Interaction of radixin with Rho small G protein GDP/GTP exchange protein Dbl (original) (raw)

Oncogene volume 16, pages 3279–3284 (1998)Cite this article

Abstract

The Rho small G protein family, consisting of the Rho, Rac, and Cdc42 subfamilies, regulates various actin cytoskeleton-dependent cell functions. The Rho subfamily members regulate ERM (ezrin, radixin and moesin)-dependent association of the actin cytoskeleton with the plasma membrane. Moreover, the N-terminal regions of ERM interact with Rho GDI, an inhibitory regulator of all the Rho family members, and reduce its inhibitory action, finally initiating the activation of the Rho family members. We show here that the N-terminal region of radixin furthermore interacts with Dbl, a stimulatory GDP/GTP exchange protein of the Rho family members. This interaction does not affect the Dbl activity to stimulate the GDP/GTP exchange reaction of RhoA, a member of the Rho subfamily. Dbl does not interact with radixin which is precomplexed with Rho GDI, and Rho GDI displaces Dbl from radixin. Thus, radixin plays an important role in activation of the Rho family members by recruiting their positive and negative regulators.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 50 print issues and online access

$259.00 per year

only $5.18 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

Author information

Author notes

  1. Kazuo Takahashi
    Present address: The Second Department of Internal Medicine, Chiba University Medical School, Chiba, 260, Japan

Authors and Affiliations

  1. Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, 565, Japan
    Kazuo Takahashi, Takuya Sasaki, Akiko Mammoto, Ikuko Hotta, Kenji Takaishi, Hiroshi Imamura, Katsutoshi Nakano, Atsuko Kodama & Yoshimi Takai

Authors

  1. Kazuo Takahashi
    You can also search for this author inPubMed Google Scholar
  2. Takuya Sasaki
    You can also search for this author inPubMed Google Scholar
  3. Akiko Mammoto
    You can also search for this author inPubMed Google Scholar
  4. Ikuko Hotta
    You can also search for this author inPubMed Google Scholar
  5. Kenji Takaishi
    You can also search for this author inPubMed Google Scholar
  6. Hiroshi Imamura
    You can also search for this author inPubMed Google Scholar
  7. Katsutoshi Nakano
    You can also search for this author inPubMed Google Scholar
  8. Atsuko Kodama
    You can also search for this author inPubMed Google Scholar
  9. Yoshimi Takai
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Takahashi, K., Sasaki, T., Mammoto, A. et al. Interaction of radixin with Rho small G protein GDP/GTP exchange protein Dbl.Oncogene 16, 3279–3284 (1998). https://doi.org/10.1038/sj.onc.1201874

Download citation

Keywords

This article is cited by