Cross-family interaction between the bHLHZip USF and bZip Fra1 proteins results in down-regulation of AP1 activity (original) (raw)

• Original Paper
• Published: 01 May 1997
• Kim E Boulukos1,
• Christel Aperlo1,
• Mitsuaki Fujimoto2,
• Hiroyoshi Ariga2,
• Akio Nomoto3 &
• …
• Hiroyuki Kato2 nAff4

Oncogene volume 14, pages 2091–2098 (1997)Cite this article

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Abstract

Heterodimerization among the basic-leucine zipper (bZIP) proteins or among the basic-helix – loop – helix-leucine zipper (bHLHZip) proteins confers a multitude of combinational activities to these transcription factors. To further examine the function of the bHLHZip protein, USF, we screened for cellular proteins which could directly interact with USF using the yeast two-hybrid system. A bZip protein, Fra1, was found to efficiently interact with USF. USF specifically interacts with Fra1 but not with other closely related family members, c-Fos, Fra2, FosB, or with c-Jun. Both the bHLHZip and the N-terminal regions of Fra1 are required for efficient interaction with USF. In vivo association between USF and Fra1 has been demonstrated by co-immunoprecipitation. Expression of exogenous USF led to a decrease in AP1-dependent transcription in F9 cells. Co-expression of exogenous Fra1 restored the AP1 activity in a dose-dependent manner. These data show that USF and Fra1 physically and functionally interact demonstrating that cross-talk occurs between factors of distantly related transcription families.

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Author notes

1. Hiroyuki Kato
   Present address: Department of Molecular Oncology, Nippon Roche Research Center, 200 Kajiwara, Kamakura, Kanagawa 247, Japan

Authors and Affiliations

1. Centre de Biochimie, Universite de Nice, Parc Valrose, Nice, cedex 2 06108, France
   Philippe Pognonec, Kim E Boulukos & Christel Aperlo
2. Department of Molecular Biology, Faculty of Pharmaceutical Sciences, Hokkaido University, N 12 W 6, Kita-ku, Sapporo, 060, Japan
   Mitsuaki Fujimoto, Hiroyoshi Ariga & Hiroyuki Kato
3. Department of Microbiology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan
   Akio Nomoto

Authors

1. Philippe Pognonec
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2. Kim E Boulukos
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3. Christel Aperlo
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4. Mitsuaki Fujimoto
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5. Hiroyoshi Ariga
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6. Akio Nomoto
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7. Hiroyuki Kato
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Pognonec, P., Boulukos, K., Aperlo, C. et al. Cross-family interaction between the bHLHZip USF and bZip Fra1 proteins results in down-regulation of AP1 activity.Oncogene 14, 2091–2098 (1997). https://doi.org/10.1038/sj.onc.1201046

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• Received: 03 June 1996
• Revised: 16 January 1997
• Accepted: 17 January 1997
• Issue Date: 01 May 1997
• DOI: https://doi.org/10.1038/sj.onc.1201046

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