Suppression of Interleukin-1β converting enzyme (ICE)-induced apoptosis by SV40 large T antigen (original) (raw)

Oncogene volume 14, pages 1207–1214 (1997)Cite this article

Abstract

The Interleukin-1β converting enzyme (ICE) family of proteins, homologs of the C elegans cell death gene product CED-3, play important roles in controlling vertebrate programmed cell death. Because inhibition of apoptosis may be an essential step in tumorigenesis, we investigated the interaction of the simian virus 40 large T antigen (T ag) with the ICE family. COS-1 cells which were transformed by the simian virus 40 do not die when transfected with expression constructs of Ice or Ich-1 L. We found that expression of T ag alone significantly prevents the ICE-induced apoptosis. p53, but not pRb or p107, antagonizes the effect of T ag on the suppression of ICE-induced cell death, but not on ICH-1L-mediated cell death. Thus, wild type p53 may potentiate ICE-induced apoptosis. Expression of a temperature sensitive mutant p53Val135 sensitizes COS-1 cells to apoptosis induced by ICE at permissive but not at non-permissive temperature. While induction of bax, p21 WAF1/CIP, or cyclin D1 gene expression is observed in the COS-1 p53Val135 cells at the permissive temperature, overexpression of bax, but not p21 WAF1/CIP or cyclin D1, potentiates ICE-induced COS-1 cell death. Taken together, these results suggest that T ag may modulate the cells' susceptibility to death by suppressing activity of the ICE family through inhibiting p53.

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  1. Yong-Keun Jung & Junying Yuan
    Present address: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA02115, USA

Authors and Affiliations

  1. Cardiovascular Research Center, Massachusetts General Hospital-East, Charlestown, 02129, Massachusetts
    Yong-Keun Jung
  2. Department of Medicine, Harvard Medical School, Boston, 02115, Massachusetts
    Junying Yuan

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  1. Yong-Keun Jung
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  2. Junying Yuan
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Jung, YK., Yuan, J. Suppression of Interleukin-1β converting enzyme (ICE)-induced apoptosis by SV40 large T antigen.Oncogene 14, 1207–1214 (1997). https://doi.org/10.1038/sj.onc.1200943

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