Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy (original) (raw)

• Fridovich, I. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64, 97–112 (1995).
  Article CAS PubMed Google Scholar
• Griendling, K.K., Sorescu, D. & Ushio-Fukai, M. NAD(P)H oxidase: role in cardiovascular biology and disease. Circ. Res. 86, 494–501 (2000).
  Article CAS PubMed Google Scholar
• Csiszar, A., Pacher, P., Kaley, G. & Ungvari, Z. Role of oxidative and nitrosative stress, longevity genes and poly(ADP-ribose) polymerase in cardiovascular dysfunction associated with aging. Curr. Vasc. Pharmacol. 3, 285–291 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Pacher, P., Nivorozhkin, A. & Szabo, C. Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacol. Rev. 58, 87–114 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Pacher, P., Beckman, J.S. & Liaudet, L. Nitric oxide and peroxynitrite in health and disease. Physiol. Rev. 87, 315–424 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Gomes, A., Fernandes, E. & Lima, J.L. Fluorescence probes used for detection of reactive oxygen species. J. Biochem. Biophys. Methods 65, 45–80 (2005).
  Article CAS PubMed Google Scholar
• Robinson, K.M. et al. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc. Natl. Acad. Sci. USA 103, 15038–15043 (2006).
  Article CAS PubMed Google Scholar
• Soh, N. Recent advances in fluorescent probes for the detection of reactive oxygen species. Anal. Bioanal. Chem. 386, 532–543 (2006).
  Article CAS PubMed Google Scholar
• Zhao, H. et al. Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide. Free Radic. Biol. Med. 34, 1359–1368 (2003).
  Article CAS PubMed Google Scholar
• Zhao, H. et al. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc. Natl. Acad. Sci. USA 102, 5727–5732 (2005).
  Article CAS PubMed Google Scholar
• Rezvani, H.R. et al. Hypoxia-inducible factor-1alpha, a key factor in the keratinocyte response to UVB exposure. J. Biol. Chem. 282, 16413–16422 (2007).
  Article CAS PubMed Google Scholar
• Pehar, M. et al. Mitochondrial superoxide production and nuclear factor erythroid 2-related factor 2 activation in p75 neurotrophin receptor-induced motor neuron apoptosis. J. Neurosci. 27, 7777–7785 (2007).
  Article CAS PubMed Google Scholar
• Johnson-Cadwell, L.I., Jekabsons, M.B., Wang, A., Polster, B.M. & Nicholls, D.G. 'Mild uncoupling' does not decrease mitochondrial superoxide levels in cultured cerebellar granule neurons but decreases spare respiratory capacity and increases toxicity to glutamate and oxidative stress. J. Neurochem. 101, 1619–1631 (2007).
  Article CAS PubMed Google Scholar
• Abramov, A.Y., Scorziello, A. & Duchen, M.R. Three distinct mechanisms generate oxygen free radicals in neurons and contribute to cell death during anoxia and reoxygenation. J. Neurosci. 27, 1129–1138 (2007).
  Article CAS PubMed Google Scholar
• Zimmerman, M.C., Oberley, L.W. & Flanagan, S.W. Mutant SOD1-induced neuronal toxicity is mediated by increased mitochondrial superoxide levels. J. Neurochem. (2007).
• Fauconnier, J. et al. Effects of palmitate on Ca2+ handling in adult control and ob/ob cardiomyocytes: impact of mitochondrial reactive oxygen species. Diabetes 56, 1136–1142 (2007).
  Article CAS PubMed Google Scholar
• Iuso, A. et al. Dysfunctions of cellular oxidative metabolism in patients with mutations in the NDUFS1 and NDUFS4 genes of complex I. J. Biol. Chem. 281, 10374–10380 (2006).
  Article CAS PubMed Google Scholar
• Hawkins, B.J., Madesh, M., Kirkpatrick, C.J. & Fisher, A.B. Superoxide flux in endothelial cells via the chloride channel-3 mediates intracellular signaling. Mol. Biol. Cell 18, 2002–2012 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Mukhopadhyay, P., Rajesh, M., Yoshihiro, K., Hasko, G. & Pacher, P. Simple quantitative detection of mitochondrial superoxide production in live cells. Biochem. Biophys. Res. Commun. 358, 203–208 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Brookes, P.S. et al. The triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid and its derivatives elicit human lymphoid cell apoptosis through a novel pathway involving the unregulated mitochondrial permeability transition pore. Cancer Res. 67, 1793–1802 (2007).
  Article CAS PubMed Google Scholar
• Zhang, Y., Soboloff, J., Zhu, Z. & Berger, S.A. Inhibition of Ca2+ influx is required for mitochondrial reactive oxygen species-induced endoplasmic reticulum Ca2+ depletion and cell death in leukemia cells. Mol. Pharmacol. 70, 1424–1434 (2006).
  Article CAS PubMed Google Scholar
• Lund, K.C., Peterson, L.L. & Wallace, K.B. The absence of a universal mechanism of mitochondrial toxicity by nucleoside analogs. Antimicrob. Agents Chemother. (2007).
• Payne, C.M. et al. Deoxycholate induces mitochondrial oxidative stress and activates NF-{kappa}B through multiple mechanisms in HCT-116 colon epithelial cells. Carcinogenesis 28, 215–222 (2007).
  Article CAS PubMed Google Scholar
• Dvorak, K. et al. Bile acids in combination with low pH induce oxidative stress and oxidative DNA damage: relevance to the pathogenesis of Barrett's oesophagus. Gut 56, 763–771 (2007).
  Article CAS PubMed Google Scholar
• Rajesh, M. et al. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am. J. Physiol. Heart Circ. Physiol. 293, H610–H619 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Ungvari, Z. et al. Increased mitochondrial H2O2 production promotes endothelial NF-κB activation in aged rat arteries. Am. J. Physiol. Heart Circ. Physiol. 293, H37–H47 (2007).
  Article CAS PubMed Google Scholar
• Poot, M., Gibson, L.L. & Singer, V.L. Detection of apoptosis in live cells by MitoTracker red CMXRos and SYTO dye flow cytometry. Cytometry 27, 358–364 (1997).
  Article CAS PubMed Google Scholar
• van Genderen, H. et al. In vitro measurement of cell death with the annexin A5 affinity assay. Nat. Protoc. 1, 363–367 (2006).
  Article CAS PubMed Google Scholar
• Mukhopadhyay, P. et al. Pharmacological inhibition of CB1 cannabinoid receptor protects against doxorubicin-induced cardiotoxicity. J. Am. Coll. Cardiol. 50, 528–536 (2007).
  Article CAS PubMed PubMed Central Google Scholar