Dicer is essential for mouse development (original) (raw)
- Brief Communication
- Published: 05 October 2003
- Sang Yong Kim1,
- Michelle A Carmell1,2,
- Elizabeth P Murchison1,
- Heather Alcorn3,
- Mamie Z Li4,
- Alea A Mills1,
- Stephen J Elledge4,
- Kathryn V Anderson3 &
- …
- Gregory J Hannon1
Nature Genetics volume 35, pages 215–217 (2003)Cite this article
- 8167 Accesses
- 1507 Citations
- 20 Altmetric
- Metrics details
A Corrigendum to this article was published on 01 November 2003
Abstract
To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with _Dicer1_-null embryos depleted of stem cells. Coupled with our inability to generate viable _Dicer1_-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Hannon, G.J. Nature 418, 244–251 (2002).
Article CAS Google Scholar - Bernstein, E., Caudy, A.A., Hammond, S.M. & Hannon, G.J. Nature 409, 363–366 (2001).
Article CAS Google Scholar - Ketting, R.F. et al. Genes Dev. 15, 2654–2659 (2001).
Article CAS Google Scholar - Knight, S.W. & Bass, B.L. Science 293, 2269–2271 (2001).
Article CAS Google Scholar - Hutvagner, G. et al. Science 293, 834–838 (2001).
Article CAS Google Scholar - Grishok, A. et al. Cell 106, 23–34 (2001).
Article CAS Google Scholar - Hutvagner, G. & Zamore, P.D. Science 1, 1 (2002).
Google Scholar - Zhang, P., Li, M.Z. & Elledge, S.J. Nat. Genet. 30, 31–39 (2002).
Article Google Scholar - Blaszczyk, J. et al. Structure (Camb) 9, 1225–1236 (2001).
Article CAS Google Scholar - Carmell, M.A., Xuan, Z., Zhang, M.Q. & Hannon, G.J. Genes Dev. 16, 2733–2742 (2002).
Article CAS Google Scholar - Nichols, J. et al. Cell 95, 379–391 (1998).
Article CAS Google Scholar - Niwa, H., Masui, S., Chambers, I., Smith, A.G. & Miyazaki, J. Mol. Cell Biol. 22, 1526–1536 (2002).
Article CAS Google Scholar - Avilion, A.A. et al. Genes Dev. 17, 126–140 (2003).
Article CAS Google Scholar - Yeom, Y.I. et al. Development 122, 881–894 (1996).
CAS PubMed Google Scholar - Shauer, S.E., Jacobsen, S.E., Meinke, D.W. & Ray, A. Trends Plant Sci. 7, 487–491 (2002).
Article Google Scholar
Acknowledgements
We thank L. Bianco and J. Coblentz for assistance with animals; B. Holdener, M.J. Garcia-Garcia and the Stony Brook University Histology Facility for help with initial embryo dissections; and M. Zhang and Z. Xuan for bioinformatics. E.M. is a Elisabeth Sloane Livingston Fellow of the Watson School of Biological Sciences. M.C. is supported by a US Army Breast Cancer Research Program Predoctoral Fellowship. This work was supported in part by grants from the US National Institutes of Health (G.J.H, S.J.E. and K.V.A) and the Howard Hughes Medical Institute (S.J.E.).
Author information
Authors and Affiliations
- Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, 11724, New York, USA
Emily Bernstein, Sang Yong Kim, Michelle A Carmell, Elizabeth P Murchison, Alea A Mills & Gregory J Hannon - Program in Genetics, State University of New York at Stony Brook, Stony Brook, 11794, New York, USA
Emily Bernstein & Michelle A Carmell - Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New York, 10021, New York, USA
Heather Alcorn & Kathryn V Anderson - Department of Biochemistry and Molecular Biology and Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, Houston, 77030, Texas, USA
Mamie Z Li & Stephen J Elledge
Authors
- Emily Bernstein
You can also search for this author inPubMed Google Scholar - Sang Yong Kim
You can also search for this author inPubMed Google Scholar - Michelle A Carmell
You can also search for this author inPubMed Google Scholar - Elizabeth P Murchison
You can also search for this author inPubMed Google Scholar - Heather Alcorn
You can also search for this author inPubMed Google Scholar - Mamie Z Li
You can also search for this author inPubMed Google Scholar - Alea A Mills
You can also search for this author inPubMed Google Scholar - Stephen J Elledge
You can also search for this author inPubMed Google Scholar - Kathryn V Anderson
You can also search for this author inPubMed Google Scholar - Gregory J Hannon
You can also search for this author inPubMed Google Scholar
Corresponding author
Correspondence toGregory J Hannon.
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Bernstein, E., Kim, S., Carmell, M. et al. Dicer is essential for mouse development.Nat Genet 35, 215–217 (2003). https://doi.org/10.1038/ng1253
- Received: 17 August 2003
- Accepted: 19 September 2003
- Published: 05 October 2003
- Issue Date: 01 November 2003
- DOI: https://doi.org/10.1038/ng1253