ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane (original) (raw)
- Brief Communication
- Published: 23 October 2005
- Srinivas Mullapudi2,
- Thomas Becker1,
- Johanna Dudek3,
- Richard Zimmermann3,
- Pawel A Penczek2 &
- …
- Roland Beckmann1
Nature Structural & Molecular Biology volume 12, pages 1015–1016 (2005)Cite this article
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Abstract
Ribosomes translating secretory and membrane proteins are targeted to the endoplasmic reticulum membrane and attach to the protein-conducting channel and ribosome-associated membrane proteins (RAMPs). Recently, a new RAMP, ERj1p, has been identified that recruits BiP to ribosomes and regulates translational activity. Here we present the cryo-EM structure of a ribosome–ERj1p complex, revealing how ERj1p coordinates the ribosome at the membrane and how allosteric effects may mediate ERj1p's regulatory activity.
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Acknowledgements
This work was supported by grants from the VolkswagenStiftung (R.B.), Deutsche Forschungsgemeinschaft (R.Z., J.D. and grant Sfb449 to M.B. and R.B.) and from the US National Institutes of Health (R01 NS 43258 and R01 GM 60635 to P.A.P.). The technical assistance of A. Müller is gratefully acknowledged. The cryo-EM data were collected, in part, on the JEOL 3000SFF electron microscope at the National Center for Macromolecular Imaging (Houston, USA), which is supported by the US National Institutes of Health though the National Center for Research Resources's P41 program (grant RR02250). We thank A. Paredes for his microscopy work.
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Authors and Affiliations
- Institut für Biochemie der Charité, University Medical School Berlin, Monbijoustr. 2, Berlin, 10117, Germany
Michael Blau, Thomas Becker & Roland Beckmann - The University of Texas at Houston Medical School, 6431 Fannin, Houston, 77030, Texas, USA
Srinivas Mullapudi & Pawel A Penczek - Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Homburg, D-66421, Germany
Johanna Dudek & Richard Zimmermann
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- Michael Blau
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Correspondence toRoland Beckmann.
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Blau, M., Mullapudi, S., Becker, T. et al. ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane.Nat Struct Mol Biol 12, 1015–1016 (2005). https://doi.org/10.1038/nsmb998
- Received: 10 June 2005
- Accepted: 08 September 2005
- Published: 23 October 2005
- Issue Date: 01 November 2005
- DOI: https://doi.org/10.1038/nsmb998