Identification of a new monoclonal B-cell subset in unaffected first-degree relatives in familial chronic lymphocytic leukemia (original) (raw)

Leukemia volume 19, pages 2339–2341 (2005)Cite this article

TO THE EDITOR

Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults in the Western world. In spite of recent immunophenotypic, cytogenetic and molecular advances in the characterization of CLL, the oncogenic and cellular pathways are still poorly understood. A monoclonal B-cell lymphocytosis (MBL) could represent, in a proportion of cases, an early stage of CLL,1, 2, 3 and an increased prevalence of MBL in first-degree relatives (FDR) of familial CLL compared to the general population has been reported.4, 5 In order to study MBL as the early precursor of CLL, we investigated peripheral blood from the unaffected FDR of familial CLL, using high-sensitivity, multicolor flow cytometry (MCFCM) techniques, that is, seven-color, nine parameters.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 12 print issues and online access

$259.00 per year

only $21.58 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

References

  1. Marti GE, Muller J, Stetler-Stevenson M, Caporaso N . Chapter 3, B-cell monoclonal lymphocytosis in three individuals living near a hazardous waste site. In: Marti GE, Vogt RF, Zenger VE (eds). Proceedings of the USPHS Workshop on Laboratory Approaches to Determining the Role of Environmental Exposures as Risk Factors for B-Cell Chronic Lymphocytic Leukemia and Other B-Cell Lymphoproliferative Disorders. Atlanta, GA: US Department of Health and Human Services, 1997, pp 37–50.
    Google Scholar
  2. Rawstron AC, Green MJ, Kuzmicki A, Kennedy B, Fenton JA, Evans PA et al. Monoclonal B lymphocytes with the characteristics of ‘indolent’ chronic lymphocytic leukemia are present in 3.5% of adults with normal blood counts. Blood 2002; 100: 635–639.
    Article CAS Google Scholar
  3. Ghia P, Prato G, Scielzo C, Stella S, Geuna M, Guida G et al. Monoclonal CD5+ and CD5− B lymphocyte expansions are frequent in the peripheral blood of the elderly. Blood 2004; 103: 2337–2342.
    Article CAS Google Scholar
  4. Marti GE, Carter P, Abbasi F, Washington GC, Jain N, Zenger VE et al. B-cell monoclonal lymphocytosis and B-cell abnormalities in the setting of familial B-cell chronic lymphocytic leukemia. Cytometry (Clin Cytometry) 2003; 52B: 1–12.
    Article Google Scholar
  5. Rawstron AC, Yuille MR, Fuller J, Cullen M, Kennedy B, Richards SJ et al. Inherited predisposition to CLL is detectable as sub-clinical monoclonal B-lymphocyte expansion. Blood 2002; 100: 2289–2291.
    Article CAS Google Scholar
  6. Liu YJ, de Bouteiller O, Arpin C, Briere F, Galibert L, Ho S et al. Normal human IgD+IgM− germinal center B cells can express up to 80 mutations in the variable region of their IgD transcripts. Immunity 1996; 4: 603–613.
    Article CAS Google Scholar
  7. Arpin C, de Bouteiller O, Razanajaona D, Briere F, Banchereau J, Lebecque S et al. Human peripheral B cell development. sIgM−IgD+CD38+ hypermutated germinal center centroblasts preferentially express Ig lambda light chain and have undergone mu-to-delta switch. Ann N Y Acad Sci 1997; 815: 193–198.
    Article CAS Google Scholar
  8. Arpin C, de Bouteiller O, Razanajaona D, Fugier-Vivier I, Briere F, Banchereau J et al. The normal counterpart of IgD myeloma cells in germinal center displays extensively mutated IgVH gene, Cmu-Cdelta switch, and lambda light chain expression. J Exp Med 1998; 187: 1169–1178.
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Institut Paoli-Calmettes, Marseille, France
    T Aurran-Schleinitz
  2. Flow Cytometry Core Facility, ETIB, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    W Telford
  3. Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA
    S Perfetto
  4. Genetics Epidemiology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    N Caporaso
  5. MOCRU, CCR, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    W Wilson
  6. Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    M A Stetler-Stevenson
  7. Division of Cell and Gene Therapies, Office of Cellular, Tissues and Gene Therapy, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA
    T Aurran-Schleinitz, V E Zenger, F Abbasi & G E Marti

Authors

  1. T Aurran-Schleinitz
    You can also search for this author inPubMed Google Scholar
  2. W Telford
    You can also search for this author inPubMed Google Scholar
  3. S Perfetto
    You can also search for this author inPubMed Google Scholar
  4. N Caporaso
    You can also search for this author inPubMed Google Scholar
  5. W Wilson
    You can also search for this author inPubMed Google Scholar
  6. M A Stetler-Stevenson
    You can also search for this author inPubMed Google Scholar
  7. V E Zenger
    You can also search for this author inPubMed Google Scholar
  8. F Abbasi
    You can also search for this author inPubMed Google Scholar
  9. G E Marti
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toG E Marti.

Rights and permissions

About this article

Cite this article

Aurran-Schleinitz, T., Telford, W., Perfetto, S. et al. Identification of a new monoclonal B-cell subset in unaffected first-degree relatives in familial chronic lymphocytic leukemia.Leukemia 19, 2339–2341 (2005). https://doi.org/10.1038/sj.leu.2403980

Download citation