A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (original) (raw)
Fuchs, E. & Segre, J.A. Stem cells: a new lease on life. Cell100, 143–155 (2000). ArticleCAS Google Scholar
Pittenger, M.F. et al. Multilineage potential of adult human mesenchymal stem cells. Science284, 143–147 (1999). ArticleCAS Google Scholar
Friedenstein, A.J., Gorskaja, J.F. & Kulagina, N.N. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp. Hematol.4, 267–274 (1976). CASPubMed Google Scholar
Bianco, P., Riminucci, M., Gronthos, S. & Robey, P.G. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells19, 180–192 (2001). ArticleCAS Google Scholar
Sun, S. et al. Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method. Stem Cells21, 527–535 (2003). ArticleCAS Google Scholar
Prockop, D.J. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science276, 71–74 (1997). ArticleCAS Google Scholar
Baksh, D., Song, L. & Tuan, R.S. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J. Cell Mol. Med.8, 301–316 (2004). ArticleCAS Google Scholar
Horwitz, E.M. et al. Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: implications for cell therapy of bone. Proc. Natl. Acad. Sci. USA99, 8932–8937 (2002). ArticleCAS Google Scholar
Koc, O.N. et al. Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy. J. Clin. Oncol.18, 307–316 (2000). ArticleCAS Google Scholar
Petite, H. et al. Tissue-engineered bone regeneration. Nat. Biotechnol.18, 959–963 (2000). ArticleCAS Google Scholar
Quarto, R. et al. Repair of large bone defects with the use of autologous bone marrow stromal cells. N. Engl. J. Med.344, 385–386 (2001). ArticleCAS Google Scholar
Grinnemo, K.H. et al. Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium. J. Thorac. Cardiovasc. Surg.127, 1293–1300 (2004). ArticleCAS Google Scholar
Barry, F.P. Mesenchymal stem cell therapy in joint disease. Novartis. Found Symp.249, 86–102, 170–4, 239–41 (2003). PubMed Google Scholar
Peister, A. et al. Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. Blood103, 1662–1668 (2004). ArticleCAS Google Scholar
Phinney, D.G., Kopen, G., Isaacson, R.L. & Prockop, D.J. Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation. J. Cell Biochem.72, 570–585 (1999). ArticleCAS Google Scholar
Baddoo, M. et al. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. J. Cell Biochem.89, 1235–1249 (2003). ArticleCAS Google Scholar
Tropel, P. et al. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp. Cell Res.295, 395–406 (2004). ArticleCAS Google Scholar
Eslaminejad, M.B., Nikmahzar, A., Taghiyar, L., Nadri, S. & Massumi, M. Murine mesenchymal stem cells isolated by low density primary culture system. Dev. Growth Differ.48, 361–370 (2006). ArticleCAS Google Scholar
Nadri, S. & Soleimani, M. Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev. Biol. Anim.43, 276–282 (2007). ArticleCAS Google Scholar
Nadri, S. et al. An efficient method for isolation of murine bone marrow mesenchymal stem cells. Int. J. Dev. Biol.51, 723–729 (2007). ArticleCAS Google Scholar
Modderman, W.E., Vrijheid-Lammers, T., Lowik, C.W. & Nijweide, P.J. Removal of hematopoietic cells and macrophages from mouse bone marrow cultures: isolation of fibroblastlike stromal cells. Exp. Hematol.22, 194–201 (1994). CASPubMed Google Scholar
Falla, N. et al. Characterization of a 5-fluorouracil-enriched osteoprogenitor population of the murine bone marrow. Blood82, 3580–3591 (1993). CASPubMed Google Scholar
Van Vlasselaer, P., Falla, N., Snoeck, H. & Mathieu, E. Characterization and purification of osteogenic cells from murine bone marrow by two-color cell sorting using anti-Sca-1 monoclonal antibody and wheat germ agglutinin. Blood84, 753–763 (1994). CASPubMed Google Scholar
Wang, Q.R. & Wolf, N.S. Dissecting the hematopoietic microenvironment. VIII. Clonal isolation and identification of cell types in murine CFU-F colonies by limiting dilution. Exp. Hematol.18, 355–359 (1990). CASPubMed Google Scholar
Kitano, Y., Radu, A., Shaaban, A. & Flake, A.W. Selection, enrichment, and culture expansion of murine mesenchymal progenitor cells by retroviral transduction of cycling adherent bone marrow cells. Exp. Hematol.28, 1460–1469 (2000). ArticleCAS Google Scholar
Muraglia, A., Cancedda, R. & Quarto, R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J. Cell Sci.113 (Part 7): 1161–1166 (2000). CASPubMed Google Scholar
Digirolamo, C.M. et al. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br. J. Haematol.107, 275–281 (1999). ArticleCAS Google Scholar
Keating, A., Horsfall, W., Hawley, R.G. & Toneguzzo, F. Effect of different promoters on expression of genes introduced into hematopoietic and marrow stromal cells by electroporation. Exp. Hematol.18, 99–102 (1990). CASPubMed Google Scholar
Deans, R.J. & Moseley, A.B. Mesenchymal stem cells: biology and potential clinical uses. Exp. Hematol.28, 875–884 (2000). ArticleCAS Google Scholar
Otsuka, T. et al. Expression of the c-kit ligand and interleukin 6 genes in mouse bone marrow stromal cell lines. Stem Cells12, 409–415 (1994). ArticleCAS Google Scholar