Inhibitory effects of omega-3 fatty acids on protein kinase C activity in vitro (original) (raw)
- Original Research Article
- Published: 17 April 2001
Molecular Psychiatry volume 6, pages 246–248 (2001)Cite this article
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Abstract
Preliminary clinical data indicate that omega-3 fatty acids may be effective mood stabilizers for patients with bipolar disorder. Both lithium and valproic acid are known to inhibit protein kinase C (PKC) activity after subchronic administration in cell culture and in vivo. The current study was undertaken to determine the effects of the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on protein kinase C phosphotransferase activity in vitro. Various concentrations of DHA, EPA, and arachidonic acid (AA) were incubated with the catalytic domain of protein kinase C beta from rat brain. Protein kinase C activity was measured by quantifying incorporation of 32P-PO4 into a synthetic peptide substrate. Both DHA and EPA, as well as the combination of DHA and EPA, inhibited PKC activity at concentrations as low as 10 μmol l−1. In contrast, arachidonic acid had no effect on PKC activity. Thus, PKC represents a potential site of action of omega-3 fatty acids in their effects on the treatment of bipolar disorder.
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Acknowledgements
Financial Support: NARSAD Independent Investigator Award (DJS), NIH R01AT00161–01 (LBM, ALS). These data were presented in part at the Society of Biological Psychiatry Annual Meeting, May 2000.
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Authors and Affiliations
- Department of Psychiatry & Behavioral Sciences, Baylor College of Medicine, Houston, 77030, Texas, USA
H F Seung Kim & L B Marangell - Department of Neuroscience, Baylor College of Medicine, Houston, 77030, Texas, USA
E J Weeber & J D Sweatt - Psychopharmacology Research Laboratory, McLean Hospital, Belmont, MA, USA
A L Stoll - Department of Psychiatry, Harvard Medical School, Boston, MA, USA
A L Stoll
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- H F Seung Kim
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Correspondence toL B Marangell.
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Seung Kim, H., Weeber, E., Sweatt, J. et al. Inhibitory effects of omega-3 fatty acids on protein kinase C activity in vitro.Mol Psychiatry 6, 246–248 (2001). https://doi.org/10.1038/sj.mp.4000837
- Received: 12 July 2000
- Revised: 28 September 2000
- Accepted: 28 September 2000
- Published: 17 April 2001
- Issue Date: 01 March 2001
- DOI: https://doi.org/10.1038/sj.mp.4000837