Amplification of the ABL gene in T-cell acute lymphoblastic leukemia (original) (raw)

Leukemia volume 18, pages 1153–1156 (2004)Cite this article

TO THE EDITOR

The Leukaemia Research Fund UK Cancer Cytogenetics Group Karyotype Database in Acute Leukaemia (Database)1 would like to inform readers of a new finding. We observed amplification involving the ABL gene in acute lymphoblastic leukemia (ALL), which appeared as multiple signals by fluorescence in situ hybridization (FISH) in eight patients with T-lineage ALL. Gene amplification is a frequent finding in a wide range of tumors but has been rarely described in acute leukemia. In cytogenetic preparations, it is usually seen intrachromosomally as homogeneously staining regions (HSR) or extrachromosomally as double minutes (dmin). FISH studies have revealed the genes involved in the amplified regions. Amplifications of the MYC and MLL genes have been previously reported in acute myeloid leukemia (AML),2,3,4 and of AML1 and MLL in ALL.3,5 Although amplification of the BCR/ABL fusion gene has been described in cases of chronic myeloid leukemia (CML) treated with imatinib mesylate, seen both as HSRs6 and dmins,7 amplification of ABL alone is rare. A six-fold and a 15-fold amplification of the gene have been described in the CML-derived cell line, K562,8 and in a patient with CML in lymphoid blast crisis, respectively.9 The only other FISH report of amplification involving ABL was in three cases of secondary AML.10

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Acknowledgements

We thank the Leukaemia Research Fund for financial support and the following UKCCG laboratories for providing samples for this study: Glasgow, Leeds, Leicester, Manchester, Nottingham, Cambridge, Salisbury and Oxford. We are also grateful to Dr M Rocchi (Resources in Molecular Cytogenetics, Bari, Italy) for kindly donating the _ABL_-specific PAC probes and to Dr John Crolla (Wessex Regional Genetics Laboratories, Salisbury) for growing and preparing these PAC probes as part of an ongoing collaborative study. This study could not have been performed without the dedication of the members of Medical Research Council Adult and Childhood Leukaemia Working Parties, who have designed and coordinated the clinical trials through which these patients were identified and on which they were treated.

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Authors and Affiliations

  1. Cancer Sciences Division, Leukaemia Research Fund Cytogenetics Group, University of Southampton, Southampton, UK
    K E Barber, M Martineau, L Harewood, Z J Broadfield, K L Cheung, R L Harris, G R Jalali, A V Moorman, H M Robinson & C J Harrison
  2. Institute of Comparative Medicine, Glasgow University Veterinary School, Glasgow, UK
    M Stewart & E Cameron
  3. The Orchid Cancer Appeal, Cancer Research UK Medical Oncology Unit, London, UK
    J C Strefford
  4. Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK
    S Rutherford & T D Allen

Authors

  1. K E Barber
  2. M Martineau
  3. L Harewood
  4. M Stewart
  5. E Cameron
  6. J C Strefford
  7. S Rutherford
  8. T D Allen
  9. Z J Broadfield
  10. K L Cheung
  11. R L Harris
  12. G R Jalali
  13. A V Moorman
  14. H M Robinson
  15. C J Harrison

Corresponding author

Correspondence toC J Harrison.

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Barber, K., Martineau, M., Harewood, L. et al. Amplification of the ABL gene in T-cell acute lymphoblastic leukemia.Leukemia 18, 1153–1156 (2004). https://doi.org/10.1038/sj.leu.2403357

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