Chipping away at the chip bias: RNA degradation in microarray analysis (original) (raw)
- Correspondence
- Published: 01 December 2003
- Sandya Lyianarachchi1,
- David Newsom1,
- Marko I Klisovic2,
- uido Marcucci2 &
- …
- Karl Kornacker3
Nature Genetics volume 35, pages 292–293 (2003)Cite this article
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An Erratum to this article was published on 01 January 2004
To the editor:
If one RNA sample was intact and the other was degraded during isolation, up to three-quarters of the differential gene expression measured was due solely to differences in RNA integrity between two samples (Fig. 1d). Supplementary Figure 1 online shows changes in mRNA levels caused by alteration of RNA integrity. This effect was independent of the algorithm applied to raw data analysis (Supplementary Tables 2, 3 and 4 online).
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Figure 1: Quantification of RNA integrity and consequences of degradation for expression analysis.
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Acknowledgements
We thank H. Klump, S. M. Tanner and K. Becknell for their critical reading of the manuscript. This work is supported in part by a grant from the US National Cancer Institute.
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Authors and Affiliations
- Division of Human Cancer Genetics, The Ohio State University, Columbus, 43210, Ohio, USA
Herbert Auer, Sandya Lyianarachchi & David Newsom - Department of Internal Medicine, The Ohio State University, Columbus, 43210, Ohio, USA
Marko I Klisovic & uido Marcucci - Division of Sensory Biophysics, The Ohio State University, Columbus, 43210, Ohio, USA
Karl Kornacker
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Correspondence toHerbert Auer.
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Auer, H., Lyianarachchi, S., Newsom, D. et al. Chipping away at the chip bias: RNA degradation in microarray analysis.Nat Genet 35, 292–293 (2003). https://doi.org/10.1038/ng1203-292
- Issue Date: 01 December 2003
- DOI: https://doi.org/10.1038/ng1203-292