Oriented loading of FtsK on KOPS (original) (raw)
- Brief Communication
- Published: 15 October 2006
- Omar A Saleh3,4,5,
- François Cornet1,
- Jean-François Allemand3,4 &
- …
- François–Xavier Barre2
Nature Structural & Molecular Biology volume 13, pages 1026–1028 (2006)Cite this article
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Abstract
In Escherichia coli, the ATP-dependent DNA translocase FtsK transports DNA across the site of cell division and activates recombination by the XerCD recombinases at a specific site on the chromosome, dif, to ensure the last stages of chromosome segregation. DNA transport by FtsK is oriented by 8-base-pair asymmetric sequences ('KOPS'). Here we provide evidence that KOPS promote FtsK loading on DNA and that translocation is oriented at this step.
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Acknowledgements
S.B. and O.A.S. were supported by a MENESR grant and an EU MolSwitch grant, respectively. Research was supported by the Centre national pour la recherche scientifique (ATIP), the French Ministry of Research (DRAB), the Fondation pour la Recherche Médicale and the Agence Nationale de la Recherche. We thank B. Michel, B. Lestrade, O. Espeli and C. Lesterlin for helpful comments.
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Authors and Affiliations
- Laboratoire de Microbiologie et de Génétique Moléculaire, CNRS, UMR5100, Toulouse, France
Sarah Bigot & François Cornet - Centre de Génétique Moléculaire, CNRS, UPR2167, Gif-sur-Yvette, France
Sarah Bigot & François–Xavier Barre - Laboratoire de Physique Statistique et Département de Biologie, Ecole Normale Supérieure, UMR8550 associé au CNRS et aux Universités Paris VI et VII, Paris, France
Omar A Saleh & Jean-François Allemand - Département de Chimie, Laboratoire Pasteur, Ecole Normale Supérieure, Paris, France
Omar A Saleh & Jean-François Allemand - Materials Department and Biomolecular Science and Engineering Program, University of California, Santa Barbara, California, USA
Omar A Saleh
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- Sarah Bigot
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Contributions
DNA engineering, protein purification and bulk analysis were performed by S.B., F.C. and F.-X.B., and single-molecule studies by O.A.S. and J.-F.A.
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Correspondence toFrançois–Xavier Barre.
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The authors declare no competing financial interests.
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Bigot, S., Saleh, O., Cornet, F. et al. Oriented loading of FtsK on KOPS.Nat Struct Mol Biol 13, 1026–1028 (2006). https://doi.org/10.1038/nsmb1159
- Received: 30 June 2006
- Accepted: 27 September 2006
- Published: 15 October 2006
- Issue Date: 01 November 2006
- DOI: https://doi.org/10.1038/nsmb1159
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