ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane (original) (raw)

Nature Structural & Molecular Biology volume 12, pages 1015–1016 (2005)Cite this article

Abstract

Ribosomes translating secretory and membrane proteins are targeted to the endoplasmic reticulum membrane and attach to the protein-conducting channel and ribosome-associated membrane proteins (RAMPs). Recently, a new RAMP, ERj1p, has been identified that recruits BiP to ribosomes and regulates translational activity. Here we present the cryo-EM structure of a ribosome–ERj1p complex, revealing how ERj1p coordinates the ribosome at the membrane and how allosteric effects may mediate ERj1p's regulatory activity.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 12 print issues and online access

$209.00 per year

only $17.42 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

Accession codes

Primary accessions

Electron Microscopy Data Bank

References

  1. Brightman, S.E., Blatch, G.L. & Zetter, B.R. Gene 153, 249–254 (1995).
    CAS PubMed Google Scholar
  2. Kroczynska, B., Evangelista, C.M., Samant, S.S., Elguindi, E.C. & Blond, S.Y. J. Biol. Chem. 279, 11432–11443 (2004).
    CAS PubMed Google Scholar
  3. Dudek, J. et al. EMBO J. 21, 2958–2967 (2002).
    CAS PubMed PubMed Central Google Scholar
  4. Chevalier, M., Rhee, H., Elguindi, E.C. & Blond, S.Y. J. Biol. Chem. 275, 19620–19627 (2000).
    CAS PubMed PubMed Central Google Scholar
  5. Halic, M. et al. Nature 427, 808–814 (2004).
    CAS PubMed Google Scholar
  6. Dudek, J. et al. Nature Struct. Mol. Biol., advance online publication 23 October 2005 (10.1038/nsmb1002).
  7. Zupicich, J., Brenner, S.E. & Skarnes, W.C. Genome Biol. 2, Research 0050 (2001).
  8. Spahn, C.M. et al. Cell 107, 373–386 (2001).
    CAS PubMed Google Scholar
  9. Beckmann, R. et al. Cell 107, 361–372 (2001).
    CAS PubMed Google Scholar
  10. Ferbitz, L. et al. Nature 431, 590–596 (2004).
    CAS PubMed Google Scholar
  11. Nakatogawa, H. & Ito, K. Cell 108, 629–636 (2002).
    CAS PubMed Google Scholar

Download references

Acknowledgements

This work was supported by grants from the VolkswagenStiftung (R.B.), Deutsche Forschungsgemeinschaft (R.Z., J.D. and grant Sfb449 to M.B. and R.B.) and from the US National Institutes of Health (R01 NS 43258 and R01 GM 60635 to P.A.P.). The technical assistance of A. Müller is gratefully acknowledged. The cryo-EM data were collected, in part, on the JEOL 3000SFF electron microscope at the National Center for Macromolecular Imaging (Houston, USA), which is supported by the US National Institutes of Health though the National Center for Research Resources's P41 program (grant RR02250). We thank A. Paredes for his microscopy work.

Author information

Authors and Affiliations

  1. Institut für Biochemie der Charité, University Medical School Berlin, Monbijoustr. 2, Berlin, 10117, Germany
    Michael Blau, Thomas Becker & Roland Beckmann
  2. The University of Texas at Houston Medical School, 6431 Fannin, Houston, 77030, Texas, USA
    Srinivas Mullapudi & Pawel A Penczek
  3. Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Homburg, D-66421, Germany
    Johanna Dudek & Richard Zimmermann

Authors

  1. Michael Blau
    You can also search for this author inPubMed Google Scholar
  2. Srinivas Mullapudi
    You can also search for this author inPubMed Google Scholar
  3. Thomas Becker
    You can also search for this author inPubMed Google Scholar
  4. Johanna Dudek
    You can also search for this author inPubMed Google Scholar
  5. Richard Zimmermann
    You can also search for this author inPubMed Google Scholar
  6. Pawel A Penczek
    You can also search for this author inPubMed Google Scholar
  7. Roland Beckmann
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toRoland Beckmann.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Rights and permissions

About this article

Cite this article

Blau, M., Mullapudi, S., Becker, T. et al. ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane.Nat Struct Mol Biol 12, 1015–1016 (2005). https://doi.org/10.1038/nsmb998

Download citation

This article is cited by