Single-cell in situ RNA profiling by sequential hybridization (original) (raw)
- Correspondence
- Published: 28 March 2014
Nature Methods volume 11, pages 360–361 (2014)Cite this article
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To the Editor:
In our previous paper, Lubeck and Cai1, we used super-resolution microscopy to resolve a large number of mRNAs in single cells. In this Correspondence, we present a sequential barcoding scheme to multiplex different mRNAs.
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Figure 1: Sequential barcoding.
References
- Lubeck, E. & Cai, L. Nat. Methods 9, 743–748 (2012).
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Acknowledgements
This work is funded by US National Institutes of Health single-cell analysis program award R01HD075605.
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Author notes
- Eric Lubeck and Ahmet F Coskun: These authors contributed equally to this work.
Authors and Affiliations
- Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA
Eric Lubeck, Ahmet F Coskun, Timur Zhiyentayev, Mubhij Ahmad & Long Cai
Authors
- Eric Lubeck
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Correspondence toLong Cai.
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Lubeck, E., Coskun, A., Zhiyentayev, T. et al. Single-cell in situ RNA profiling by sequential hybridization.Nat Methods 11, 360–361 (2014). https://doi.org/10.1038/nmeth.2892
- Published: 28 March 2014
- Issue Date: April 2014
- DOI: https://doi.org/10.1038/nmeth.2892