Color-coded fluorescence imaging of tumor-host interactions (original) (raw)

Nature Protocols volume 1, pages 928–935 (2006)Cite this article

Abstract

Fluorescent proteins have the properties of being very bright with high quantum yield and are available in many colors. Tumor-host models consist of transgenic mice expressing green fluorescent protein (GFP) in essentially all cells and tissues or expressing GFP selectively in specific tissues such as blood vessels. Particularly useful are the corresponding nude mice transgenic for GFP expression, as they can accept human tumors. When tumor cells expressing red fluorescent protein are implanted in mice expressing GFP, various types of tumor-host interactions can be observed, including those involving host blood vessels, lymphocytes, tumor-associated fibroblasts, macrophages, dendritic cells and others. The 'color-coded' tumor-host models enable imaging and therefore a deeper understanding of the host cells involved and their function in tumor progression. Approximately 4–8 weeks are needed for these procedures.

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Authors and Affiliations

  1. AntiCancer, 7917 Ostrow Street, San Diego, 92111, California, USA
    Robert M Hoffman & Meng Yang
  2. Department of Surgery, University of California, San Diego, 200 West Arbor Drive, San Diego, 92103-8220, California, USA
    Robert M Hoffman

Authors

  1. Robert M Hoffman
  2. Meng Yang

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Correspondence toRobert M Hoffman.

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Competing interests

R.M.H. is the president of AntiCancer, which has commercial activities in the area of fluorescent protein–based imaging and has a Technology Development with Olympus.

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Hoffman, R., Yang, M. Color-coded fluorescence imaging of tumor-host interactions.Nat Protoc 1, 928–935 (2006). https://doi.org/10.1038/nprot.2006.119

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