Nonblinking and long-lasting single-molecule fluorescence imaging (original) (raw)
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- Published: 01 October 2006
Nature Methods volume 3, pages 891–893 (2006)Cite this article
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Abstract
Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.
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Acknowledgements
We thank J. Liu for introducing Trolox to us and the US National Institutes of Health for grants.
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Authors and Affiliations
- Department of Physics, Emory University, Atlanta, 30322, Georgia, USA
Ivan Rasnik - Department of Physics, University of Illinois, Urbana, 61801, Illinois, USA
Sean A McKinney & Taekjip Ha - Howard Hughes Medical Institute, Urbana, 61801, Illinois, USA
Taekjip Ha
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- Ivan Rasnik
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Correspondence toIvan Rasnik.
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Rasnik, I., McKinney, S. & Ha, T. Nonblinking and long-lasting single-molecule fluorescence imaging.Nat Methods 3, 891–893 (2006). https://doi.org/10.1038/nmeth934
- Received: 28 April 2006
- Accepted: 11 August 2006
- Published: 01 October 2006
- Issue Date: 01 November 2006
- DOI: https://doi.org/10.1038/nmeth934