nchembio721
- Received: 10 March 2005
- Accepted: 22 June 2005
- Published: 17 July 2005
- Issue Date: August 2005
- DOI: https://doi.org/10.1038/nchembio721