Suitability of hCMV for viral gene expression in the brain (original) (raw)

Nature Methods volume 4, page 379 (2007)Cite this article

To the editor:

Viral gene delivery is a powerful tool that is rapidly gaining popularity in experimental neuroscience. I believe that the near future will see rapid expansion of this remarkably versatile and adaptable method. Recent communication by Wickersham et al.1 describes yet another interesting implementation of this technology for retrograde tracing of neurons from their target areas. The key point of the paper is demonstration of a high level of enhanced GFP (EGFP) expression using a rabies virus mutant with glycoprotein gene substituted by EGFP. After injecting the vector into the thalamus, the authors showed many bright EGFP-expressing cortical pyramidal neurons. As a reference, they used a lentivirus with rabies virus envelope glycoprotein in which EGFP expression was driven by human cytomegalovirus (hCMV) promoter and found a very low level of EGFP expression with it.

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References

  1. Wickersham, I.R., Finke, S., Conzelmann, K.K. & Callaway, E.M. Nat. Methods 4, 47–49 (2007).
    Article CAS PubMed Google Scholar
  2. Lonergan, T., Teschemacher, A.G., Hwang, D.-Y., Kim, K.-S. & Kasparov, S. Physiol. Genom. 20, 165–172 (2005).
    Article CAS Google Scholar
  3. Allen, A.M. et al. Hypertension 47, 1054–1061 (2006).
    Article CAS PubMed Google Scholar
  4. Wang, C.Y. & Wang, S. Gene Ther. 13, 1447–1456 (2006).
    Article CAS PubMed Google Scholar

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Authors and Affiliations

  1. University of Bristol, Bristol, BS8 1TD, UK
    Sergey Kasparov

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The author declares no competing financial interests.

Supplementary information

Supplementary Fig. 1

Adenoviral vectors containing hCMV-EGFP expression cassette injected into the nucleus tractus solitarius (NTS) of rats. (PDF 135 kb)

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Kasparov, S. Suitability of hCMV for viral gene expression in the brain.Nat Methods 4, 379 (2007). https://doi.org/10.1038/nmeth0507-379a

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