The microRNA-producing enzyme Dicer1 is essential for zebrafish development (original) (raw)

Nature Genetics volume 35, pages 217–218 (2003)Cite this article

Abstract

MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

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Acknowledgements

We thank M. Koster, J. Mudde, M. den Broeder and A. Keijser for help with finding of the Dicer mutants; the Sanger Centre for sequencing the BAC containing the dicer1 gene; and D. Jongejan-Zivkovic, M. Tijsterman and R. Ketting for discussions. This work is supported by a grant from the Netherlands Organization for Scientific research (NWO).

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Authors and Affiliations

  1. Department of Functional Genomics, Hubrecht Laboratory, Utrecht, 3584 CT, The Netherlands
    Erno Wienholds, Marco J Koudijs, Freek J M van Eeden, Edwin Cuppen & Ronald H A Plasterk

Authors

  1. Erno Wienholds
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  2. Marco J Koudijs
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  3. Freek J M van Eeden
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  4. Edwin Cuppen
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  5. Ronald H A Plasterk
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Corresponding author

Correspondence toRonald H A Plasterk.

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The authors declare no competing financial interests.

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Wienholds, E., Koudijs, M., van Eeden, F. et al. The microRNA-producing enzyme Dicer1 is essential for zebrafish development.Nat Genet 35, 217–218 (2003). https://doi.org/10.1038/ng1251

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