Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF (original) (raw)
- Brief Communication
- Published: 20 January 2008
- Stefanie Rauskolb1,
- Martin Polack2,
- Johannes Klose3 nAff6,
- Roland Kolbeck3,4,
- Martin Korte2 &
- …
- Yves-Alain Barde1,3
Nature Neuroscience volume 11, pages 131–133 (2008)Cite this article
- 5255 Accesses
- 276 Citations
- Metrics details
Abstract
Pro- and mature BDNF activate very different receptors and intracellular pathways, potentially leading to either neuronal death or survival. Here we examined the biochemistry of endogenous BDNF in mouse neurons using sensitive reagents and found that pro-BDNF is rapidly converted intracellularly to mature BDNF, the latter being stored and released by excitatory input.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Lu, B., Pang, P.T. & Woo, N.H. Nat. Rev. Neurosci. 6, 603–614 (2005).
Article CAS Google Scholar - Barde, Y.A., Edgar, D. & Thoenen, H. EMBO J. 1, 549–553 (1982).
Article CAS Google Scholar - Mowla, S.J. et al. J. Biol. Chem. 276, 12660–12666 (2001).
Article CAS Google Scholar - Kolbeck, R., Bartke, I., Eberle, W. & Barde, Y.A. J. Neurochem. 72, 1930–1938 (1999).
Article CAS Google Scholar - Hardingham, G.E., Fukunaga, Y. & Bading, H. Nat. Neurosci. 5, 405–414 (2002).
Article CAS Google Scholar - Woo, N.H. et al. Nat. Neurosci. 8, 1069–1077 (2005).
Article CAS Google Scholar - Rosch, H., Schweigreiter, R., Bonhoeffer, T., Barde, Y.A. & Korte, M. Proc. Natl. Acad. Sci. USA 102, 7362–7367 (2005).
Article Google Scholar - Teng, H.K. et al. J. Neurosci. 25, 5455–5463 (2005).
Article CAS Google Scholar - Zagrebelsky, M. et al. J. Neurosci. 25, 9989–9999 (2005).
Article CAS Google Scholar - Poo, M.M. Nat. Rev. Neurosci. 2, 24–32 (2001).
Article CAS Google Scholar - Harrington, A.W. et al. Proc. Natl. Acad. Sci. USA 101, 6226–6230 (2004).
Article CAS Google Scholar - Egan, M.F. et al. Cell 112, 257–269 (2003).
Article CAS Google Scholar - Chen, Z.Y. et al. Science 314, 140–143 (2006).
Article CAS Google Scholar - Korte, M. et al. Proc. Natl. Acad. Sci. USA 92, 8856–8860 (1995).
Article CAS Google Scholar - Gray, J. et al. Diabetes 55, 3366–3371 (2006).
Article CAS Google Scholar
Acknowledgements
We thank S. Wiese and M. Sendtner for the floxed BDNF line and the BDNF antibodies, B. Fayard for constructs, and V. Crotet for technical assistance. T.M. was supported by the Uehara Memorial Foundation and by the Mochida Memorial Foundation for Medical and Pharmaceutical Research and M.K. by the Deutsche Forschungsgemeinschaft.
Author information
Author notes
- Johannes Klose
Present address: Present addresses: Allianz Risk Transfer, Tödistrasse 61, CH-8002 Zürich, Switzerland.,
Authors and Affiliations
- Biozentrum, University of Basel, Klingelbergstrasse 50/70, Basel, CH-4056, Switzerland
Tomoya Matsumoto, Stefanie Rauskolb & Yves-Alain Barde - Technical University Braunschweig, Mendelssohnstrasse 4, Braunschweig, D-38106, Germany
Martin Polack & Martin Korte - Max-Planck-Institute of Neurobiology, Martinsried, D-82152, Germany
Johannes Klose, Roland Kolbeck & Yves-Alain Barde - MedImmune, One MedImmune Way, Gaithersburg, 20878, Maryland, USA
Roland Kolbeck
Authors
- Tomoya Matsumoto
You can also search for this author inPubMed Google Scholar - Stefanie Rauskolb
You can also search for this author inPubMed Google Scholar - Martin Polack
You can also search for this author inPubMed Google Scholar - Johannes Klose
You can also search for this author inPubMed Google Scholar - Roland Kolbeck
You can also search for this author inPubMed Google Scholar - Martin Korte
You can also search for this author inPubMed Google Scholar - Yves-Alain Barde
You can also search for this author inPubMed Google Scholar
Contributions
S.R. generated the c_Bdnf_ mutant mice. J.K. and R.K. generated the Bdnf-Myc mutant mice. M.P. and M.K. carried out the electrophysiology. T.M. and Y.-A.B. designed and carried out all of the other experiments, and wrote the manuscript.
Corresponding author
Correspondence toYves-Alain Barde.
Supplementary information
Rights and permissions
About this article
Cite this article
Matsumoto, T., Rauskolb, S., Polack, M. et al. Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.Nat Neurosci 11, 131–133 (2008). https://doi.org/10.1038/nn2038
- Received: 13 November 2007
- Accepted: 12 December 2007
- Published: 20 January 2008
- Issue Date: February 2008
- DOI: https://doi.org/10.1038/nn2038