A genome-wide association study identifies variants in the HLA-DP locus associated with chronic hepatitis B in Asians (original) (raw)
- Letter
- Published: 06 April 2009
- Sukanya Wattanapokayakit3,
- Hidenori Ochi4,5,
- Takahisa Kawaguchi4,
- Atsushi Takahashi4,
- Naoya Hosono4,
- Michiaki Kubo4,
- Tatsuhiko Tsunoda4,
- Naoyuki Kamatani4,
- Hiromitsu Kumada6,
- Aekkachai Puseenam7,
- Thanyachai Sura7,
- Yataro Daigo1,2,
- Kazuaki Chayama4,5,
- Wasun Chantratita8,
- Yusuke Nakamura1,4 &
- …
- Koichi Matsuda1
Nature Genetics volume 41, pages 591–595 (2009)Cite this article
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Abstract
Chronic hepatitis B is a serious infectious liver disease that often progresses to liver cirrhosis and hepatocellular carcinoma; however, clinical outcomes after viral exposure vary enormously among individuals1. Through a two-stage genome-wide association study using 786 Japanese chronic hepatitis B cases and 2,201 controls, we identified a significant association of chronic hepatitis B with 11 SNPs in a region including HLA-DPA1 and HLA-DPB1. We validated these associations by genotyping two SNPs from the region in three additional Japanese and Thai cohorts consisting of 1,300 cases and 2,100 controls (combined P = 6.34 × 10−39 and 2.31 × 10−38, OR = 0.57 and 0.56, respectively). Subsequent analyses revealed risk haplotypes (HLA-DPA1*0202-DPB1*0501 and HLA-DPA1*0202-DPB1*0301, OR = 1.45 and 2.31, respectively) and protective haplotypes (HLA-DPA1*0103-DPB1*0402 and HLA-DPA1*0103-DPB1*0401, OR = 0.52 and 0.57, respectively). Our findings show that genetic variants in the HLA-DP locus are strongly associated with risk of persistent infection with hepatitis B virus.
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Acknowledgements
We thank K. Tokunaga for useful advice of on HLA-DP genotyping and interpretation, and technical staff of Laboratory for Genotyping Development at RIKEN for SNP genotyping at the first and second stages of the GWAS. We are also grateful to members of Hiroshima Liver Study Group and The Rotary Club of Osaka-Midosuji District 2660 Rotary International in Japan for supporting our study. This work was conducted as a part of the BioBank Japan Project that was supported by the Ministry of Education, Culture, Sports, Sciences and Technology of the Japanese government.
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Authors and Affiliations
- Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, the University of Tokyo, Tokyo, Japan
Yoichiro Kamatani, Yataro Daigo, Yusuke Nakamura & Koichi Matsuda - Department of Medical Genome Sciences, Graduate School of Frontier Sciences, the University of Tokyo, Tokyo, Japan
Yoichiro Kamatani & Yataro Daigo - Department of Medical Sciences, Medical Genetic Section, National Institute of Health, Ministry of Public Health, Nonthaburi, Thailand
Sukanya Wattanapokayakit - Center for Genomic Medicine, RIKEN, Kanagawa, Japan
Hidenori Ochi, Takahisa Kawaguchi, Atsushi Takahashi, Naoya Hosono, Michiaki Kubo, Tatsuhiko Tsunoda, Naoyuki Kamatani, Kazuaki Chayama & Yusuke Nakamura - Department of Medicine and Molecular Science, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
Hidenori Ochi & Kazuaki Chayama - Department of Hepatology, Toranomon Hospital, Tokyo, Japan
Hiromitsu Kumada - Department of Medicine, Faculty of Medicine, Ramathidi Hospital, Mahidol University, Bangkok, Thailand
Aekkachai Puseenam & Thanyachai Sura - Department of Pathology, Virology and Molecular Microbiology Unit, Faculty of Medicine, Ramathidi Hospital, Mahidol University, Bangkok, Thailand
Wasun Chantratita
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Contributions
Y.N. conceived the study; Y.N., Y.K., Y.D., M.K. and K.M. designed the study; Y.K., S.W., H.O. and N.H. performed genotyping; Y.K., T.T., M.K., N.K., Y.N. and K.M. wrote the manuscript; T.K., A.T., T.T. and N.K. performed data analysis at the genome-wide phase; Y.N., K.M. and M.K. managed DNA samples belong to BioBankJapan; K.C. and H.K. managed second replication samples; W.C., A.P. and T.S. managed third replication samples in Thailand; Y.K. summarized the whole results; Y.N. obtained funding for the study.
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Correspondence toYusuke Nakamura.
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Kamatani, Y., Wattanapokayakit, S., Ochi, H. et al. A genome-wide association study identifies variants in the HLA-DP locus associated with chronic hepatitis B in Asians.Nat Genet 41, 591–595 (2009). https://doi.org/10.1038/ng.348
- Received: 10 November 2008
- Accepted: 16 January 2009
- Published: 06 April 2009
- Issue Date: May 2009
- DOI: https://doi.org/10.1038/ng.348