A second binding site for double-stranded RNA in TLR3 and consequences for interferon activation (original) (raw)
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- Published: 22 June 2008
Nature Structural & Molecular Biology volume 15, pages 761–763 (2008)Cite this article
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Abstract
We show that substrate specificity of Toll-like receptor 3 (TLR3) is due to the presence of two binding sites in the ectodomain, separated by 50 Å. This corresponds to two turns of a double-stranded RNA duplex, allowing differentiation between nucleic acids in the A- or B-type conformation. We propose that there are different arrangements of TLR3 ectodomains along the double-stranded RNA that could modulate the strength of the interferon response.
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Acknowledgements
This work was funded by the Slovenian Research Agency. We would like to thank J. Hiscott (McGill University) for his kind gift of interferon-β luciferase reporter plasmid.
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Authors and Affiliations
- Department of Biotechnology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, 1000, Slovenia
Nina Pirher, Karolina Ivičak, Jelka Pohar, Mojca Benčina & Roman Jerala - Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, 1000, Slovenia
Roman Jerala
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- Nina Pirher
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Correspondence toRoman Jerala.
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Pirher, N., Ivičak, K., Pohar, J. et al. A second binding site for double-stranded RNA in TLR3 and consequences for interferon activation.Nat Struct Mol Biol 15, 761–763 (2008). https://doi.org/10.1038/nsmb.1453
- Received: 08 April 2008
- Accepted: 29 May 2008
- Published: 22 June 2008
- Issue Date: July 2008
- DOI: https://doi.org/10.1038/nsmb.1453