p16INK4a inactivation is not required to immortalize human mammary epithelial cells (original) (raw)

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Published: 06 November 2002

Oncogene volume 21, pages 7897–7900 (2002) Cite this article

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Abstract

Using standard culture conditions, primary human mammary epithelial cells (HMECs) undergo a premature, transient growth arrest termed M0 (mortality stage 0) after 10–15 population doublings in vitro. It has been reported that emergence from this growth arrest by the abrogation of p16INK4a, a cyclin-dependent kinase inhibitor, and expression of the catalytic component of human telomerase (hTERT) are necessary for HMEC immortalization. Here we show that primary HMECs, grown on feeder layers, do not undergo this growth arrest and can be immortalized without abrogating p16. These findings support the concept that the so-called M0 stage represents a cell culture stress-induced growth arrest and that hTERT is sufficient to immortalize HMECs when cultured under adequate conditions.

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Acknowledgements

We thank Y Zou for cytogenetics assistance and C Passons and D LaRue for technical assistance. This work was supported by a fellowship from the USAMR DAMD Breast Cancer Research Program to B Herbert, a grant from the NCI, CADRG, DCP, under CN-05017-63, and the Ellison Medical Foundation. WE Wright and JW Shay are co-holders of the Southland Financial Corporation Distinguished Chair in Geriatric Research.

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Authors and Affiliations

Department of Cell Biology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, 75390-9039, Texas, TX, USA
Brittney-Shea Herbert, Woodring E Wright & Jerry W Shay

Authors

Brittney-Shea Herbert
Woodring E Wright
Jerry W Shay

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Correspondence toJerry W Shay.

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Herbert, BS., Wright, W. & Shay, J. p16INK4a inactivation is not required to immortalize human mammary epithelial cells.Oncogene 21, 7897–7900 (2002). https://doi.org/10.1038/sj.onc.1205902

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Received: 13 May 2002
Revised: 11 July 2002
Accepted: 18 July 2002
Published: 06 November 2002
Issue date: 07 November 2002
DOI: https://doi.org/10.1038/sj.onc.1205902