Castera, L. & Pinzani, M. Non-invasive assessment of liver fibrosis: are we ready? Lancet375, 1419–1420 (2010). ArticlePubMed Google Scholar
Hamer, O. W. et al. Fatty liver: imaging patterns and pitfalls. Radiographics26, 1637–1653 (2006). ArticlePubMed Google Scholar
Schwenzer, N. F. et al. Non-invasive assessment and quantification of liver steatosis by ultrasound, computed tomography and magnetic resonance. J. Hepatol.51, 433–445 (2009). ArticlePubMed Google Scholar
Webb, M. et al. Diagnostic value of a computerized hepatorenal index for sonographic quantification of liver steatosis. AJR Am. J. Roentgenol.192, 909–914 (2009). ArticlePubMed Google Scholar
Ratziu, V., Bellentani, S., Cortez-Pinto, H., Day, C. & Marchesini, G. A position statement on NAFLD/NASH based on the EASL 2009 special conference. J. Hepatol.53, 372–384 (2010). PubMed Google Scholar
Sasso, M. et al. Controlled attenuation parameter (CAP): a novel VCTE guided ultrasonic attenuation measurement for the evaluation of hepatic steatosis: preliminary study and validation in a cohort of patients with chronic liver disease from various causes. Ultrasound Med. Biol.36, 1825–1835 (2010). ArticlePubMed Google Scholar
de Ledinghen, V., Vergniol, J., Foucher, J., Merrouche W. & le Bail, B. Non-invasive diagnosis of liver steatosis using controlled attenuation parameter (CAP) and transient elastography. Liver Int.32, 911–918 (2012). ArticlePubMed Google Scholar
Myers, R. P. et al. Controlled Attenuation Parameter (CAP): a noninvasive method for the detection of hepatic steatosis based on transient elastography. Liver Int.32, 902–910 (2012). ArticlePubMed Google Scholar
Sasso, M. et al. Novel controlled attenuation parameter for noninvasive assessment of steatosis using Fibroscan((R.)): validation in chronic hepatitis C. J. Viral Hepat.19, 244–253 (2012). ArticleCASPubMed Google Scholar
Friedrich-Rust, M. et al. Acoustic radiation force impulse-imaging and transient elastography for non-invasive assessment of liver fibrosis and steatosis in NAFLD. Eur. J. Radiol.81, e325–e331 (2012). ArticlePubMed Google Scholar
Masaki, K. et al. Utility of controlled attenuation parameter measurement for assessing liver steatosis in Japanese patients with chronic liver diseases. Hepatol. Res.http://dx.doi.org/10.1111/hepr.12094.
Saadeh, S. et al. The utility of radiological imaging in nonalcoholic fatty liver disease. Gastroenterology123, 745–750 (2002). ArticlePubMed Google Scholar
Szczepaniak, L. S. et al. Magnetic resonance spectroscopy to measure hepatic triglyceride content: prevalence of hepatic steatosis in the general population. Am. J. Physiol. Endocrinol. Metab.288, E462–E468 (2005). ArticleCASPubMed Google Scholar
Wong, V. W. et al. Prevalence of non-alcoholic fatty liver disease and advanced fibrosis in Hong Kong Chinese: a population study using proton-magnetic resonance spectroscopy and transient elastography. Gut61, 409–415 (2012). ArticlePubMed Google Scholar
Guiu, B. et al. Quantification of liver fat content: comparison of triple-echo chemical shift gradient-echo imaging and in vivo proton MR spectroscopy. Radiology250, 95–102 (2009). ArticlePubMed Google Scholar
Lee, S. S. et al. Non-invasive assessment of hepatic steatosis: prospective comparison of the accuracy of imaging examinations. J. Hepatol.52, 579–585 (2010). ArticleCASPubMed Google Scholar
van Werven, J. R. et al. Assessment of hepatic steatosis in patients undergoing liver resection: comparison of US, CT, T1-weighted dual-echo MR imaging, and point-resolved 1H MR spectroscopy. Radiology256, 159–168 (2010). ArticlePubMed Google Scholar
Bedogni, G. et al. The Fatty Liver Index: a simple and accurate predictor of hepatic steatosis in the general population. BMC Gastroenterol.6, 33 (2006). ArticleCASPubMedPubMed Central Google Scholar
Bedogni, G., Kahn, H. S., Bellentani, S. & Tiribelli, C. A simple index of lipid overaccumulation is a good marker of liver steatosis. BMC Gastroenterol.10, 98 (2010). ArticleCASPubMedPubMed Central Google Scholar
Kotronen, A. et al. Prediction of non-alcoholic fatty liver disease and liver fat using metabolic and genetic factors. Gastroenterology137, 865–872 (2009). ArticleCASPubMed Google Scholar
Lee, J. H. et al. Hepatic steatosis index: a simple screening tool reflecting nonalcoholic fatty liver disease. Dig. Liver Dis.42, 503–508 (2010). ArticleCASPubMed Google Scholar
Dam-Larsen, S. et al. Final results of a long-term, clinical follow-up in fatty liver patients. Scand. J. Gastroenterol.44, 1236–1243 (2009). ArticleCASPubMed Google Scholar
Ekstedt, M. et al. Long-term follow-up of patients with NAFLD and elevated liver enzymes. Hepatology44, 865–873 (2006). ArticleCASPubMed Google Scholar
Angulo, P. Long-term mortality in nonalcoholic fatty liver disease: is liver histology of any prognostic significance? Hepatology51, 373–375 (2010). ArticlePubMed Google Scholar
Younossi, Z. M. et al. Pathologic criteria for nonalcoholic steatohepatitis: interprotocol agreement and ability to predict liver-related mortality. Hepatology53, 1874–1882 (2011). ArticlePubMed Google Scholar
Zardi, E. M. et al. Which clinical and sonographic parameters may be useful to discriminate NASH from steatosis? J. Clin. Gastroenterol.45, 59–63 (2011). ArticlePubMed Google Scholar
Suzuki, K. et al. Measurement of spleen volume is useful for distinguishing between simple steatosis and early-stage non-alcoholic steatohepatitis. Hepatol Res.40, 693–700 (2010). ArticleCASPubMed Google Scholar
Chen, J. et al. Early detection of nonalcoholic steatohepatitis in patients with nonalcoholic fatty liver disease by using MR elastography. Radiology259, 749–756 (2011). ArticlePubMedPubMed Central Google Scholar
Poynard, T. et al. Diagnostic value of biochemical markers (NashTest) for the prediction of non alcoholo steato hepatitis in patients with non-alcoholic fatty liver disease. BMC Gastroenterol.6, 34 (2006). ArticleCASPubMedPubMed Central Google Scholar
Palekar, N. A., Naus, R., Larson, S. P., Ward, J. & Harrison, S. A. Clinical model for distinguishing nonalcoholic steatohepatitis from simple steatosis in patients with nonalcoholic fatty liver disease. Liver Int.26, 151–156 (2006). ArticlePubMed Google Scholar
Shimada, M. et al. Usefulness of a combined evaluation of the serum adiponectin level, HOMA-IR, and serum type IV collagen 7S level to predict the early stage of nonalcoholic steatohepatitis. Am. J. Gastroenterol.102, 1931–1938 (2007). ArticleCASPubMed Google Scholar
Wieckowska, A. et al. In vivo assessment of liver cell apoptosis as a novel biomarker of disease severity in nonalcoholic fatty liver disease. Hepatology44, 27–33 (2006). ArticleCASPubMed Google Scholar
Feldstein, A. E. et al. Cytokeratin-18 fragment levels as noninvasive biomarkers for nonalcoholic steatohepatitis: a multicenter validation study. Hepatology50, 1072–1078 (2009). ArticleCASPubMed Google Scholar
Alkhouri, N., Carter-Kent, C. & Feldstein, A. E. Apoptosis in nonalcoholic fatty liver disease: diagnostic and therapeutic implications. Expert Rev. Gastroenterol. Hepatol.5, 201–212 (2011). ArticlePubMedPubMed Central Google Scholar
Shen, J. et al. Non-invasive diagnosis of non-alcoholic steatohepatitis by combined serum biomarkers. J. Hepatol.56, 1363–1370 (2012). ArticleCASPubMed Google Scholar
Sandrin, L. et al. Transient elastography: a new noninvasive method for assessment of hepatic fibrosis. Ultrasound Med. Biol.29, 1705–1713 (2003). ArticlePubMed Google Scholar
Roulot, D. et al. Liver stiffness values in apparently healthy subjects: Influence of gender and metabolic syndrome. J. Hepatol.48, 606–613 (2008). ArticlePubMed Google Scholar
Castera, L., Forns, X. & Alberti, A. Non-invasive evaluation of liver fibrosis using transient elastography. J. Hepatol.48, 835–847 (2008). ArticlePubMed Google Scholar
Castera, L. Noninvasive methods to assess liver disease in patients with hepatitis B or C. Gastroenterology142, 1293–1302.e4 (2012). ArticlePubMed Google Scholar
Yoneda, M. et al. Noninvasive assessment of liver fibrosis by measurement of stiffness in patients with nonalcoholic fatty liver disease (NAFLD). Dig. Liver Dis.40, 371–378 (2008). ArticleCASPubMed Google Scholar
Nobili, V. et al. Accuracy and reproducibility of transient elastography for the diagnosis of fibrosis in pediatric nonalcoholic steatohepatitis. Hepatology48, 442–448 (2008). ArticleCASPubMed Google Scholar
Wong, V. W. et al. Diagnosis of fibrosis and cirrhosis using liver stiffness measurement in nonalcoholic fatty liver disease. Hepatology51, 454–462 (2010). ArticleCASPubMed Google Scholar
Lupsor, M. et al. Performance of unidimensional transient elastography in staging non-alcoholic steatohepatitis. J. Gastrointestin. Liver Dis.19, 53–60 (2010). PubMed Google Scholar
Petta, S. et al. Reliability of liver stiffness measurement in non-alcoholic fatty liver disease: the effects of body mass index. Aliment. Pharmacol. Ther.33, 1350–1360 (2011). ArticleCASPubMed Google Scholar
Gaia, S. et al. Reliability of transient elastography for the detection of fibrosis in non-alcoholic fatty liver disease and chronic viral hepatitis. J. Hepatol.54, 64–71 (2011). ArticlePubMed Google Scholar
Myers, R. P. et al. Feasibility and diagnostic performance of the FibroScan XL probe for liver stiffness measurement in overweight and obese patients. Hepatology55, 199–208 (2012). ArticlePubMed Google Scholar
Wong, V. W. et al. Liver stiffness measurement using XL probe in patients with nonalcoholic fatty liver disease. Am. J. Gastroenterol.107, 1862–1871 (2012). ArticlePubMed Google Scholar
Ransohoff, D. F. & Feinstein, A. R. Problems of spectrum and bias in evaluating the efficacy of diagnostic tests. N. Engl. J. Med.299, 926–930 (1978). ArticleCASPubMed Google Scholar
Poynard, T. et al. Standardization of ROC curve areas for diagnostic evaluation of liver fibrosis markers based on prevalences of fibrosis stages. Clin. Chem.53, 1615–1622 (2007). ArticleCASPubMed Google Scholar
Castera, L. et al. Pitfalls of liver stiffness measurement: A 5-year prospective study of 13,369 examinations. Hepatology51, 828–835 (2010). PubMed Google Scholar
Wong, G. L. et al. Factors associated with unreliable liver stiffness measurement and its failure with transient elastography in the Chinese population. J. Gastroenterol. Hepatol.26, 300–305 (2011). ArticlePubMed Google Scholar
Friedrich-Rust, M. et al. Transient elastography with a new probe for obese patients for non-invasive staging of non-alcoholic steatohepatitis. Eur. Radiol.20, 2390–2396 (2010). ArticlePubMed Google Scholar
de Ledinghen, V. et al. Feasibility of liver transient elastography with FibroScan using a new probe for obese patients. Liver Int.30, 1043–1048 (2010). ArticleCASPubMed Google Scholar
de Ledinghen, V. et al. Diagnosis of liver fibrosis and cirrhosis using liver stiffness measurement: comparison between M and XL probe of FibroScan®. J. Hepatol.56, 833–839 (2012). ArticlePubMed Google Scholar
Coco, B. et al. Transient elastography: a new surrogate marker of liver fibrosis influenced by major changes of transaminases. J. Viral Hepat.14, 360–369 (2007). ArticleCASPubMed Google Scholar
Sagir, A., Erhardt, A., Schmitt, M. & Haussinger, D. Transient elastography is unreliable for detection of cirrhosis in patients with acute liver damage. Hepatology47, 592–595 (2007). ArticleCAS Google Scholar
Arena, U. et al. Acute viral hepatitis increases liver stiffness values measured by transient elastography. Hepatology47, 380–384 (2008). ArticleCASPubMed Google Scholar
Millonig, G. et al. Extrahepatic cholestasis increases liver stiffness (FibroScan) irrespective of fibrosis. Hepatology48, 1718–1723 (2008). ArticlePubMed Google Scholar
Millonig, G. et al. Liver stiffness is directly influenced by central venous pressure. J. Hepatol.52, 206–210 (2010). ArticlePubMed Google Scholar
Mederacke, I. et al. Food intake increases liver stiffness in patients with chronic or resolved hepatitis C virus infection. Liver Int.29, 1500–1506 (2009). ArticlePubMed Google Scholar
Arena, U. et al. Liver stiffness is influenced by a standardized meal in patients with chronic HCV hepatitis at different stages of fibrotic evolution. Hepatology58, 65–72 (2013). ArticleCASPubMed Google Scholar
Berzigotti, A. et al. Effect of meal ingestion on liver stiffness in patients with cirrhosis and portal hypertension. PLoS ONE8, e58742 (2013). ArticleCASPubMedPubMed Central Google Scholar
Berzigotti, A. & Castera, L. Update on Ultrasound Imaging of Liver Fibrosis. J. Hepatol.58, 180–182 (2013). Article Google Scholar
Nightingale, K., Soo, M. S., Nightingale, R. & Trahey, G. Acoustic radiation force impulse imaging: in vivo demonstration of clinical feasibility. Ultrasound Med. Biol.28, 227–235 (2002). ArticlePubMed Google Scholar
Palmeri, M. L. et al. Noninvasive evaluation of hepatic fibrosis using acoustic radiation force-based shear stiffness in patients with nonalcoholic fatty liver disease. J. Hepatol.55, 666–672 (2011). ArticlePubMedPubMed Central Google Scholar
Yoneda, M. et al. Nonalcoholic fatty liver disease: US-based acoustic radiation force impulse elastography. Radiology256, 640–647 (2010). ArticlePubMed Google Scholar
Ochi, H. et al. Real-time tissue elastography for evaluation of hepatic fibrosis and portal hypertension in nonalcoholic fatty liver diseases. Hepatology56, 1271–1278 (2012). ArticlePubMed Google Scholar
Muthupillai, R. et al. Magnetic resonance elastography by direct visualization of propagating acoustic strain waves. Science269, 1854–1857 (1995). ArticleCASPubMed Google Scholar
Talwalkar, J. A. et al. Magnetic resonance imaging of hepatic fibrosis: emerging clinical applications. Hepatology47, 332–342 (2008). ArticlePubMed Google Scholar
Kim, D., Kim, W. R., Talwalkar, J. A., Kim, H. J. & Ehman, R. L. Advanced fibrosis in nonalcoholic fatty liver disease: noninvasive assessment with MR elastography. Radiology268, 411–419 (2013). ArticlePubMedPubMed Central Google Scholar
Huwart, L. et al. Magnetic resonance elastography for the noninvasive staging of liver fibrosis. Gastroenterology135, 32–40 (2008). ArticlePubMed Google Scholar
Sheth, S. G., Flamm, S. L., Gordon, F. D. & Chopra, S. AST/ALT ratio predicts cirrhosis in patients with chronic hepatitis C virus infection. Am. J. Gastroenterol.93, 44–48 (1998). ArticleCASPubMed Google Scholar
Angulo, P., Keach, J. C., Batts, K. P. & Lindor, K. D. Independent predictors of liver fibrosis in patients with nonalcoholic steatohepatitis. Hepatology30, 1356–1362 (1999). ArticleCASPubMed Google Scholar
Kruger, F. C. et al. APRI: a simple bedside marker for advanced fibrosis that can avoid liver biopsy in patients with NAFLD/NASH. S. Afr. Med. J.101, 477–480 (2011). CASPubMed Google Scholar
Chitturi, S. et al. HFE mutations, hepatic iron, and fibrosis: ethnic-specific association of NASH with C282Y but not with fibrotic severity. Hepatology36, 142–149 (2002). ArticleCASPubMed Google Scholar
Bugianesi, E. et al. Relative contribution of iron burden, HFE mutations, and insulin resistance to fibrosis in nonalcoholic fatty liver. Hepatology39, 179–187 (2004). ArticleCASPubMed Google Scholar
Manousou, P. et al. Serum ferritin is a discriminant marker for both fibrosis and inflammation in histologically proven non-alcoholic fatty liver disease patients. Liver Int.31, 730–739 (2011). ArticleCASPubMed Google Scholar
Angulo, P. et al. The NAFLD fibrosis score: a noninvasive system that identifies liver fibrosis in patients with NAFLD. Hepatology45, 846–854 (2007). ArticleCASPubMed Google Scholar
Dixon, J. B., Bhathal, P. S. & O'Brien, P. E. Nonalcoholic fatty liver disease: predictors of nonalcoholic steatohepatitis and liver fibrosis in the severely obese. Gastroenterology121, 91–100 (2001). ArticleCASPubMed Google Scholar
Harrison, S. A., Oliver, D., Arnold, H. L., Gogia, S. & Neuschwander-Tetri, B. A. Development and validation of a simple NAFLD clinical scoring system for identifying patients without advanced disease. Gut57, 1441–1447 (2008). ArticleCASPubMed Google Scholar
Marceau, P. et al. Liver pathology and the metabolic syndrome X in severe obesity. J. Clin. Endocrinol. Metab.84, 1513–1517 (1999). ArticleCASPubMed Google Scholar
Miyaaki, H. et al. Clinicopathological study of nonalcoholic fatty liver disease in Japan: the risk factors for fibrosis. Liver Int.28, 519–524 (2008). ArticlePubMed Google Scholar
Ratziu, V. et al. Liver fibrosis in overweight patients. Gastroenterology118, 1117–1123 (2000). ArticleCASPubMed Google Scholar
Adams, L. A. et al. Complex non-invasive fibrosis models are more accurate than simple models in non-alcoholic fatty liver disease. J. Gastroenterol. Hepatol.26, 1536–1543 (2011). ArticleCASPubMed Google Scholar
Cales, P. et al. Comparison of blood tests for liver fibrosis specfic or not specific to NAFLD. J. Hepatol.50, 165–173 (2009). ArticlePubMed Google Scholar
Shah, A. G. et al. Comparison of noninvasive markers of fibrosis in patients with nonalcoholic fatty liver disease. Clin. Gastroenterol. Hepatol.7, 1104–1112 (2009). ArticleCASPubMedPubMed Central Google Scholar
Adams, L. A. et al. Hepascore: an accurate validated predictor of liver fibrosis in chronic hepatitis C infection. Clin. Chem.51, 1867–1873 (2005). ArticleCASPubMed Google Scholar
Qureshi, K., Clements, R. H. & Abrams, G. A. The utility of the “NAFLD fibrosis score” in morbidly obese subjects with NAFLD. Obes. Surg.18, 264–270 (2008). ArticlePubMed Google Scholar
Wong, V. W. et al. Validation of the NAFLD fibrosis score in a Chinese population with low prevalence of advanced fibrosis. Am. J. Gastroenterol.103, 1682–1688 (2008). ArticlePubMed Google Scholar
Chalasani, N. et al. The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the American Gastroenterological Association, American Association for the Study of Liver Diseases, and American College of Gastroenterology. Gastroenterology142, 1592–1609 (2012). ArticlePubMed Google Scholar
Guha, I. N. et al. Noninvasive markers of fibrosis in nonalcoholic fatty liver disease: Validating the European Liver Fibrosis Panel and exploring simple markers. Hepatology47, 455–460 (2008). ArticlePubMed Google Scholar
Kaneda, H., Hashimoto, E., Yatsuji, S. & Tokushige, K., Shiratori, K. Hyaluronic acid levels can predict severe fibrosis and platelet counts can predict cirrhosis in patients with nonalcoholic fatty liver disease. J. Gastroenterol. Hepatol.21, 1459–1465 (2006). CASPubMed Google Scholar
Nobili, V. et al. Performance of ELF serum markers in predicting fibrosis stage in pediatric non-alcoholic fatty liver disease. Gastroenterology136, 160–167 (2009). ArticleCASPubMed Google Scholar
Ratziu, V. et al. Diagnostic value of biochemical markers (FibroTest-FibroSURE) for the prediction of liver fibrosis in patients with non-alcoholic fatty liver disease. BMC Gastroenterol.6, 6 (2006). ArticleCASPubMedPubMed Central Google Scholar
Sakugawa, H. et al. Clinical usefulness of biochemical markers of liver fibrosis in patients with nonalcoholic fatty liver disease. World J. Gastroenterol.11, 255–259 (2005). ArticleCASPubMedPubMed Central Google Scholar
Santos, V. N. et al. Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease. Braz. J. Med. Biol. Res.38, 747–753 (2005). ArticlePubMed Google Scholar
Suzuki, A. et al. Hyaluronic acid, an accurate serum marker for severe hepatic fibrosis in patients with non-alcoholic fatty liver disease. Liver Int.25, 779–786 (2005). ArticleCASPubMed Google Scholar
Johansen, J. S. et al. Serum YKL-40 is increased in patients with hepatic fibrosis. J. Hepatol.32, 911–920 (2000). ArticleCASPubMed Google Scholar
Malik, R. et al. The clinical utility of biomarkers and the nonalcoholic steatohepatitis CRN liver biopsy scoring system in patients with nonalcoholic fatty liver disease. J. Gastroenterol. Hepatol.24, 564–568 (2009). ArticleCASPubMed Google Scholar
Rosenberg, W. M. et al. Serum markers detect the presence of liver fibrosis: A cohort study. Gastroenterology127, 1704–1713 (2004). ArticlePubMed Google Scholar
Adams, L. Transient elastography in nonalcoholic fatty liver disease: making sense of echoes. Hepatology51, 370–372 (2010). ArticlePubMed Google Scholar
Castera, L. et al. Prospective comparison of transient elastography, Fibrotest, APRI, and liver biopsy for the assessment of fibrosis in chronic hepatitis C. Gastroenterology128, 343–350 (2005). ArticlePubMed Google Scholar
Poynard, T. et al. Concordance in a world without a gold standard: a new non-invasive methodology for improving accuracy of fibrosis markers. PLoS ONE3, e3857 (2008). ArticleCASPubMedPubMed Central Google Scholar
Ngo, Y. et al. A prospective analysis of the prognostic value of biomarkers (FibroTest) in patients with chronic hepatitis C. Clin. Chem.52, 1887–1896 (2006). CASPubMed Google Scholar
Naveau, S. et al. Diagnostic and prognostic values of noninvasive biomarkers of fibrosis in patients with alcoholic liver disease. Hepatology49, 97–105 (2009). ArticlePubMed Google Scholar
Parkes, J. et al. Enhanced liver fibrosis test can predict clinical outcomes in patients with chronic liver disease. Gut59, 1245–1251 (2010). ArticleCASPubMed Google Scholar
Nunes, D. et al. Noninvasive markers of liver fibrosis are highly predictive of liver-related death in a cohort of HCV-infected individuals with and without HIV infection. Am. J. Gastroenterol.105, 1346–1353 (2010). ArticlePubMed Google Scholar
Vergniol, J. et al. Noninvasive tests for fibrosis and liver stiffness predict 5-year outcomes of patients with chronic hepatitis C. Gastroenterology140, 1970–1979 (2011). ArticlePubMed Google Scholar
Robic, M. A. et al. Liver stiffness accurately predicts portal hypertension related complications in patients with chronic liver disease: A prospective study. J. Hepatol.55, 1017–1024 (2011). ArticlePubMed Google Scholar
Kim, D., Kim, W. R., Kim, H. J. & Therneau, T. M. Association between noninvasive fibrosis markers and mortality among adults with nonalcoholic fatty liver disease in the United States. Hepatology57, 1357–1365 (2013). ArticleCASPubMed Google Scholar
Treeprasertsuk, S., Bjornsson, E., Enders, F., Suwanwalaikorn, S. & Lindor, K. D. NAFLD fibrosis score: a prognostic predictor for mortality and liver complications among NAFLD patients. World J. Gastroenterol.19, 1219–1229 (2013). ArticlePubMedPubMed Central Google Scholar
Castera, L. & Pinzani, M. Biopsy and non-invasive methods for the diagnosis of liver fibrosis: does it take two to tango? Gut59, 861–866 (2010). ArticlePubMed Google Scholar