SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas (original) (raw)

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Acknowledgements

We thank J. Francis, J. Cho, A. Schinzel, R. Firestein, I. Guney and J. Boehm for technical advice and discussions. A.J.B. is supported by the Harvard Clinical Investigator Training Program and American Society of Clinical Oncology. H.W. is supported by a Ruth L. Kirschstein NRSA T32 institutional training grant. R.G.V. is supported by the KWF Kankerbestrijding. The Morphology, Molecular Biology and Cell Culture Core of 5P01CA098101-05 (A.K.R.) provided tissue processing for this project. This work was supported by US Department of Defense VITAL grant (J.D.M, A.F.G.) and by National Cancer Institute grants K08CA134931 (A.J.B.), P50CA70907 (J.D.M, A.F.G.), R33CA128625 (W.C.H.), R01CA071606-12 (D.G.B.), P01CA098101-05 (A.K.R.) and R01CA109038 and P50CA90578 (M.M.). Funding for lung SCC SNP arrays was provided by Genentech, Inc. Funding was provided by the Sara Thomas Monopoli Lung Cancer Research Fund and the Seaman Corporation Fund for Lung Cancer Research.

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Author notes

1. Adam J Bass and Hideo Watanabe: These authors contributed equally to this work.

Authors and Affiliations

1. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA
   Adam J Bass, Hideo Watanabe, Craig H Mermel, Soyoung Yu, Roel G Verhaak, So Young Kim, Leslie Wardwell, Alex H Ramos, Michele S Woo, Barbara A Weir, Rameen Beroukhim, Amit Dutt, Orit Rozenblatt-Rosen, Piotr Dziunycz, Justin Komisarof, Ramesh A Shivdasani, Kwok K Wong, William C Hahn & Matthew Meyerson
2. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
   Ramesh A Shivdasani & Kwok K Wong
3. Center for Cancer Genome Discovery, Dana-Farber Cancer Institute, Boston, Massachusetts, USA
   Hideo Watanabe, Craig H Mermel, Soyoung Yu, Roel G Verhaak, So Young Kim, Leslie Wardwell, Alex H Ramos, Michele S Woo, Barbara A Weir, Amit Dutt, Orit Rozenblatt-Rosen & Matthew Meyerson
4. Broad Institute, Cambridge, Massachusetts, USA
   Adam J Bass, Hideo Watanabe, Craig H Mermel, Roel G Verhaak, Pablo Tamayo, Irit Gat-Viks, Alex H Ramos, Michele S Woo, Barbara A Weir, Gad Getz, Rameen Beroukhim, Michael O'Kelly, Amit Dutt, Aviv Regev, William C Hahn & Matthew Meyerson
5. Department of Pathology, Weill Medical College of Cornell University, New York, New York, USA
   Sven Perner, Christopher J LaFargue, Patrick Wagner & Mark A Rubin
6. Institute of Pathology, Comprehensive Cancer Center, University Hospitals of Tuebingen, Tuebingen, Germany
   Sven Perner, Veit Scheble & Theresia Wilbertz
7. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
   Lucian R Chirieac & Matthew Meyerson
8. Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
   Changqing Ma, Shilpa Rao, Hiroshi Nakagawa, Douglas B Stairs, Marcia S Brose & Anil K Rustgi
9. Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania, USA
   Hiroshi Nakagawa, Douglas B Stairs & Anil K Rustgi
10. Department of Surgery, Section of Thoracic Surgery, Ann Arbor, Michigan, USA
    Lin Lin & David G Beer
11. Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA
    Thomas J Giordano
12. University of Texas Southwestern Medical Center, Dallas, Texas, USA
    John D Minna & Adi F Gazdar
13. University Health Network and Princess Margaret Hospital, Toronto, Canada
    Chang Qi Zhu & Ming-Sound Tsao
14. Department of Otorhinolaryngology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Marcia S Brose
15. School of Medicine, University of São Paolo, São Paolo, Brazil
    Ivan Cecconello, Ulysses Ribeiro Jr & Suely K Marie
16. Section of Oncology, University of Bergen, Bergen, Norway
    Olav Dahl
17. Department of Genetics, University of Pennsylvania, Philadelphia, Pennsylvania, USA.,
    Anil K Rustgi

Authors

1. Adam J Bass
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2. Hideo Watanabe
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3. Craig H Mermel
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4. Soyoung Yu
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5. Sven Perner
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6. Roel G Verhaak
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7. So Young Kim
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8. Leslie Wardwell
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9. Pablo Tamayo
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10. Irit Gat-Viks
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11. Alex H Ramos
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12. Michele S Woo
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13. Barbara A Weir
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14. Gad Getz
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15. Rameen Beroukhim
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16. Michael O'Kelly
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17. Amit Dutt
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18. Orit Rozenblatt-Rosen
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19. Piotr Dziunycz
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20. Justin Komisarof
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21. Lucian R Chirieac
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22. Christopher J LaFargue
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23. Veit Scheble
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24. Theresia Wilbertz
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25. Changqing Ma
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26. Shilpa Rao
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27. Hiroshi Nakagawa
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28. Douglas B Stairs
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29. Lin Lin
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30. Thomas J Giordano
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31. Patrick Wagner
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32. John D Minna
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33. Adi F Gazdar
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34. Chang Qi Zhu
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35. Marcia S Brose
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36. Ivan Cecconello
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37. Ulysses Ribeiro Jr
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38. Suely K Marie
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39. Olav Dahl
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40. Ramesh A Shivdasani
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41. Ming-Sound Tsao
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42. Mark A Rubin
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43. Kwok K Wong
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44. Aviv Regev
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45. William C Hahn
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46. David G Beer
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47. Anil K Rustgi
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48. Matthew Meyerson
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Contributions

L.R.C., H.N., D.B.S., L.L., T.J.G., J.D.M., A.F.G., M.S.B., I.C., U.R. Jr., S.K.M., O.D., M.-S.T., K.K.W. and D.G.B. provided samples for analysis. S.P., P.W., C.J.L., V.S., T.W. and M.A.R. performed and analyzed FISH assays and performed immunohistochemistry analysis. C.H.M., R.G.V., P.T., I.G.-V., A.H.R., B.A.W., G.G., R.B., M.O., J.K., C.M., S.R. and A.R. performed bioinformatic analysis. A.J.B., H.W., S.Y., S.Y.K., L.W., P.D. and C.Q.Z. performed laboratory experiments. O.R.-R., A.D. and M.S.W. provided expression constructs and extensive technical expertise. R.A.S. and W.C.H. provided guidance regarding experimental design and data interpretation. A.J.B., H.W., A.K.R. and M.M. designed the study, analyzed data and prepared the manuscript. All authors discussed results and edited the manuscript.

Corresponding author

Correspondence toMatthew Meyerson.

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Competing interests

The authors Matthew Meyerson, William C Hahn and Ramesh A Shivdasani serve as consultants to Novartis.

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Bass, A., Watanabe, H., Mermel, C. et al. SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas.Nat Genet 41, 1238–1242 (2009). https://doi.org/10.1038/ng.465

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• Received: 18 February 2009
• Accepted: 11 September 2009
• Published: 04 October 2009
• Issue Date: November 2009
• DOI: https://doi.org/10.1038/ng.465