Structural basis for the coevolution of a viral RNA–protein complex (original) (raw)

Nature Structural & Molecular Biology volume 15, pages 103–105 (2008)Cite this article

Abstract

The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein–RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to selectively bind its cognate RNA, and it demonstrates that the conserved β-sheet surface is a flexible architecture that can evolve to recognize diverse RNA hairpins.

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References

  1. Kozak, M. & Nathans, D. Bacteriol. Rev. 36, 109–134 (1972).
    CAS PubMed PubMed Central Google Scholar
  2. Olsthoorn, R.C., Garde, G., Dayhuff, T., Atkins, J.F. & Van Duin, J. Virology 206, 611–625 (1995).
    Article CAS PubMed Google Scholar
  3. Carey, J., Cameron, V., de Haseth, P.L. & Uhlenbeck, O.C. Biochemistry 22, 2601–2610 (1983).
    Article CAS PubMed Google Scholar
  4. Lim, F., Downey, T.P. & Peabody, D.S. J. Biol. Chem. 276, 22507–22513 (2001).
    Article CAS PubMed Google Scholar
  5. Spingola, M. & Peabody, D.S. Nucleic Acids Res. 25, 2808–2815 (1997).
    Article CAS PubMed PubMed Central Google Scholar
  6. Lim, F., Spingola, M. & Peabody, D.S. J. Biol. Chem. 271, 31839–31845 (1996).
    Article CAS PubMed Google Scholar
  7. Horn, W.T. et al. Structure 14, 487–495 (2006).
    Article CAS PubMed PubMed Central Google Scholar
  8. Valegard, K., Liljas, L., Fridborg, K. & Unge, T. Nature 345, 36–41 (1990).
    Article CAS PubMed Google Scholar
  9. Ni, C.Z. et al. Structure 3, 255–263 (1995).
    Article CAS PubMed Google Scholar
  10. Golmohammadi, R., Fridborg, K., Bundule, M., Valegard, K. & Liljas, L. Structure 4, 543–554 (1996).
    Article CAS PubMed Google Scholar
  11. Tars, K., Bundule, M., Fridborg, K. & Liljas, L. J. Mol. Biol. 271, 759–773 (1997).
    Article CAS PubMed Google Scholar
  12. Lim, F. & Peabody, D.S. Nucleic Acids Res. 30, 4138–4144 (2002).
    Article CAS PubMed PubMed Central Google Scholar
  13. Valegard, K. et al. J. Mol. Biol. 270, 724–738 (1997).
    Article CAS PubMed Google Scholar
  14. Wu, H.N. & Uhlenbeck, O.C. Biochemistry 26, 8221–8227 (1987).
    Article CAS PubMed Google Scholar
  15. Valegard, K., Murray, J.B., Stockley, P.G., Stonehouse, N.J. & Liljas, L. Nature 371, 623–626 (1994).
    Article CAS PubMed Google Scholar
  16. Bardwell, V.J. & Wickens, M. Nucleic Acids Res. 18, 6587–6594 (1990).
    Article CAS PubMed PubMed Central Google Scholar
  17. Hogg, J.R. & Collins, K. RNA 13, 868–880 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  18. Lykke-Andersen, J., Shu, M.D. & Steitz, J.A. Cell 103, 1121–1131 (2000).
    Article CAS PubMed Google Scholar
  19. Bertrand, E. et al. Mol. Cell 2, 437–445 (1998).
    Article CAS PubMed Google Scholar

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Acknowledgements

This work was supported by the US National Institutes of Health (grants AR-41480 and EB-002060 to R.H.S and National Research Service Award institutional training grant (5T32HL007675-19) support to J.A.C.) and the Albert Einstein Cancer Center. The authors wish to thank the staff at the National Synchrotron Light Source X29a beamline for assistance with data collection, the staff at the Argonne Advanced Photon Source Structural GenomiX Collaborative Access Team beamline for express crystallography data collection and G. Arenas, M. Hennig, U. Meier, S. Nguyen and S. Ryder for helpful discussions.

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Authors and Affiliations

  1. Departments of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, 10461, New York, USA
    Jeffrey A Chao & Robert H Singer
  2. Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, 10461, New York, USA
    Yury Patskovsky & Steven C Almo

Authors

  1. Jeffrey A Chao
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  2. Yury Patskovsky
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  3. Steven C Almo
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  4. Robert H Singer
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Contributions

J.A.C. designed and performed the experiments. Y.P. assisted with crystallography. J.A.C., Y.P., S.C.A. and R.H.S. wrote and discussed the manuscript.

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Correspondence toRobert H Singer.

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Chao, J., Patskovsky, Y., Almo, S. et al. Structural basis for the coevolution of a viral RNA–protein complex.Nat Struct Mol Biol 15, 103–105 (2008). https://doi.org/10.1038/nsmb1327

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