Anchored multiplex PCR for targeted next-generation sequencing (original) (raw)
- Technical Report
- Published: 10 November 2014
- Matthew Liebers1,
- Boryana Zhelyazkova1,
- Yi Cao1,
- Divya Panditi1,
- Kerry D Lynch1,
- Juxiang Chen1,3,
- Hayley E Robinson1,
- Hyo Sup Shim1,4,
- Juliann Chmielecki5,
- William Pao5,
- Jeffrey A Engelman6,
- A John Iafrate1,6 &
- …
- Long Phi Le1,6
Nature Medicine volume 20, pages 1479–1484 (2014)Cite this article
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Abstract
We describe a rapid target enrichment method for next-generation sequencing, termed anchored multiplex PCR (AMP), that is compatible with low nucleic acid input from formalin-fixed paraffin-embedded (FFPE) specimens. AMP is effective in detecting gene rearrangements (without prior knowledge of the fusion partners), single nucleotide variants, insertions, deletions and copy number changes. Validation of a gene rearrangement panel using 319 FFPE samples showed 100% sensitivity (95% confidence limit: 96.5–100%) and 100% specificity (95% confidence limit: 99.3–100%) compared with reference assays. On the basis of our experience with performing AMP on 986 clinical FFPE samples, we show its potential as both a robust clinical assay and a powerful discovery tool, which we used to identify new therapeutically important gene fusions: _ARHGEF2_-NTRK1 and _CHTOP_-NTRK1 in glioblastoma, _MSN_-ROS1, TRIM4-BRAF, VAMP2-NRG1, TPM3-NTRK1 and RUFY2-RET in lung cancer, FGFR2-CREB5 in cholangiocarcinoma and PPL-NTRK1 in thyroid carcinoma. AMP is a scalable and efficient next-generation sequencing target enrichment method for research and clinical applications.
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Acknowledgements
This study was supported by a US National Institutes of Health grant (R21CA161590) to A.J.I. and a postdoctoral fellowship from the Swedish Research Council (350-2012-368) to Z.Z.
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Authors and Affiliations
- Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA
Zongli Zheng, Matthew Liebers, Boryana Zhelyazkova, Yi Cao, Divya Panditi, Kerry D Lynch, Juxiang Chen, Hayley E Robinson, Hyo Sup Shim, A John Iafrate & Long Phi Le - Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden
Zongli Zheng - Department of Neurosurgery, Shanghai Changzheng Hospital, Shanghai, China
Juxiang Chen - Department of Pathology, Yonsei University College of Medicine, Seoul, Korea
Hyo Sup Shim - Vanderbilt University Medical Center, Nashville, Tennessee, USA
Juliann Chmielecki & William Pao - Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA
Jeffrey A Engelman, A John Iafrate & Long Phi Le
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- Zongli Zheng
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Contributions
Z.Z., M.L., B.Z., Y.C., D.P., K.D.L., J.C., H.E.R., H.S.S., J.C., W.P. & J.A.E. conducted the experiments; Z.Z. and L.P.L. conducted the data analyses; Z.Z. and L.P.L. wrote the manuscript; A.J.I. supervised the project.
Corresponding authors
Correspondence toA John Iafrate or Long Phi Le.
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Competing interests
A provisional patent has been filed by A.J.I., L.P.L. and Z.Z. for the anchored multiplex PCR technology with the US Patent Office. A.J.I., L.P.L. and Z.Z. are equity holders in Enzymatics, Inc., a licensee of the technology.
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Zheng, Z., Liebers, M., Zhelyazkova, B. et al. Anchored multiplex PCR for targeted next-generation sequencing.Nat Med 20, 1479–1484 (2014). https://doi.org/10.1038/nm.3729
- Received: 31 July 2013
- Accepted: 29 July 2014
- Published: 10 November 2014
- Issue Date: December 2014
- DOI: https://doi.org/10.1038/nm.3729