Single-cell in situ RNA profiling by sequential hybridization (original) (raw)

Nature Methods volume 11, pages 360–361 (2014)Cite this article

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To the Editor:

In our previous paper, Lubeck and Cai1, we used super-resolution microscopy to resolve a large number of mRNAs in single cells. In this Correspondence, we present a sequential barcoding scheme to multiplex different mRNAs.

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Figure 1: Sequential barcoding.

References

  1. Lubeck, E. & Cai, L. Nat. Methods 9, 743–748 (2012).
    Article CAS Google Scholar
  2. Ke, R. et al. Nat. Methods 10, 857–860 (2013).
    Article CAS Google Scholar
  3. Levesque, M.J., Ginart, P., Wei, Y. & Raj, A. Nat. Methods 10, 865–867 (2013).
    Article CAS Google Scholar
  4. Levesque, M.J. & Raj, A. Nat. Methods 10, 246–248 (2013).
    Article CAS Google Scholar

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Acknowledgements

This work is funded by US National Institutes of Health single-cell analysis program award R01HD075605.

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Author notes

  1. Eric Lubeck and Ahmet F Coskun: These authors contributed equally to this work.

Authors and Affiliations

  1. Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA
    Eric Lubeck, Ahmet F Coskun, Timur Zhiyentayev, Mubhij Ahmad & Long Cai

Authors

  1. Eric Lubeck
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  2. Ahmet F Coskun
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  3. Timur Zhiyentayev
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  4. Mubhij Ahmad
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  5. Long Cai
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Corresponding author

Correspondence toLong Cai.

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The authors declare no competing financial interests.

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Lubeck, E., Coskun, A., Zhiyentayev, T. et al. Single-cell in situ RNA profiling by sequential hybridization.Nat Methods 11, 360–361 (2014). https://doi.org/10.1038/nmeth.2892

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