Oblique-plane single-molecule localization microscopy for tissues and small intact animals (original) (raw)

Data availability

The data that support the findings of this study are available from the corresponding authors upon reasonable request.

Code availability

The custom MATLAB codes for the localization analysis used in this study are available as Supplementary Software.

Change history

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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Acknowledgements

The authors thank L. Li (X. Zhang Lab at the University of California, Berkeley) for providing gold-evaporated silicon wafer mirrors, S. Köhler (A.F. Dernburg Lab at the University of California, Berkeley) for help with C. elegans samples and A. Bormann and T. Square (C.T. Miller Lab at the University of California, Berkeley) for help with stickleback samples. We thank C.T. Miller, J.W. de Jong and H. Adesnik for discussions. X.Z. acknowledges support from the Gordon and Betty Moore Foundation and the Office of Naval Research Multidisciplinary University Research Initiative program (N00014-17-1-2588). K.X. is a Chan Zuckerberg Biohub investigator and acknowledges support from the Bakar Fellows Award, and STROBE, an NSF Science and Technology Center (DMR 1548924). M.W. acknowledges an NSF Graduate Research Fellowship (DGE-1106400).

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Authors and Affiliations

  1. NSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA
    Jeongmin Kim, Yuan Wang & Xiang Zhang
  2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
    Jeongmin Kim, Yuan Wang & Xiang Zhang
  3. Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA
    Michal Wojcik, Seonah Moon & Ke Xu
  4. Vision Science, School of Optometry, University of California, Berkeley, Berkeley, CA, USA
    Emilia A. Zin & John G. Flannery
  5. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA
    Nadia Marnani, Zachary L. Newman & John G. Flannery
  6. Division of Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
    Ke Xu
  7. Chan Zuckerberg Biohub, San Francisco, CA, USA
    Ke Xu

Authors

  1. Jeongmin Kim
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  2. Michal Wojcik
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  3. Yuan Wang
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  4. Seonah Moon
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  5. Emilia A. Zin
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  6. Nadia Marnani
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  7. Zachary L. Newman
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  8. John G. Flannery
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  9. Ke Xu
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  10. Xiang Zhang
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Contributions

J.K. designed and built the microscopy system, calculated theoretical PSFs, prepared fluorescent bead samples, calibrated the optical system and wrote software code for localization analysis. M.W. and S.M. prepared cell samples. S.M. labeled mouse brain tissues. E.A.Z. and J.G.F. prepared retina samples and provided fixed brain sections. N.M. and Z.L.N. prepared Drosophila samples. J.K. and M.W. carried out imaging experiments. J.K. and Y.W. analyzed single-molecule data. X.Z. and K.X. guided the research. J.K., M.W., Y.W., K.X. and X.Z. contributed to writing the manuscript.

Corresponding authors

Correspondence toKe Xu or Xiang Zhang.

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Competing interests

J.K., Y.W. and X.Z. have filed a provisional patent application on the microscopy system and method.

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Peer review information: Rita Strack was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.

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Kim, J., Wojcik, M., Wang, Y. et al. Oblique-plane single-molecule localization microscopy for tissues and small intact animals.Nat Methods 16, 853–857 (2019). https://doi.org/10.1038/s41592-019-0510-z

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