Cloning and characterization of human and mouse telomerase RNA gene promoter sequences (original) (raw)
- Original Paper
- Published: 19 March 1998
- Stacey F Hoare1,
- Robert McFarlane2,
- Sharon Muir1,
- E Kenneth Parkinson2,
- Donald M Black2 &
- …
- W Nicol Keith1
Oncogene volume 16, pages 1345–1350 (1998)Cite this article
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Abstract
Variation in telomerase activity is correlated with cellular senescence and tumour progression. However, although the enzymatic activity of telomerase has been well studied, very little is known about how expression of telomerase genes is regulated in mammalian cells. We have therefore cloned the promoter regions of the human (hTR), and mouse, (terc), telomerase RNA genes in order to identify the regulatory elements controlling telomerase RNA gene transcription. 1.76 kb encompassing the hTR gene promoter region was sequenced, as was 4 kb encompassing the terc promoter. No significant sequence similarity could be detected in comparisons between human and mouse 5′-regions, flanking the transcribed sequences. However, both the human and mouse telomerase RNA genes are within CpG islands and may therefore be under the regulation of DNA methylation. Transient expression of hTR-reporter gene constructs in HeLa and GM847 cells identified the elements responsible for promoter activity are contained in a 231 bp region upstream of the transcriptional start site. Transient expression of **terc**-reporter gene constructs in Swiss3T3 and A9 cells identified the elements responsible for promoter activity are contained in a 73 bp region upstream of the transcriptional start site. These studies have implications for novel transcription targeted cancer therapies.
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Authors and Affiliations
- CRC Department of Medical Oncology, University of Glasgow, CRC Beatson Laboratories, Alexander Stone Building, Garscube Estate, Switchback Road, Bearsden, G61 1BD, Glasgow
Jiang-Qin Zhao, Stacey F Hoare, Sharon Muir & W Nicol Keith - Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, G61 1BD, Glasgow, UK
Robert McFarlane, E Kenneth Parkinson & Donald M Black
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- Jiang-Qin Zhao
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Zhao, JQ., Hoare, S., McFarlane, R. et al. Cloning and characterization of human and mouse telomerase RNA gene promoter sequences.Oncogene 16, 1345–1350 (1998). https://doi.org/10.1038/sj.onc.1201892
- Received: 17 December 1997
- Revised: 03 February 1998
- Accepted: 05 February 1998
- Published: 19 March 1998
- Issue Date: 12 March 1998
- DOI: https://doi.org/10.1038/sj.onc.1201892