Absence of p53-dependent cell cycle regulation in pluripotent mouse cell lines (original) (raw)

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• Published: 16 June 1998

Oncogene volume 16, pages 3003–3011 (1998)Cite this article

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Abstract

We examined the expression of p53 in three lines of pluripotent embryonal carcinoma (EC) and ES cells. p53 mRNA and protein levels were constitutively high in two lines but absent from one. In the P19 line of EC cells neither p53 protein nor mRNA was detected. The first intron of the p53 gene in these cells had been invaded by a murine leukemia virus and there was extensive hypermethylation of the p53 gene accompanying its inactivation. In all three cell lines, irradiation resulted in arrest of the cells in the G2 but not in the G1 phase of the cell cycle despite the induction of p21cip1 in the cell lines expressing p53. Thus, the chromosomal stability of EC and ES cells appears to be not dependent on the p53 protein and we interpret our results to suggest that these cells may require the deletion of p53 dependent cell cycle regulation in order to become immortalized.

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Authors and Affiliations

1. Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa, 501 Smyth Road, Ottawa, K1H 8L6, Ontario, Canada
   Prabha K Schmidt-Kastner, Karen Jardine, Michelle Cormier & Michael W McBurney

Authors

1. Prabha K Schmidt-Kastner
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2. Karen Jardine
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3. Michelle Cormier
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4. Michael W McBurney
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Schmidt-Kastner, P., Jardine, K., Cormier, M. et al. Absence of p53-dependent cell cycle regulation in pluripotent mouse cell lines.Oncogene 16, 3003–3011 (1998). https://doi.org/10.1038/sj.onc.1201835

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• Received: 09 October 1997
• Revised: 07 January 1998
• Accepted: 08 January 1998
• Published: 16 June 1998
• Issue Date: 11 June 1998
• DOI: https://doi.org/10.1038/sj.onc.1201835

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