Peripheral blood mononuclear cell-based metabolomic profiling of a chronic unpredictable mild stress rat model of depression (original) (raw)
* Corresponding authors
a Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China
b Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China
c Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China
d Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Yongchuan Hospital of Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China
E-mail: xiepeng@cqmu.edu.cn
Fax: +86-23-68485111
Tel: +86-23-68485490
e Department of Clinical Laboratory Medicine, the Fifth People's Hospital of Chongqing, China
Abstract
Major depressive disorder (MDD) is a debilitating mood disorder with various etiopathological hypotheses. However the pathogenesis and diagnosis are still unclear. Peripheral blood mononuclear cells (PBMCs) have been shown to be well-suited to biomarker investigation in major depressive disorder (MDD), as well as to unveil the underlying pathogenesis of MDD. In this study, PBMCs were obtained from a chronic unpredictable mild stress (CUMS) rodent model of depression. A gas chromatography-mass spectrometry (GC/MS) metabolomic approach coupled with principal component analysis (PCA) and open partial least-squares discriminant analysis (OPLS-DA) statistical analysis was used to detect differential metabolites in PBMCs of depressed rats. A total number of 18 differential metabolites were screened out. Seven metabolites showed lower levels in CUMS relative to healthy control rats, including aspartic acid, glutamic acid, dehydroascorbic acid, aminomalonic acid, glycine, β-alanine, and ethanolamine, while eleven metabolites showed an increase in CUMS relative to healthy control rats, namely erythronic acid, fructose, β-tocopherol, adenosine-5-monophosphate, 5-hydroxytryptamine, 5-hydroxytryptamine, glycolic acid, α-tocopherol, tetradecanoic acid, creatinine, 4,5-dimethyl-2,6-dihydroxypyrimidine, and myo-inositol. These molecular changes were closely related to perturbations in neurotransmitter metabolism, energy metabolism and oxidative stress metabolism. Biochemical function analysis of these differential metabolites suggested that altered neurotransmitter, energy and oxidative metabolism disorder might be evolved in the pathogenesis of MDD, which could be of valuable assistance in the clinical diagnosis of MDD.
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Article information
DOI
https://doi.org/10.1039/C4MB00388H
Article type
Paper
Submitted
01 Jul 2014
Accepted
22 Aug 2014
First published
22 Aug 2014
Download Citation
Mol. BioSyst., 2014,10, 2994-3001
Author version available
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Peripheral blood mononuclear cell-based metabolomic profiling of a chronic unpredictable mild stress rat model of depression
J. Li, G. Tang, K. Cheng, D. Yang, G. Chen, Z. Liu, R. Zhang, J. Zhou, L. Fang, Z. Fang, X. Du and P. Xie,Mol. BioSyst., 2014, 10, 2994DOI: 10.1039/C4MB00388H
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