Rapidly denoising pyrosequencing amplicon reads by exploiting rank-abundance distributions (original) (raw)

Nature Methods volume 7, pages 668–669 (2010)Cite this article

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Pyrosequencing has revolutionized microbial community analysis by allowing the simultaneous assessment of hundreds of microbial communities in multiplex with sufficient depth to resolve meaningful biological patterns. These techniques have been used to study microbial processes on scales ranging from continents1 to within an individual's body2.

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Figure 1: Comparisons of diversity analyses of filtered to denoised data.

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Acknowledgements

We thank P. Turnbaugh for providing us with an excellent mock community for testing and C. Quince for unpublished insights into how PyroNoise works. J.R. was supported in part by a postdoctoral scholarship from the German Academic Exchange Service (DAAD). This work was supported in part by grants from the US National Institutes of Health, the National Aeronautics and Space Administration and by Howard Hughes Medical Institute.

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Authors and Affiliations

  1. Department of Chemistry & Biochemistry, University of Colorado at Boulder, Boulder, Colorado, USA
    Jens Reeder & Rob Knight
  2. Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado, USA
    Rob Knight

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  1. Jens Reeder
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  2. Rob Knight
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Correspondence toRob Knight.

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The authors declare no competing financial interests.

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Reeder, J., Knight, R. Rapidly denoising pyrosequencing amplicon reads by exploiting rank-abundance distributions.Nat Methods 7, 668–669 (2010). https://doi.org/10.1038/nmeth0910-668b

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