Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF (original) (raw)

Nature Neuroscience volume 11, pages 131–133 (2008)Cite this article

Abstract

Pro- and mature BDNF activate very different receptors and intracellular pathways, potentially leading to either neuronal death or survival. Here we examined the biochemistry of endogenous BDNF in mouse neurons using sensitive reagents and found that pro-BDNF is rapidly converted intracellularly to mature BDNF, the latter being stored and released by excitatory input.

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Acknowledgements

We thank S. Wiese and M. Sendtner for the floxed BDNF line and the BDNF antibodies, B. Fayard for constructs, and V. Crotet for technical assistance. T.M. was supported by the Uehara Memorial Foundation and by the Mochida Memorial Foundation for Medical and Pharmaceutical Research and M.K. by the Deutsche Forschungsgemeinschaft.

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Author notes

  1. Johannes Klose
    Present address: Present addresses: Allianz Risk Transfer, Tödistrasse 61, CH-8002 Zürich, Switzerland.,

Authors and Affiliations

  1. Biozentrum, University of Basel, Klingelbergstrasse 50/70, Basel, CH-4056, Switzerland
    Tomoya Matsumoto, Stefanie Rauskolb & Yves-Alain Barde
  2. Technical University Braunschweig, Mendelssohnstrasse 4, Braunschweig, D-38106, Germany
    Martin Polack & Martin Korte
  3. Max-Planck-Institute of Neurobiology, Martinsried, D-82152, Germany
    Johannes Klose, Roland Kolbeck & Yves-Alain Barde
  4. MedImmune, One MedImmune Way, Gaithersburg, 20878, Maryland, USA
    Roland Kolbeck

Authors

  1. Tomoya Matsumoto
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  2. Stefanie Rauskolb
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  3. Martin Polack
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  4. Johannes Klose
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  5. Roland Kolbeck
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  6. Martin Korte
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  7. Yves-Alain Barde
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Contributions

S.R. generated the c_Bdnf_ mutant mice. J.K. and R.K. generated the Bdnf-Myc mutant mice. M.P. and M.K. carried out the electrophysiology. T.M. and Y.-A.B. designed and carried out all of the other experiments, and wrote the manuscript.

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Correspondence toYves-Alain Barde.

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Matsumoto, T., Rauskolb, S., Polack, M. et al. Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.Nat Neurosci 11, 131–133 (2008). https://doi.org/10.1038/nn2038

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