A Tn7-based broad-range bacterial cloning and expression system (original) (raw)

References

  1. Craig, N.L. in Mobile DNA (eds. Berg, D.E. & Howe, M.M.) 211–225 (American Society for Microbiology, Washington, D.C., 1989).
    Google Scholar
  2. Craig, N.L. Transposon Tn_7_. Curr. Top. Microbiol. Immunol. 204, 27–48 (1996).
    CAS PubMed Google Scholar
  3. Peters, J.E. & Craig, N.L. Tn_7_: smarter than we thought. Nat. Rev. Mol. Cell Biol. 2, 806–814 (2001).
    Article CAS PubMed Google Scholar
  4. McKown, R.L., Orle, K.A., Chen, T. & Craig, N.L. Sequence requirements of Escherichia coli att_Tn_7, a specific site of transposon Tn_7_ insertion. J. Bacteriol. 170, 352–358 (1988).
    Article CAS PubMed PubMed Central Google Scholar
  5. Hoang, T.T., Karkhoff-Schweizer, R.R., Kutchma, A.J. & Schweizer, H.P. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212, 77–86 (1998).
    Article CAS PubMed Google Scholar
  6. Hojberg, O., Schnider, U., Winteler, H.V., Sorensen, J. & Haas, D. Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil. Appl. Environ. Microbiol. 65, 4085–4093 (1999).
    CAS PubMed PubMed Central Google Scholar
  7. Bao, Y., Lies, D.P., Fu, H. & Roberts, G.P. An improved Tn_7_-based system for the single-copy insertion of cloned genes into the chromosomes of Gram-negative bacteria. Gene 109, 167–168 (1991).
    Article CAS PubMed Google Scholar
  8. Klausen, M. et al. Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. Mol. Microbiol. 48, 1511–1524 (2003).
    Article CAS PubMed Google Scholar
  9. Koch, B., Jensen, L.E. & Nybroe, O. A panel of Tn_7_-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria. J. Microbiol. Methods 45, 187–195 (2001).
    Article CAS PubMed Google Scholar
  10. Miller, V.L. & Mekalanos, J.J. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170, 2575–2583 (1988).
    Article CAS PubMed PubMed Central Google Scholar
  11. Staley, T.E., Lawrence, E.G. & Drahos, D.J. Variable specificity of Tn_7_::lacZY insertion into the chromosome of root-colonizing Pseudomonas putida strains. Mol. Ecol. 6, 85–87 (1997).
    Article CAS Google Scholar
  12. Center for Disease Control and Prevention. Biological and chemical terrorism: strategic plan for preparedness and response. Recommendations of the CDC strategic planning workgroup. Morb. Mortal. Wkly. Rep. 49, 1–4 (2000).
  13. Dance, D.A.B. in Manson's Tropical Diseases (ed. Cook, G.C.) 925–930 (W.B. Saunders Company Ltd., London, 1996).
    Google Scholar
  14. Howe, C. in The Oxford Medicine (ed. Christian, H.A.) 185–202 (Oxford University Press, New York, 1950).
    Google Scholar
  15. Schweizer, H.P. & de Lorenzo, V. in The Pseudomonads - Genomics, life style and molecular architecture (ed. Ramos, J.L.) 317–350 (Kluwer Academic/Plenum, New York, 2004).
    Google Scholar
  16. Schweizer, H.P. A method for construction of bacterial hosts for _lac_-based cloning and expression vectors: α complementation and regulated expression. Biotechniques 17, 452–456 (1994).
    CAS PubMed Google Scholar
  17. Karkhoff-Schweizer, R.R. & Schweizer, H.P. Utilization of mini-D_lac_ transposable element to create an alpha-complementation and regulated expression system for molecular cloning in Pseudomonas aeruginosa. Gene 140, 7–15 (1994).
    Article CAS PubMed Google Scholar
  18. Trieu-Cuot, P., Carlier, C., Poyart-Salmeron, C. & Courvalin, P. An integrative vector exploiting the transposition properties of Tn_1545_ for insertional mutagenesis and cloning of genes from gram-positive bacteria. Gene 106, 21–27 (1991).
    Article CAS PubMed Google Scholar
  19. Stover, C.K. et al. New use of BCG for recombinant vaccines. Nature 351, 456–460 (1991).
    Article CAS PubMed Google Scholar
  20. Hoang, T.T., Kutchma, A.J., Becher, A. & Schweizer, H.P. Integration proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains. Plasmid 43, 59–72 (2000).
    Article CAS PubMed Google Scholar
  21. Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. & Hopwood, D.A. Practical Streptomyces genetics (The John Innes Foundation, Colney, Norwich, UK, 2000).
    Google Scholar
  22. Charpentier, E. et al. Novel cassette-based shuttle vector system for Gram-positive bacteria. Appl. Environ. Microbiol. 70, 6076–6085 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  23. de Lorenzo, V., Herrero, M., Jakubzik, U. & Timmis, K.N. Mini-Tn_5_ transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative bacteria. J. Bacteriol. 172, 6568–6572 (1990).
    Article CAS PubMed PubMed Central Google Scholar
  24. Stellwagen, A.E. & Craig, N.L. Avoiding self: two Tn_7_-encoded proteins mediate target immunity in Tn_7_ transposition. EMBO J. 16, 6823–6834 (1997).
    Article CAS PubMed PubMed Central Google Scholar
  25. Holloway, B.W. Genetic recombination in Pseudomonas aeruginosa. J. Gen. Microbiol. 13, 572–581 (1955).
    CAS PubMed Google Scholar
  26. Chuanchuen, R. et al. Cross-resistance between triclosan and antibiotics in Pseudomonas aeruginosa is mediated by multidrug efflux pumps: exposure of a susceptible strain to triclosan selects nfxB mutants overexpressing MexCD-OprJ. Antimicrob. Agents Chemother. 45, 428–432 (2001).
    Article CAS PubMed PubMed Central Google Scholar
  27. Brett, P.J., DeShazer, D. & Woods, D.E. Burkholderia thailandensis sp. nov., a _Burkholderia pseudomallei_-like species. Int. J. Syst. Bacteriol. 48, 317–320 (1998).
    Article CAS PubMed Google Scholar
  28. De Kievit, T.R., Gillis, R., Marx, S., Brown, C. & Iglewski, B.H. Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Appl. Environ. Microbiol. 67, 1865–1873 (2001).
    Article CAS PubMed PubMed Central Google Scholar
  29. Sambrook, J. & Russell, D.W. Molecular Cloning (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2001).
    Google Scholar
  30. Figurski, D.H. & Helinski, D.R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76, 1648–1652 (1979).
    Article CAS PubMed PubMed Central Google Scholar

Download references