IDN1 and IDN2 are required for de novo DNA methylation in Arabidopsis thaliana (original) (raw)

Nature Structural & Molecular Biology volume 16, pages 1325–1327 (2009)Cite this article

Abstract

DNA methylation is an epigenetic mark affecting genes and transposons. Screening for mutants that fail to establish DNA methylation yielded two we termed “_involved in de novo_” (idn) 1 and 2. IDN1 encodes DMS3, an SMC-related protein, and IDN2 encodes a previously unknown double-stranded RNA–binding protein with homology to SGS3. IDN1 and _IDN_2 control de novo methylation and small interfering RNA (siRNA)-mediated maintenance methylation and are components of the RNA-directed DNA methylation pathway.

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References

  1. Chan, S.W. et al. Science 303, 1336 (2004).
    Article CAS Google Scholar
  2. Cao, X. & Jacobsen, S.E. Curr. Biol. 12, 1138–1144 (2002).
    Article CAS Google Scholar
  3. Henderson, I.R. & Jacobsen, S.E. Nature 447, 418–424 (2007).
    Article CAS Google Scholar
  4. Li, C.F. et al. Cell 126, 93–106 (2006).
    Article CAS Google Scholar
  5. Pontes, O. et al. Cell 126, 79–92 (2006).
    Article CAS Google Scholar
  6. Wierzbicki, A.T., Haag, J.R. & Pikaard, C.S. Cell 135, 635–648 (2008).
    Article CAS Google Scholar
  7. Soppe, W.J. et al. Mol. Cell 6, 791–802 (2000).
    Article CAS Google Scholar
  8. Henderson, I.R. & Jacobsen, S.E. Genes Dev. 22, 1597–1606 (2008).
    Article CAS Google Scholar
  9. Cao, X. & Jacobsen, S.E. Proc. Natl. Acad. Sci. USA 99 Suppl 4, 16491–16498 (2002).
    Article CAS Google Scholar
  10. Kanno, T. et al. Nat. Genet. 40, 670–675 (2008).
    Article CAS Google Scholar
  11. Bateman, A. BMC Bioinformatics 3, 21 (2002).
    Article Google Scholar
  12. Mourrain, P. et al. Cell 101, 533–542 (2000).
    Article CAS Google Scholar
  13. Zhang, D. & Trudeau, V.L. Cell Cycle 7, 2268–2270 (2008).
    Article CAS Google Scholar
  14. Fukunaga, R. & Doudna, J.A. EMBO J. 28, 545–555 (2009).
    Article CAS Google Scholar

Download references

Acknowledgements

We thank J. Goodrich and D. Schubert for kindly providing T-DNA homozygous lines. We thank members of the Jacobsen laboratory for supportive discussions. Jacobsen lab research was supported by US National Institutes of Health grant GM60398. I.A. was supported by a postdoctoral fellowship from the Ministerio de Educacion y Ciencia. S.E.J. and J.C. are investigators of the Howard Hughes Medical Institute.

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Authors and Affiliations

  1. Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, California, USA
    Israel Ausin & Steven E Jacobsen
  2. Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, USA
    Todd C Mockler
  3. Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA
    Joanne Chory
  4. Howard Hughes Medical Institute, La Jolla, California, USA
    Joanne Chory
  5. Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California, USA
    Steven E Jacobsen

Authors

  1. Israel Ausin
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  2. Todd C Mockler
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  3. Joanne Chory
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  4. Steven E Jacobsen
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Contributions

T.C.M. and J.C. provided the majority of the insertion mutagenized population. I.A. and S.E.J. designed the experiments. I.A. performed the experiments and wrote the manuscript.

Corresponding author

Correspondence toSteven E Jacobsen.

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Ausin, I., Mockler, T., Chory, J. et al. IDN1 and IDN2 are required for de novo DNA methylation in Arabidopsis thaliana.Nat Struct Mol Biol 16, 1325–1327 (2009). https://doi.org/10.1038/nsmb.1690

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