Comprehensive allelotyping of human hepatocellular carcinoma (original) (raw)
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- Published: 19 June 1997
Oncogene volume 14, pages 2927–2933 (1997)Cite this article
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Abstract
Hepatocellular carcinoma (HCC) is one of the most common cancers in many parts of the world, however the molecular mechanisms underlying liver cell transformation remain obscure. A genome-wide scan of loss of heterozygosity (LOH) in tumors provides a powerful tool to search for genes involved in neoplastic processes. To identify recurrent genetic alterations in liver tumors, we examined DNAs isolated from 120 HCCs and their adjacent non tumorous parts for LOH using a collection of 195 microsatellite markers located roughly every 20 cM throughout 39 autosomal arms. The mean heterozygosity was 73%. Our findings provide additional support that LOH for loci on chromosomal arms 1p, 4q, 6q, 8p, 13q and 16p is significantly elevated in HCC. The highest percentage of LOH is found for a locus in 8p23 (42% of informative csaes). This corresponds to one of the most common genetic abnormalities reported to date in these tumors. In addition, high ratio of LOH (⩾35%) is observed on chromosome arms which had not been implicated in previous studies, notably on 1q, 2q and 9q. No correlation was found between LOH of specific chromosomal regions and etiologic factors such as chronic infections with hepatitis B or C viruses. This first report of an extensive allelotypic analysis of HCC should help in identifying new genes whose loss of function contributes to the development of liver cancer.
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Author notes
- Hisaki Nagai and Pascal Pineau: The first two authors should be considered as first co-authors
Authors and Affiliations
- Unité de Recombinaison et Expression Génétique, INSERM U163, Institut Pasteur, 28 rue du Dr Roux, Paris, Cedex 15 75724, France
Hisaki Nagai, Pascal Pineau, Pierre Tiollais, Marie Annick Buendia & Anne Dejean
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- Hisaki Nagai
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Nagai, H., Pineau, P., Tiollais, P. et al. Comprehensive allelotyping of human hepatocellular carcinoma.Oncogene 14, 2927–2933 (1997). https://doi.org/10.1038/sj.onc.1201136
- Received: 23 December 1996
- Revised: 03 March 1997
- Accepted: 03 March 1997
- Issue Date: 19 June 1997
- DOI: https://doi.org/10.1038/sj.onc.1201136