Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation (original) (raw)
- Brief Communication
- Published: 08 June 2008
- Matthew S Macauley2,
- Yuan He1,
- David L Shen2,
- David J Vocadlo2 &
- …
- Gideon J Davies1
Nature Structural & Molecular Biology volume 15, pages 764–765 (2008)Cite this article
- 1897 Accesses
- 89 Citations
- 7 Altmetric
- Metrics details
Abstract
_N_-Acetylglucosamine (_O_-GlcNAc) modification of proteins provides a mechanism for the control of diverse cellular processes through a dynamic interplay with phosphorylation. UDP-GlcNAc:polypeptidyl transferase (OGT) catalyzes _O_-GlcNAc addition. The structure of an intact OGT homolog and kinetic analysis of human OGT variants reveal a contiguous superhelical groove that directs substrates to the active site.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
Accession codes
Primary accessions
Protein Data Bank
References
- Hart, G.W., Housley, M.P. & Slawson, C. Nature 446, 1017–1022 (2007).
Article CAS Google Scholar - Wells, L., Vosseller, K. & Hart, G.W. Science 291, 2376–2378 (2001).
Article CAS Google Scholar - Vosseller, K., Wells, L., Lane, M.D. & Hart, G.W. Proc. Natl. Acad. Sci. USA 99, 5313–5318 (2002).
Article CAS Google Scholar - Kreppel, L.K., Blomberg, M.A. & Hart, G.W. J. Biol. Chem. 272, 9308–9315 (1997).
Article CAS Google Scholar - Lubas, W.A. & Hanover, J.A. J. Biol. Chem. 275, 10983–10988 (2000).
Article CAS Google Scholar - Lazarus, B.D., Roos, M.D. & Hanover, J.A. J. Biol. Chem. 280, 35537–35544 (2005).
Article CAS Google Scholar - Gross, B.J., Kraybill, B.C. & Walker, S. J. Am. Chem. Soc. 127, 14588–14589 (2005).
Article CAS Google Scholar - Lazarus, B.D., Love, D.C. & Hanover, J.A. Glycobiology 16, 415–421 (2006).
Article CAS Google Scholar - Jinek, M. et al. Nat. Struct. Mol. Biol. 11, 1001–1007 (2004).
Article CAS Google Scholar - Lairson, L., Henrissat, B., Davies, G.J. & Withers, S.G. Annu. Rev. Biochem. published online, doi:10.1146/annurev.biochem.76.061005.092322 (14 April 2008).
- Yang, X.Y. et al. Nature 451, 964–969 (2008).
Article CAS Google Scholar - Conti, E. & Kuriyan, J. Structure 8, 329–338 (2000).
Article CAS Google Scholar
Acknowledgements
This work was funded by the UK Biotechnology and Biological Sciences Research Council and the the Canadian Institutes of Health Research. G.J.D. is a Royal Society/Wolfson research Merit Award recipient. D.J.V. is a Canada Research Chair in chemical glycobiology and a fellow of the Michael Smith Foundation for Health Research (MSFHR). M.S.M. is a scholar of the MSFHR.
Author information
Authors and Affiliations
- Department of Chemistry, Structural Biology Laboratory, The University of York, Heslington, YO10 5YW, York, UK
Carlos Martinez-Fleites, Yuan He & Gideon J Davies - Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, V5A 1S6, British Columbia, Canada
Matthew S Macauley, David L Shen & David J Vocadlo
Authors
- Carlos Martinez-Fleites
You can also search for this author inPubMed Google Scholar - Matthew S Macauley
You can also search for this author inPubMed Google Scholar - Yuan He
You can also search for this author inPubMed Google Scholar - David L Shen
You can also search for this author inPubMed Google Scholar - David J Vocadlo
You can also search for this author inPubMed Google Scholar - Gideon J Davies
You can also search for this author inPubMed Google Scholar
Corresponding authors
Correspondence toDavid J Vocadlo or Gideon J Davies.
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–4, Supplementary Tables 1 and 2, and Supplementary Methods (PDF 5615 kb)
Supplementary Movie 1
Rotating view of the XcOGT (cyan)/Human TPR domain (gold) model with an importin-derived peptide in yellow and UDP in red. (AVI 8436 kb)
Rights and permissions
About this article
Cite this article
Martinez-Fleites, C., Macauley, M., He, Y. et al. Structure of an _O_-GlcNAc transferase homolog provides insight into intracellular glycosylation.Nat Struct Mol Biol 15, 764–765 (2008). https://doi.org/10.1038/nsmb.1443
- Received: 29 February 2008
- Accepted: 09 May 2008
- Published: 08 June 2008
- Issue Date: July 2008
- DOI: https://doi.org/10.1038/nsmb.1443