A microRNA cluster as a target of genomic amplification in malignant lymphoma (original) (raw)

Leukemia volume 19, pages 2013–2016 (2005)Cite this article

TO THE EDITOR

MicroRNAs (miRNAs) represent small RNAs (20–24 nt) and non-coding RNAs that perform a multitude of functions.1 MiRNAs display dynamic temporal and spatial expression patterns, where disruption of these patterns might be associated with tumorigenesis. We recently found that C13orf25 is a target gene of 13q31–q32 gain/amplification in malignant lymphomas.2 C13orf25 encodes two variant transcripts by alternative splicing, referred to as C13orf25 transcript variants 1 and 2. Of particular interest are the seven miRNA genes (miR-17, miR-18, miR-19a, miR-19b, miR-20 and miR-92) clustered within the region of C13orf25 transcript variant 2 (C13orf25 v2). The expression of these miRNAs was investigated in the present study in an effort to determine whether these are also overexpressed concomitantly with C13orf25 v2 overexpression using 12 malignant lymphoma cell lines and 21 diffuse large B-cell lymphoma (DLBCL) cases. We recently reported array CGH analysis of genomic gains and losses of 66 cases of DLBCL.3 In all, 21 of the 66 cases were examined for real-time quantitative polymerase chain reaction (PCR) analysis in this study. Of the 21 cases, eight cases showed a genomic gain at 13q, two cases of which showed high copy number gain (amplification) at 13q31. The remaining 13 of the 21 cases showed no copy number changes at 13q.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Subscribe to this journal

Receive 12 print issues and online access

$259.00 per year

only $21.58 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

References

  1. Ambros V . The functions of animal microRNAs. Nature 2004; 431: 350–355.
    CAS PubMed Google Scholar
  2. Ota A, Tagawa H, Karnan S, Tsuzuki S, Karpas A, Kira S et al. Identification and characterization of a novel gene, C13orf25, as a target for 13q31–q32 amplification in malignant lymphoma. Cancer Res 2004; 64: 3087–3095.
    Article CAS PubMed Google Scholar
  3. Tagawa H, Tsuzuki S, Suzuk R, Karnan S, Ota A, Kameoka Y et al. Genome-wide array comparative genomic hybridization of diffuse large B-cell lymphoma: comparison between CD5-positive and CD5-negative cases. Cancer Res 2004; 64: 5948–5955.
    Article CAS PubMed Google Scholar
  4. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T . Identification of novel genes coding for small expressed RNAs. Science 2001; 294: 853–858.
    Article CAS PubMed Google Scholar
  5. Metzler M, Wilda M, Busch K, Viehmann S, Borkhardt A . High expression of precursor microRNA-155/BIC RNA in children with Burkitt lymphoma. Genes Chromosomes Cancer 2004; 39: 167–169.
    Article CAS PubMed Google Scholar
  6. Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E et al. Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci USA 2003; 99: 15524–15529.
    Article Google Scholar

Download references

Author information

Authors and Affiliations

  1. Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan
    H Tagawa & M Seto

Authors

  1. H Tagawa
    You can also search for this author inPubMed Google Scholar
  2. M Seto
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toH Tagawa.

Rights and permissions

About this article

Cite this article

Tagawa, H., Seto, M. A microRNA cluster as a target of genomic amplification in malignant lymphoma.Leukemia 19, 2013–2016 (2005). https://doi.org/10.1038/sj.leu.2403942

Download citation

This article is cited by